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Application

Bond aid for polar to nonpolar substances. Compatibilizer for polymer blends.

Features and Benefits

Contributes abrasion resistance, lubricity, anti-blocking, and surface glass, allows chemical bonding via anhydride group.

Description

CAS number: 117-39-5
Chemical Formula: C15H10O7
Molar Mass: 302.24g/mol
Synonyms: 2-(3,4-Dihydroxyphenyl)-3,5,7-trihydroxy-4H-1-benzopyran-4-one, 3,3′,4′,5,6-Pentahydroxyflavone

Application

• Quercetin has been used as an antioxidant which reversed the immunosuppressive effects of high glucose and hyperglycemic sera in type 2 diabetic patients.
• It has been used as a detoxifying phytochemical in Apis mellifera.
• It has been used as a positive control in DPPH (2,2- diphenyl-1-picryhydrazyl) radical scavenging assay. It has also been used for the preparation of calibration curve to determine total flavonoid content.

Description

pH : > 7.2

Components :

HEPES: 20 mM
NaHCO3: no
sodium pyruvate: no
phenol red: yes
L-glutamine: yes

RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage-dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells. This medium is suitable for culturing human normal and neoplastic leukocytes.

RPMI-1640 medium formulation is based on the RPMI-1630 series of media utilizing a bicarbonate buffering system and alterations in the amounts of amino acids and vitamins. It is used for the culture of human normal and neoplastic leukocytes. RPMI-1640 also supports the growth of various types of cell cultures, like fresh human lymphocytes in the 72-hour phytohemagglutinin (PHA) stimulation assay.

Description:

CAS Number : 18883-66-4

Molecular weight : 265.22 g/mol

Empirical formula : C8H15N3O7

Synonyms : N-(Methylnitrosocarbamoyl)-α-D-glucosamine, Streptozotocin

Streptozocin or streptozotocin, is obtained from Streptomyces achromogenes.[1] Streptozotocin, a glucosamine-nitrosourea, is a DNA alkylating agent that enters cells exclusively via the GLUT2 glucose transport protein. Streptozocin, a diabetagen, is especially toxic to pancreatic islet insulin-producing β-cells. It is toxic to GLUT2 positive neuroendocrine tumor cells.

Description

CAS Number : 172827-25-7

Concentration : 10,000 × in DMSO

Usage : mL sufficient for 100 mini-gels

Synonyms : RNA gel dye, SYBR® RNA dye, safer gel stain

SYBR® Green II is a highly sensitive stain for post electrophoresis staining of RNA and ssDNA in agarose or polyacrylamide gels. SYBR® Green II is not selective for RNA staining but does exhibit a higher quantum yield when bound to RNA than to double stranded DNA.

Application :

-for the quantification of ribonucleic acid (RNA) in Escherichia coli[1]
-for staining 5′-ETS rRNA species separated on polyacrylamide gel prior to Northern blot analysis[2]
-to stain formaldehyde agarose gels to visualize RNA bands[3]

Description
Empirical Formula: C6H14.5NO6S · 0.5 Na
Molecular Weight: 240.24
Synonyms: 2-[(2-Hydroxy-1,1-bis[hydroxymethyl]ethyl)amino]ethanesulfonic acid, N-tris(Hydroxymethyl)methyl-2-aminoethanesulfonic acid
Assay: ≥99%
Useful pH range: 6.8 – 8.2
Application: Diagnostic assay manufacturing
Solubility: Water: 0.25 g/mL, clear, colorless

Description:

Synonyms : DNA/RNA/protein extraction reagent, DNA/RNA/protein isolation reagent, DNA/RNA/protein purification reagent, TRI Reagent® cell pellet solution, cell pellet RNA extraction, cell pellet RNA isolation, cell pellet RNA purification, single step RNA extraction reagent, single step RNA isolation reagent, single step RNA purification reagent, total RNA extraction solution, total RNA isolation solution, total RNA purification solution, TRI Reagent® RNA Isolation Reagent.

TRI reagent® is a quick and convenient ready-to-use reagent useful for efficient total RNA extraction or for the simultaneous isolation of RNA, DNA and protein. TRI reagent® is a mixture of guanidine thiocyanate and phenol in a monophasic solution.

Homogenization or lysis of the tissue sample in TRI reagent® dissolves RNA, DNA and protein. The addition of chloroform or 1-bromo-3-chloropropane followed by centrifugation results in the separation of the mixture into three phases: an aqueous phase containing the RNA, the interphase containing DNA, and an organic phase containing proteins. Each of the components can then be isolated after separating the phases.

Description

CAS number: 112-27-6
Chemical Formula: HO(CH2CH2O)2CH2CH2OH
Molar Mass: 150.17g/mol
Synonyms: Triglycol

Description

CAS number: 9005-64-5
Suitability: Suitable for blocking, cell culture, hybridization, protein purification, transformation
Synonym: Polyethylene glycol sorbitan monolaurate, Polyoxyethylenesorbitan monolaurate

General description

Tween^®20 is a polyoxyethylene sorbitol ester that belongs to the polysorbate family. It is a nonionic detergent having a molecular weight of 1,225 daltons, assuming 20 ethylene oxide units, 1 sorbitol, and 1 lauric acid as the primary fatty acid. The ethylene oxide subunits are responsible for the hydrophilic nature of the surfactant, while the hydrocarbon chains provide the hydrophobic environment. Sorbitol forms the backbone ring to which the ethylene oxide polymers are attached.

Application

TWEEN 20 is a non-ionic detergent that has been used in a variety of applications, including:

• Post-hybridization wash buffer after in situ hybridization.
• Used along with PBS (phosphate-buffered saline) as the washing buffer for ELISA and immunostaining.
• Acted as an emulsifying agent for the preparation of stable oil-in-water emulsions
• Used in pre-extraction of membranes to remove peripheral proteins (used at 2% for extraction of membrane-bound proteins).
• Acted as a blocking agent for membrane-based immunoassays at a typical concentration of 0.05%.
• Used for lysing mammalian cells at a concentration of 0.005 to 0.5%.

Description

CAS number: 9005-64-5
Suitability: Suitable for molecular biology
Synonym: Polyethylene glycol sorbitan monolaurate, Polyoxyethylenesorbitan monolaurate

General description

Tween^®20 is a polyoxyethylene sorbitol ester that belongs to the polysorbate family. It is a nonionic detergent having a molecular weight of 1,225 daltons, assuming 20 ethylene oxide units, 1 sorbitol, and 1 lauric acid as the primary fatty acid. The ethylene oxide subunits are responsible for the hydrophilic nature of the surfactant, while the hydrocarbon chains provide the hydrophobic environment. Sorbitol forms the backbone ring to which the ethylene oxide polymers are attached.

Application

TWEEN 20 is a non-ionic detergent that widely used in biochemical applications. It has been used:
• As an emulsifying agent for the preparation of stable oil-in-water emulsions
• In pre-extraction of membranes to remove peripheral proteins (used at 2% for extraction of membrane-bound proteins)
• As a blocking agent for membrane-based immunoassays at a typical concentration of 0.05%
• For lysing mammalian cells at a concentration of 0.005 to 0.5%
• Along with PBS in the dilution of antibodies in immunohistochemistry technique
• For washing cells in FISH (Fluorescence in situ hybridization) technique