NEBNext® Ultra™ II Non-Directional RNA Second Strand Synthesis Module

This module is part of the non-directional Ultra™ II RNA workflow, for Illumina^®– compatible non-strand-specific RNA library construction. This workflow is compatible with poly(A) mRNA isolation or ribosomal RNA depletion, and enables high yield preparation of high quality libraries from 5 ng – 1 µg total RNA.

Kits are also available that include reagents for every step in the non-directional Ultra II RNA library construction workflow (NEBNext^® Ultra II RNA Library Prep Kit for Illumina, NEB #E7770 and NEBNext Ultra II RNA Library Prep with Sample Purification Beads, NEB #E7775).