Product Code | Description | Product Size | Quantity |
CM1153B | Mrs (ISO) Agar 500g | – | 500g |
MRS (ISO) Agar 500g
Brand:
Thermo ScientificTM OxoidTM
Isolate and enumerate lactic acid bacteria from food with Thermo Scientific™ Oxoid™ MRS (ISO) Agar (DeMan, Rogosa and Sharpe) (Dehydrated) while adhering to ISO 15214: 1998. This medium is designed to support the growth of all strains of Lactobacilli, even the difficult and slow-growing strains of Lactobacillus brevis and Lactobacillus fermenti.
MRS (ISO) Agar (DeMan, Rogosa and Sharpe), Oxoid Composition
Typical Formula * | gm/litre |
Enzymatic digest of casein | 10 |
Meat extract | 10 |
Yeast extract | 4 |
Tri-ammonium citrate | 2 |
Sodium acetate | 5 |
Magnesium sulphate heptahydrate | 0.2 |
Manganese sulphate tetrahydrate | 0.05 |
Di-potassium hydrogen phosphate | 2 |
Sorbitan mono-oleate | 1.08 |
Glucose | 20 |
Agar | 12.4 |
TOTAL | 66.73g/L |
MRS (ISO) Agar (DeMan, Rogosa and Sharpe), Oxoid Preparation:
Suspend 66.73g in 1 litre of distilled water. Boil to dissolve the medium completely. Dispense into tubes, bottles or flasks and sterilize by autoclaving at 121°C for 15 minutes. Selectivity can be altered by pH adjustment.
Storage conditions and Shelf life
Store the dehydrated medium at 10–30°C and use before the expiry date on the label.
Store the prepared plates at 2–8°C.
Precautions
Sterilization of this medium in some autoclaves, at 121°C for 15 minutes, may cause the medium in some cases to fall outside the specified pH limit of 5.7 ± 0.1. Adjustment of the medium in these rare cases is recommended using acetic acid or sodium hydroxide.
Dimensions | 10 cm |
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Brand | Oxoid |
Estimated Delivery Time | 1-2 weeks |