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FEATURED PRODUCTS
Haemophilus Testing Medium 500g
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Thermo ScientificTM OxoidTM
- Oxoid Haemophilus Test Medium Base (HTM) is used for the susceptibility testing of Haemophilus influenzae.
- Also available HTM Supplement, Part No. SR0158E. Store at -20° to 0°C.
Haemophilus Test Medium Base (HTM), Oxoid Composition
| Typical Formula* | gm/litre |
| Mueller-Hinton Agar | 38.0 |
| Yeast extract (specifically selected for low antagonist levels) | 5.0 |
| pH 7.3 ± 0.1 @ 25°C |
Haemophilus Test Medium (HTM), Oxoid Preparation:
Suspend 21.5g of Haemophilus Test Medium Base in 500ml of distilled water. Bring to the boil to dissolve. Sterilise by autoclaving at 121° C for 15 minutes. Cool to 50°C and aseptically add the contents of 1 vial of Haemophilus Test Medium Supplement SR0158, as directed. Mix well and pour into sterile Petri dishes.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates of medium at 2-8°C.
When stored as directed the supplement is stable until the expiry date stated on the label.
VWR Hycare Hand Sanitizer, WHO Recommended (loose pack)
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VWR
The VWR HYCARE HANDRUB is ready to use alcohol-based hand cleaner with World Health Organisation (WHO) recommended formulation.
1. Evidence-based, intrinsic advantages of fast-acting and broad-spectrum microbicidal activity with minimal risk of generating resistance to antimicrobial agents.
2. Suitability for use in resource-limited or remote areas with lack of accessibility to sinks or other facilities for hand hygiene (including clean water, towels, etc.)
3. Capacity to promote improved compliance with hand hygiene by making the process faster and more convenient.
4. Economic benefit by reducing annual costs for hand hygiene.
5. Minimization of risks from adverse events because of increased safety associated with better acceptability and tolerance than other products.
The advantages of VWR Hycare Handrub
- The microbial activity of the WHO-recommended formulations was tested by WHO reference laboratories according to EN standards (EN1500).
- Fragrance-free because of the risk of allergic reactions.
- Strict quality control and testing in a qualified laboratory to ensure the composition is met to standard and no harmful chemicals like methanol are present.
- Economy yet effective.
Formulation:
Isopropyl Alcohol | 75% v/v |
High Purity Water | 25% v/v |
Glycerol | 1.45% v/v |
Hydrogen Peroxide | 0.125% v/v |
** For more information, please read Brochure for VWR Hycare Product and COA for VWR Hycare Hand Sanitizer.
Buffered Peptone Water 2.5 kg
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Thermo ScientificTM OxoidTM
Pre-enrich food samples prior to the isolation of Salmonella species using Thermo Scientific™ Oxoid™ Buffered Peptone Water (Dehydrated). Buffered Peptone Water is a non-selective pre-enrichment medium. It also provides conditions for resuscitation of cells that have been injured by processes of food preservation.
Buffered Peptone Water, Oxoid Composition
| Typical Formula* | gm/litre |
| Peptone | 10.0 |
| Sodium chloride | 5.0 |
| Disodium phosphate | 3.5 |
| Potassium dihydrogen phosphate | 1.5 |
| pH 7.2 ± 0.2 @ 25°C |
Buffered Peptone Water, Oxoid Preparation:
Add 20g to 1 litre of distilled water. Mix well and distribute into final containers. Sterilise by autoclaving at 121°C for 15 minutes. It is extremely important that the distilled water used is of a high quality with a low mineral content/conductivity.
Storage conditions and Shelf life
Store dehydrated medium at 10-30°C and use before the expiry date of the label.
Store the prepared medium at room temperature.
n-Heptane for liquid chromatography LiChrosolv®
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Merck
Description
CAS number: 142-82-5
Chemical Formula: CH₃(CH₂)₅CH₃
Molar Mass: 100.2 g/mol
Synonyms: n-Heptane
With their high degree of UV transmittance, low particle count, low acidity and alkalinity and low evaporation residue level, LiChrosolv® solvents are ideal for reproducible separations. Since separations are normally carried out under gradient conditions in analytical HPLC, we offer solvents in “gradient grade” as well as “isocratic grade”. This enables to minimize the gradient effect of the solvent involved. Filtered by 0.2 µm filter
OneTaq 2X Master Mix with Standard Buffer
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New England Biolabs
OneTaq® DNA Polymerase is an optimized blend of Taq and Deep Vent™ DNA polymerases for use with routine and difficult PCR experiments.
- Obtain high yields across a wide range of AT / GC content
- 2X higher fidelity than Taq
- Master mix is a 2X concentrated solution containing everything needed for robust amplification
Ethyl acetate AR, 2.5L
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Chemiz
Description
Synonyms: Ethyl ester
Formula: C4H8O2
Analytical reagent grade ethyl acetate for laboratory uses in quantitative or qualitative analytical work.
Note:
AR: Analitycal Reagent Grade: Reagents for analytical purpose or research work that need high purity.
CP: Chemically Pure Grade: Reagents for regular practical in its original purity.
Ciprofloxacin 32 M.I.C.E. (50 Pack)
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Thermo ScientificTM OxoidTM
**This product is not available at the moment.
Effortlessly establish accurate Minimum Inhibitory Concentration (MIC) values manually using Thermo Scientific™ Oxoid™ Ciprofloxacin M.I.C.Evaluator Strips (M.I.C.E.™).
MEM Eagle EBSS without L-Glutamine 1L
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Lonza
Product Overview
MEM eagle (EMEM) was developed in 1959 for cultivation of HeLa and L cells.
Amino acid concentrations conform closely to the protein composition of human cells. Higher concentrations of nutrients permit longer periods between feedings. EMEM has vitamin concentrations 2-5X greater than BME and higher amino acid concentrations than BME. EMEM is suitable for culturing a broad spectrum of mammalian cells.
Content
Sh25 Spectinomycin
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Thermo ScientificTM OxoidTM
Violet Red Bile Lactose Agar 500g
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Thermo ScientificTM OxoidTM
Detect and enumerate coliform organisms in water, food and dairy products with Thermo Scientific™ Oxoid™ Violet Red Bile Agar (Dehydrated). This lactose containing selective medium has been used for the determination of the coli-aerogenes content of water, milk and other dairy products, dairy equipment, and food products. Due to the addition of selective inhibitory components, neutral red, crystal violet, and bile salts, the medium provides selectivity against non-target Gram-positive bacteria.
Violet Red Bile Agar (Dehydrated), Oxoid Composition
| Typical Formula* | gm/litre |
| Yeast extract | 3.0 |
| Peptone | 7.0 |
| Sodium chloride | 5.0 |
| Bile Salts No.3 | 1.5 |
| Lactose | 10.0 |
| Neutral red | 0.03 |
| Crystal violet | 0.002 |
| Agar | 12.0 |
| pH 7.4 ± 0.2 @ 25°C |
Violet Red Bile Agar (Dehydrated), Oxoid Preparation:
Suspend 38.5g in 1 litre of distilled water. Bring to the boil. Continue to boil for 2 minutes or for the minimum time necessary to dissolve completely and ensure that there are no remaining flecks of unmelted agar. No further sterilisation is necessary or desirable. Mix well before pouring.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C and use as freshly as possible.
Precautions
This medium is not completely specific for Enterobacteriaceae, other organisms e.g. Aeromonas and Yersinia species may give similar reactions.
The selectivity of the medium diminishes after 24 hours incubation and organisms previously suppressed may exhibit growth.
gelONE Gel Documentation System with built in touch screen
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Cleaver Scientific
The gelONE is the ultimate in simplicity, with automated acquisition through an intuitive touch screen interface. Compatible with both traditional DNA stains and safe stains, the gelONE is suitable for any lab. 5 Megapixel camera gives excellent low light sensitivity for clear resolution of closely spaced DNA bands and ensures even low levels of fluorescence are detected. The 20 x 24 cm imaging area means even large gels can be imaged with ease. Images are captured using the built in software, and saved direct to USB memory stocks for transfer to PC. The geneQUANT advanced image analysis package is included with all systems license free.
Key application includes:
DNA – Image Agarose gels stained with EtBr, SYBR stains and runSAFE
AutoRads – the gelONE™ high resolution camera makes it ideal for capturing Autorads
Visible Light – Image Coomassie stained gels, tissues, slides and films
Product Includes: gelONE Imaging System, UV Transilluminator, UV filter, software USB with gelQUANT software, power cable and Instruction manuals
Please contact us for pricing and availability.
RNase and DNAase Away
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Bio Basic
Description
This product is currently not available. Please contact us for more information.
RNase and DNAase AwayTM: DB0339 efficiently remove surface-contaminant from glasswares and plasticwares without having a residual effect on subsequent DNA & RNA samples . The product provides more effective at degradading DNA than autoclave.
OmniPur Water, WFI Quality, Sterile Purified Water, Cell Culture Tested – CAS7732-18-5
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Merck
** This product has been discontinued.
OmniPur Water is sterile water that is tested to be DNase free, Protease free, RNase free and bacterial free.
It is suitable to be used in:
- Cell Biology
- Cell culture media preparation, extraction buffer, Molecular work, reconstitute of compound
- Diluent for live cells staining
- Sterile solution preparation Microbiology Sample Preparation
- Diluent and rinsing reagent in Microbial Membrane Filtration
Key Specification:
- Sterile by USP Standard
- DNase, RNase, Protease-Free
- < 500 ppb Total Organic Carbon
- < 0.025 EU/ml Bacterial Endotoxin
Minimum Essential Medium Eagle (EMEM) with Alpha Modification, powder form
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Sigma-Aldrich
This is the most enriched variation of the EMEM formulation offered that suitable for cell culuture. It contains all 21 normal amino acids, some at increased concentrations. In addition, it contains 5 additional vitamins. Refer to the table below and choose your preferecd EMEM!
OEM SeaKem® LE Agarose
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Lonza
Product Overview
SeaKem® LE Agarose was the first and is still the world’s most trusted agarose for nucleic acid electrophoresis. Ideal for routine analysis of nucleic acids by gel electrophoresis and blotting, each SeaKem® LE Gel sharply resolves DNA and provides consistent resolution from lot-to-lot. This molecular biology grade agarose has no detectable DNase or RNase activity and forms strong gels with low background upon ethidium bromide, SYBR® Green, or GelStarTM Staining. Due to its low EEO, DNA will have a high electrophoretic mobility. SeaKem® LE Agarose is also recommended for protein gel electrophoresis in applications such as Ouchterlony and radial immunodiffusion (RID). Information about bulk and custom packaging is available through Lonza’s Customer Service Team.
Benefits
Applications
Content & Storage
1 kb DNA Ladder, NEB
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New England Biolabs
This 1 kb DNA ladder is available in Quick-Load Purple format – enjoy the convenience of a ready-to-load format.
1 kb DNA ladder from New England Biolabs (NEB) is suitable for use with molecular weight standard for agarose gel electrophoresis. A number of proprietary plasmids are digested to completion with appropriate restriction enzymes to yield 10 bands, ranging from 0.5 to 10 kilobases. NEB set 3.0 kb fragment as a reference band by increasing the intensity of that band.
- Size range: 500 bp to 10 kb
- Value pricing
- Supplied with a free vial of Gel Loading Dye, Purple (6X), no SDS (NEB #B7025).
- Easy to identify reference bands
- Small size suitable for 200 gel lanes; large size suitable for 1000 gel lanes
Q5 Hot Start High-Fidelity 2X Master Mix
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New England Biolabs
Fidelity at its finest
Q5® High-Fidelity DNA Polymerase sets a new standard for both fidelity and robust performance. With the highest fidelity amplification available (~280 times higher than Taq), Q5 DNA Polymerase results in ultra-low error rates. Q5 DNA Polymerase is composed of a novel polymerase that is fused to the processivity-enhancing Sso7d DNA binding domain, improving speed, fidelity and reliability of performance.
- Highest fidelity amplification (~280X higher than Taq)
- Ultra-low error rates
- Superior performance for a broad range of amplicons (from high AT to high GC)
- Hot start and master mix formats available
FEATURED PRODUCTS
Instant Sticky-end Ligase Master Mix
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New England Biolabs
Specifically formulated to rapidly ligate cohesive-end (2–4 bp) substrates and improve transformation, Instant Sticky-end Ligase Master Mix is a ready-to-use 2X solution of T4 DNA Ligase and a proprietary ligation enhancer in an optimized reaction buffer.
- No incubation time necessary to achieve ligation
- Efficiencies sufficient for successful sub-cloning of sticky-end substrates
- No thawing of the master mix is required as liquid state is maintained when stored at -20°C
- Simplified reaction setup with optimized ratio of enzyme and buffer components
- Ligations for subcloning can be carried out in small volumes with low DNA concentrations, allowing users to conserve precious DNA samples and directly transform many strains of chemically competent E. coli without dilution
- Not sure which ligase to choose? Refer to our DNA and RNA Ligase Properties Chart
Yeast Autolysate Supplement 1 x 10 Vials
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Thermo ScientificTM OxoidTM
Oxoid Yeast Autolysate Supplement is used for the preparation of New York City Medium for the isolation of Neisseria spp.
- Add to GC Agar Base, Part No. CM0367B
- Each vial supplements 500mL of medium
Yeast Autolysate Supplement, Oxoid Composition
| Vial contents (each vial is sufficient for 500ml of medium) | |
| Yeast autolysate fractions | 5.0 g |
| Glucose | 0.5 g |
| Sodium bicarbonate | 0.075 g |
Yeast Autolysate Supplement, Oxoid Preparation:
Reconstitute one vial as directed, aseptically add the contents to 500 ml of sterile GC Agar Base CM0367, 1% (w/v) Soluble Haemoglobin Powder LP0053. Mix gently and pour into sterile Petri dishes.
PowerPro 300
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Cleaver Scientific
The PowerPro 300 is a versatile multi-function power supply suitable for a wide range of electrophoresis applications. At 300V, 700mA and 150W the PowerPro 300 has nearly twice the current and power of the market leading equivalent unit. Perfectly suited for use with Cleaver Scientific’s multiSUB range of horizontal electrophoresis systems and omniPAGE Mini vertical electrophoresis systems. The PowerPro 300 even has built in pre-set protocols from running each of Cleaver Scientific’s electrophoresis systems, and allows users to create and save protocols for their own systems.
The PowerPro 300 is the ideal power supply for:
- Routine horizontal electrophoresis using multiSUB Mini, Midi and Choice.
- Vertical Electrophoresis using omniPAGE Mini.
Q5® High-Fidelity DNA Polymerase, NEB
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New England Biolabs
Q5® High-Fidelity DNA Polymerase, NEB sets a new standard for both fidelity and robust performance. With the highest-fidelity amplification available (~280 times higher than Taq), Q5 DNA Polymerase results in ultra-low error rates. Q5 DNA Polymerase is composed of a novel polymerase that is fused to the processivity-enhancing Sso7d DNA binding domain, improving speed, fidelity and reliability of performance.
Features of Q5® High-Fidelity DNA Polymerase, NEB:
- Highest fidelity amplification (~280X higher than Taq)
- Ultra-low error rates
- Superior performance for a broad range of amplicons (from high AT to high GC)
- Hot start and master mix formats available
TWEEN® 20 for Molecular Biology (Sigma-Aldrich)
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Sigma-Aldrich
Description
CAS number: 9005-64-5
Suitability: Suitable for molecular biology
Synonym: Polyethylene glycol sorbitan monolaurate, Polyoxyethylenesorbitan monolaurate
General description
Tween®20 is a polyoxyethylene sorbitol ester that belongs to the polysorbate family. It is a nonionic detergent having a molecular weight of 1,225 daltons, assuming 20 ethylene oxide units, 1 sorbitol, and 1 lauric acid as the primary fatty acid. The ethylene oxide subunits are responsible for the hydrophilic nature of the surfactant, while the hydrocarbon chains provide the hydrophobic environment. Sorbitol forms the backbone ring to which the ethylene oxide polymers are attached.
Application
TWEEN 20 is a non-ionic detergent that widely used in biochemical applications. It has been used:
• As an emulsifying agent for the preparation of stable oil-in-water emulsions
• In pre-extraction of membranes to remove peripheral proteins (used at 2% for extraction of membrane-bound proteins)
• As a blocking agent for membrane-based immunoassays at a typical concentration of 0.05%
• For lysing mammalian cells at a concentration of 0.005 to 0.5%
• Along with PBS in the dilution of antibodies in immunohistochemistry technique
• For washing cells in FISH (Fluorescence in situ hybridization) technique
W2.5 Trimethoprim
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Thermo ScientificTM OxoidTM
Amoxycillin 256 M.I.C.E. (10 Pack)
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Thermo ScientificTM OxoidTM
**This product is not available at the moment.
Effortlessly establish accurate Minimum Inhibitory Concentration (MIC) values manually using Thermo Scientific™ Oxoid™ Amoxycillin M.I.C.Evaluator Strips (M.I.C.E.™).
GelStarTM Nucleic Acid Gel Stain, 10,000X (2 x 250 uL)
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Lonza
GelStarTM Nucleic Acid Gel Stain is a highly sensitive fluorescent stain for detecting both DNA and RNA. You can choose to either add GelStarTM Stain to your agarose solution prior to casting or to post stain your gels. GelStarTM Nucleic Acid Gel Stain exhibits exceptional signal-to-noise ratio with minimal background. Maximum sensitivity, detect as little as 20 pg of dsDNA or 3 ng of RNA.
Benefits
Applications
Lab-Lemco Powder (Beef Extract)
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Thermo ScientificTM OxoidTM
Thermo Scientific™ Oxoid Lab-Lemco Powder is a Beef Extract made from specially selected raw materials.
- May be used in fermentation processes
- Industrial Reference: NMKL
Magnesium Chloride (MgCl2) Solution – 6.0 ml
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New England Biolabs
25 mM MgCl2 for use in conjunction with polymerase reaction buffers.
- MgCl2 is available for use in conjunction with NEB’s polymerase reaction buffers
- Supplied as 4 x 1.5 ml vials
Zinc Oxide, CP, 500g
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Chemiz
Description
Formula: ZnO
Zinc oxide has a variety of uses including rubber and ceramic glaze manufacture, UV absorber, paint corrosion inhibitor and as nutrient supplement.
Note:
AR: Analitycal Reagent Grade: Reagents for analytical purpose or research work that need high purity.
CP: Chemically Pure Grade: Reagents for regular practical in its original purity.
Vancomycin Supplement (5mg)
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Thermo ScientificTM OxoidTM
Thermo Scientific™ Oxoid Vancomycin Supplement is for addition to selective media for the isolation of Vancomycin Resistant Enterococci (VRE) and High Level Aminoglycoside Resistant Enterococci (HLARE) from clinical specimens.
- Each vial supplements 500mL of medium
Vancomycin Supplement, Oxoid Composition
| Vial contents: (each vial sufficient for 500ml of medium) | per vial | per litre |
| Vancomycin | 5mg | 10mg |
Cronobacter Screening Broth (CSB), Oxoid Preparation:
Dissolve 14.02g in 500ml of distilled water. Sterilize by autoclaving at 121°C for 15 minutes. Cool to approximately 50°C, and aseptically add the contents of 1 vial of Vancomycin Supplement (5mg) (SR0247E) reconstituted as directed. Mix well and aseptically dispense into sterile containers.
Modified Lauryl Sulphate Tryptose Broth, Oxoid Preparation:
Dissolve 32.3g in 500ml of distilled water. Sterilize by autoclaving at 121°C for 15 minutes. Cool to approximately 50°C, and aseptically add the contents of 1 vial of Vancomycin Supplement (5mg) (SR0247E) reconstituted as directed. Mix well and aseptically dispense into sterile containers.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
OmniPur Water, WFI Quality, Sterile Purified Water, Cell Culture Tested – CAS7732-18-5
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Merck
** This product has been discontinued.
OmniPur Water is sterile water that is tested to be DNase free, Protease free, RNase free and bacterial free.
It is suitable to be used in:
- Cell Biology
- Cell culture media preparation, extraction buffer, Molecular work, reconstitute of compound
- Diluent for live cells staining
- Sterile solution preparation Microbiology Sample Preparation
- Diluent and rinsing reagent in Microbial Membrane Filtration
Key Specification:
- Sterile by USP Standard
- DNase, RNase, Protease-Free
- < 500 ppb Total Organic Carbon
- < 0.025 EU/ml Bacterial Endotoxin
Minimum Essential Medium Eagle (EMEM) with Alpha Modification, powder form
Login to see pricesBrand:
Sigma-Aldrich
This is the most enriched variation of the EMEM formulation offered that suitable for cell culuture. It contains all 21 normal amino acids, some at increased concentrations. In addition, it contains 5 additional vitamins. Refer to the table below and choose your preferecd EMEM!
OEM SeaKem® LE Agarose
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Lonza
Product Overview
SeaKem® LE Agarose was the first and is still the world’s most trusted agarose for nucleic acid electrophoresis. Ideal for routine analysis of nucleic acids by gel electrophoresis and blotting, each SeaKem® LE Gel sharply resolves DNA and provides consistent resolution from lot-to-lot. This molecular biology grade agarose has no detectable DNase or RNase activity and forms strong gels with low background upon ethidium bromide, SYBR® Green, or GelStarTM Staining. Due to its low EEO, DNA will have a high electrophoretic mobility. SeaKem® LE Agarose is also recommended for protein gel electrophoresis in applications such as Ouchterlony and radial immunodiffusion (RID). Information about bulk and custom packaging is available through Lonza’s Customer Service Team.
Benefits
Applications
Content & Storage
1 kb DNA Ladder, NEB
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New England Biolabs
This 1 kb DNA ladder is available in Quick-Load Purple format – enjoy the convenience of a ready-to-load format.
1 kb DNA ladder from New England Biolabs (NEB) is suitable for use with molecular weight standard for agarose gel electrophoresis. A number of proprietary plasmids are digested to completion with appropriate restriction enzymes to yield 10 bands, ranging from 0.5 to 10 kilobases. NEB set 3.0 kb fragment as a reference band by increasing the intensity of that band.
- Size range: 500 bp to 10 kb
- Value pricing
- Supplied with a free vial of Gel Loading Dye, Purple (6X), no SDS (NEB #B7025).
- Easy to identify reference bands
- Small size suitable for 200 gel lanes; large size suitable for 1000 gel lanes
Q5 Hot Start High-Fidelity 2X Master Mix
Login to see pricesBrand:
New England Biolabs
Fidelity at its finest
Q5® High-Fidelity DNA Polymerase sets a new standard for both fidelity and robust performance. With the highest fidelity amplification available (~280 times higher than Taq), Q5 DNA Polymerase results in ultra-low error rates. Q5 DNA Polymerase is composed of a novel polymerase that is fused to the processivity-enhancing Sso7d DNA binding domain, improving speed, fidelity and reliability of performance.
- Highest fidelity amplification (~280X higher than Taq)
- Ultra-low error rates
- Superior performance for a broad range of amplicons (from high AT to high GC)
- Hot start and master mix formats available
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