DNA Assembly Cloning and Mutagenesis Kits

Easy cloning of all PCR products, including blunt and TA ends

• In vitro transcription with both SP6 and T7 promoters
• BsaI-site removed from the ampicillin-resistant gene (allows for the cloning of Golden Gate Assembly modules)
• Fast cloning experiments with 5-minute ligation step
• Simplified screening with low/no colony background and no blue/white selection required

• Save time by eliminating purification steps
• Flanking restriction sites available for easy subcloning, including choice of two single digest options
• Provided analysis primers allow for downstream colony PCR screening or sequencing
• Ready-to-use kit components include 1 kb control amplicon, linearized cloning vector and single-use competent E. coli
• Longer shelf life (12 months), as compared to some commercially available products
• Also available with competent cells

The Q5^® Site-Directed Mutagenesis Kit (Without Competent Cells) enables rapid, site-specific mutagenesis of double-stranded plasmid DNA in less than 2 hours.

• Non-overlapping primer design ensures robust, exponential amplification, generating a high percentage of desired mutations from a wide range of templates
• Intramolecular ligation and transformation into NEB high-efficiency competent cells results in high colony yield
• Extremely low error rate of Q5 Hot Start High-Fidelity DNA Polymerase reduces screening time

• Hot start polymerase enables room temperature reaction setup
• DpnI background reduction permits a wide range of starting template concentrations
• Use of standard primers eliminates additional expenses from phosphorylated or purified oligos
• Easy-to-use PCR master mix and unique multi-enzyme KLD mix offer convenience and quality
• Rapid and direct treatment step proceeds at room temperature in 5 minutes
• Allows the use of any chemically-competent E. coli cells suitable for cloning
• Use NEBaseChanger to generate primer sequences and an annealing temperature