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• Oxoid Membrane Clostridium Perfringens Agar Base (m-CP Agar Base) is a chromogenic medium for rapid isolation and presumptive identification of Clostridium perfringens from water samples.
• Also available: Membrane Clostridium Perfringens Selective Supplement, Part No. SR0188E.

Membrane Clostridium Perfringens (M-CP) Agar Base, Oxoid Composition

(M-CP) Agar Base, Oxoid Preparation:

Suspend 35.55g of m-CP Agar Base in 500ml of distilled water Mix well and sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C and aseptically add the contents of one vial of m-CP Selective Supplement reconstituted as directed. Aseptically add the following sterile solutions dissolved in distilled water:

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Prepared medium may be stored for up to 5 days at 2-8°C in the dark.

Agar Bacteriological (Agar No. 1, Oxoid) is a clear purified high gel strength bacteriological agar compatible with all culture media. This agar has low calcium (Ca) and magnesium (Mg) levels and has very high working gel strength (1.5% w/v).

Agar Bacteriological, Oxoid is compatible with all culture media. It can be formulated to either broth or agar of the same medium with very similar metal values. This characteristic is especially valuable in antimicrobial MIC studies where differences in mineral/metal content can profoundly influence the results. Due to the low mineral/metal content in Agar Bacteriological, Oxoid, it allows free diffusion of antimicrobial substances. As a result, it is a highly satisfactory agar for antimicrobial diffusion studies (disc diffusion susceptibility tests).

This product has been discontinued. The alternative to this product is Peptone Water.

Oxoid Peptone Water Andrade’s is a nutrient base containing Andrade’s indicator to which carbohydrates and other diagnostic reagents may be added for use in fermentation studies.

Peptone Water Andrade’s, Oxoid Composition

Peptone Water Andrade’s, Oxoid Preparation:
Suspend 15 g in 1 litre of distilled water. Mix well to dissolve, distribute into tubes or bottles, containing Durham tubes and sterilize by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium in the dark at room temperature.

This product has been discontinued. Please contact us for more information.

Oxoid GBS Agar Base (Group B Streptococci Medium) (Islam) is used for the isolation and detection of Group B Streptococci in clinical specimens.

GBS Agar Base (Islam), Oxoid Composition

GBS Agar Base (Group B Streptococci Medium) (Islam), Oxoid Preparation:
Suspend 45.2g in 1 litre of distilled water and bring to the boil to disolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C and aseptically add 50ml of sterile inactivated Horse Serum. Mix well and pour into Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates at 2-8°C away from light.

Precautions
The medium must be at its correct pH value to ensure good pigmentation.
Some strains of Group B streptococci do not produce pigmented colonies.
Do not hold the molten medium any longer than necessary.

Oxoid Gelatin Bacteriological is a special bacteriological grade readily soluble in water to give a clear solution free from sulphite and preservatives.

• Firm gel obtained from 15% solution

Nutrient agar (500g, Oxoid) is a culture media that used to cultivate and isolate fastidious pathogens and other microorganisms. This non-selective medium contains beef extracts combined with peptone, yeast extracts and sodium chloride to provide the nitrogen compounds, carbon, vitamins and some trace ingredients necessary for the growth of bacteria. A general-purpose medium which may be enriched with up to 10% blood or other biological fluid.

Nutrient Agar, Oxoid Composition

Nutrient Agar, Oxoid Preparation:

Powder: Suspend 28g of nutrient agar in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

This product has been discontinued. Please contact us for more information.

Oxoid ORSAB Selective Supplement (Oxacillin Resistance Screening Agar Base Selective Supplement) is added to Oxacillin Resistance Screening Agar Base, Part No. CM1008B.

• Add to Oxacillin Resistance Screening Agar Base, Part No. CM1008B
• Each vial supplements 500mL of medium

ORSAB Selective Supplement, Oxoid Composition

ORSAB Selective Medium Preparation:
Suspend 51.75g of Oxacillin Resistance Screening Agar Base in 500ml of distilled water and bring gently to the boil to dissolve. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C and aseptically add the contents of one vial of ORSAB Selective Supplement SR0195, reconstituted as directed below. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
ORSAB Selective Supplement should be stored at 2-8°C.
Store the prepared medium at 2-8°C.

This product has been discontinued. Please contact us for more information.

• Oxoid Oxacillin Resistance Screening Agar Base (ORSAB) is a medium for screening clinical samples for the presence of oxacillin-resistant Staphylococci.
• Also available ORSAB Selective Supplement, Part No. SR0195E.

Oxacillin Resistance Screening Agar Base (ORSAB), Oxoid Composition

Oxacillin Resistance Screening Agar, Oxoid Preparation:
Suspend 51.75g of Oxacillin Resistance Screening Agar Base in 500ml of distilled water and bring gently to the boil to dissolve. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C and aseptically add the contents of one vial of ORSAB Selective Supplement SR0195, reconstituted as directed below. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Peptone water (CM0009B), Oxoid, is a basal medium that used as a growth medium or as the basis of carbohydrate fermentation media. A pure culture in Peptone Water is a convenient inoculum for a series of fermentation tubes or other diagnostic media.

Directions

Add 15g to 1 litre of distilled water. Mix well and distribute into final containers. Sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium below 25°C.

Phosphate Buffered Saline Tablets, Dulbecco A (PBS, Oxoid) is used for the preparation of a balanced salt solution without calcium and magnesium. PBS is normally used to rinse the cells free from growth medium before application of the chelating agent, such as Versene (diamino-ethane-tetra-acetic acid).

Content of Phosphate Buffered Saline, Oxoid:

Preparation of PBS Buffer from PBS tablets, Oxoid
Dissolve ONE tablet in 100ml of distilled water. Sterilize the PBS solution with autoclaving for 115°C for 15 mins. The tablets should be dissolving completely and free from insoluble matter.

Isolate and enumerate yeasts and molds from dairy and food products and prepare Aspergillus niger for the Harmonised Microbial Limit Tests from international pharmacopoeia with Thermo Scientific™ Oxoid™ Potato Dextrose Agar (Dehydrated). This medium is suitable for the detection and enumeration of heat resistant molds in thermally processed fruits and fruit products.

Pigment production is a critical part of the identification of the fungus. The agar used in the medium is carefully screened to ensure correct pigment production by fungi such as Fusaria spp.

Composition of Potato Dextrose Agar

Preparation of Potato Dextrose Agar, Oxoid
Suspend 39g in 1 litre of purified water and heat up to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Ensure the solution is well mixed before pouring.

To suppress the growth of bacteria, you can acidify the medium to pH 3.5 by adding 1 ml of lactic acid 10% SR0021 to each sterilized medium at 50°C. Note that the medium must not be heated after the addition of acid as it will destroy the gelling properties and results in hydrolysis of agar.

Storage Conditions and Shelf Life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

This product is temporarily unavailable. Please contact us for more information.

Oxoid Saline Tablets is used for preparation of 500mL of 0.85% isotonic saline. For Laboratory Use Only.