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• Oxoid Membrane Clostridium Perfringens Agar Base (m-CP Agar Base) is a chromogenic medium for rapid isolation and presumptive identification of Clostridium perfringens from water samples.
• Also available: Membrane Clostridium Perfringens Selective Supplement, Part No. SR0188E.

Membrane Clostridium Perfringens (M-CP) Agar Base, Oxoid Composition

(M-CP) Agar Base, Oxoid Preparation:

Suspend 35.55g of m-CP Agar Base in 500ml of distilled water Mix well and sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C and aseptically add the contents of one vial of m-CP Selective Supplement reconstituted as directed. Aseptically add the following sterile solutions dissolved in distilled water:

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Prepared medium may be stored for up to 5 days at 2-8°C in the dark.

Agar Bacteriological (Agar No. 1, Oxoid) is a clear purified high gel strength bacteriological agar compatible with all culture media. This agar has low calcium (Ca) and magnesium (Mg) levels and has very high working gel strength (1.5% w/v).

Agar Bacteriological, Oxoid is compatible with all culture media. It can be formulated to either broth or agar of the same medium with very similar metal values. This characteristic is especially valuable in antimicrobial MIC studies where differences in mineral/metal content can profoundly influence the results. Due to the low mineral/metal content in Agar Bacteriological, Oxoid, it allows free diffusion of antimicrobial substances. As a result, it is a highly satisfactory agar for antimicrobial diffusion studies (disc diffusion susceptibility tests).

This product has been discontinued. The alternative to this product is Peptone Water.

Oxoid Peptone Water Andrade’s is a nutrient base containing Andrade’s indicator to which carbohydrates and other diagnostic reagents may be added for use in fermentation studies.

Peptone Water Andrade’s, Oxoid Composition

Peptone Water Andrade’s, Oxoid Preparation:
Suspend 15 g in 1 litre of distilled water. Mix well to dissolve, distribute into tubes or bottles, containing Durham tubes and sterilize by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium in the dark at room temperature.

This product has been discontinued, please refer to SR0258E

Oxoid Brilliance Listeria Differential Supplement is added to Brilliance Listeria Agar Base, Mfr. No. CM1080E and Mfr. No. CM1080B, a chromogenic medium for the isolation and differentiation of Listeria

• Add to Brilliance Listeria Agar Base, Part No. CM1080B
• Each vial supplements 500mL of medium

Oxoid Brilliance Listeria Differential Supplement, Oxoid Composition

Brilliance Listeria Agar, Oxoid Preparation:
Suspend 33.6g of Brilliance Listeria Agar Base (CM1080) in 480ml of distilled water. Mix well and sterilize by autoclaving at 121°C for 15 minutes. Cool the medium to 46°C and add one vial of Brilliance Listeria Selective Supplement, reconstituted as directed and one vial of Brilliance Listeria Differential Supplement. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
The dehydrated medium should be stored at 10-30°C and used before the expiry date on the label.
Prepared medium may be stored for up to 2 weeks at 2-8°C.

This product has been discontinued, please refer to SR0257E

Brilliance Listeria Selective Supplement is added to Brilliance Listeria Agar Base, Mfr. No. CM1080E and Mfr. No. CM1080B, a chromogenic medium for the isolation and differentiation of Listeria spp. in food samples.

• Add to Brilliance Listeria Selective Agar Base, Part No. CM1080B
• Each vial supplements 500mL of medium

Brilliance Listeria Selective Supplement, Oxoid Composition

Brilliance Listeria Agar, Oxoid Preparation:
Suspend 33.6g of Brilliance Listeria Agar Base (CM1080) in 480ml of distilled water. Mix well and sterilize by autoclaving at 121°C for 15 minutes. Cool the medium to 46°C and add one vial of Brilliance Listeria Selective Supplement, reconstituted as directed and one vial of Brilliance Listeria Differential Supplement. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
The dehydrated medium should be stored at 10-30°C and used before the expiry date on the label.
Prepared medium may be stored for up to 2 weeks at 2-8°C.

This product has been discontinued, please refer to CM1212B
Brilliance Listeria Agar Base is a chromogenic medium for the isolation, enumeration, and presumptive identification of Listeria spp., including Listeria monocytogenes. Also available Chromogenic Listeria Selective Supplement, Part No. SR0227E and Chromogenic Listeria Differential Supplement, Part No. SR0228E.

Brilliance Listeria Agar Base, Oxoid Composition

Brilliance Listeria Agar, Oxoid Preparation:
Suspend 33.6g of Brilliance Listeria Agar Base (CM1080) in 480ml of distilled water. Mix well and sterilize by autoclaving at 121°C for 15 minutes. Cool the medium to 46°C and add one vial of Brilliance Listeria Selective Supplement, reconstituted as directed and one vial of Brilliance Listeria Differential Supplement. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
The dehydrated medium should be stored at 10-30°C and used before the expiry date on the label.
Prepared medium may be stored for up to 2 weeks at 2-8°C.

Thermo Scientific™ Oxoid Brilliance™ UTI Agar (formerly Chomogenic UTI Agar ) is a chromogenic medium for the presumptive identification and differentiation of all the main micro-organisms that cause urinary tract infections (UTIs).

Brilliance UTI Agar, Oxoid Composition

Brilliance UTI Agar, Oxoid Preparation:
Suspend 56.3g of Brilliance UTI Agar in 1 litre of distilled water, mix well and sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C and mix well before pouring plates.

Storage conditions and Shelf life
Dehydrated Brilliance UTI Agar must be stored tightly capped in the original container at 10-30ºC.
Oxoid Brilliance UTI Agar plates should be stored in the original packaging, at the temperature stated on the pack or product specification, and protected from direct light.

When stored as directed, the unopened product will remain stable until the expiry date on the label. Locally prepared media can be stored for up to 2 weeks when made from CM0949 according to the manufacturer’s instructions and stored at 2-8ºC, in the dark. A longer shelf life may be attainable, but should be validated under the relevant, local manufacturing and storage conditions.

This product has been discontinued, please refer to CM1212B

Isolate, enumerate and differentiate Listeria species and Listeria monocytogenes from food samples with Thermo Scientific™ Oxoid™ Chromogenic Listeria Agar (ISO) (Dehydrated). Chromogenic Listeria Agar (ISO) contains lithium chloride, polymyxin B, nalidixic acid, and amphotericin which act to inhibit non-target organisms. A combination of chromogenic and phospholipase enzyme reactions easily differentiate Listeria monocytogenes (blue colonies surrounded by an opaque halo) from other Listeria spp. (blue colonies without an opaque halo).

Chromogenic Listeria Agar (ISO), Oxoid Composition

Modified ISO Preparation:
Suspend 34.5g of Chromogenic Listeria Agar (ISO) in 480ml of distilled water. Mix well and sterilize by autoclaving at 121°C for 15 minutes. Cool the medium to 46°C and add one vial of Chromogenic Listeria Selective Supplement (ISO), reconstituted as directed and one vial of Brilliance Listeria Differential Supplement. Mix well and pour into sterile Petri dishes.

ISO Preparation:
Suspend 34.5g of Chromogenic Listeria Agar (ISO) Base in 480ml of distilled water. Mix well and sterilise by autoclaving at 121ºC for 15 minutes. Cool the medium to around 50ºC, and add one vial of Chromogenic Listeria Selective Supplement (ISO), re-suspended as directed, and one vial of OCLA (ISO) Differential Supplement. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
The dehydrated medium should be stored at 10-30°C and used before the expiry date on the label.
Prepared medium may be stored for up to 2 weeks at 2-8°C.

This product has been discontinued. Please contact us for more information.

Oxoid GBS Agar Base (Group B Streptococci Medium) (Islam) is used for the isolation and detection of Group B Streptococci in clinical specimens.

GBS Agar Base (Islam), Oxoid Composition

GBS Agar Base (Group B Streptococci Medium) (Islam), Oxoid Preparation:
Suspend 45.2g in 1 litre of distilled water and bring to the boil to disolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C and aseptically add 50ml of sterile inactivated Horse Serum. Mix well and pour into Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates at 2-8°C away from light.

Precautions
The medium must be at its correct pH value to ensure good pigmentation.
Some strains of Group B streptococci do not produce pigmented colonies.
Do not hold the molten medium any longer than necessary.

Oxoid Gelatin Bacteriological is a special bacteriological grade readily soluble in water to give a clear solution free from sulphite and preservatives.

• Firm gel obtained from 15% solution

Oxoid Hemoglobin Powder Soluble is a specially prepared hemoglobin for use in culture media.

Thermo Scientific™ Oxoid Legionella BMPA-α Selective Supplement is a selective supplement for the isolation of legionellae when used with Legionella BCYE Growth Supplement (SR0010) and Legionella CYE Agar Base (CM0655).

Legionella BMPA-α Selective Supplement, Oxoid Composition

Legionella BMPA-α Selective Medium Preparation:
Reconstitute one vial SR0111E per 100ml medium as directed. Aseptically add the vial contents to BCYE-a Medium prepared from Legionella CYE Agar Base (CM0655) and Legionella BCYE Supplement (SR0110) and cooled to 50°C. Mix well and pour into sterile Petri dishes.

MacConkey agar, Oxoid is used to detect, isolate and enumerate coliforms and intestinal pathogens in water, dairy products and biological specimens. This differential medium gives a number of diagnostic indications such as bile tolerance, colony morphology and chromogenesis. MacConkey Agar corresponds to the medium recommended by the World Health Organizaton, the Department of Health for the bacterial examination of water.

Composition of MacConkey Agar (Oxoid)

Preparation of MacConkey Agar (Oxoid):

Suspend 52g of MacConkey agar powder in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life of MacConkey Agar (Oxoid)
Store the dehydrated MacConkey agar powder at 10-30°C and use before the expiry date on the label.
Store the prepared MacConkey agar at 2-8°C.

Presumptively detect, isolate and enumerate pathogenic staphylococci in milk, food, fish and other specimens with Thermo Scientific™ Oxoid™ Mannitol Salt Agar (Dehydrated). Due to high concentrations of salt in the medium, most unwanted bacteria are inhibited with the exception of some halophilic marine organisms. Presumptive coagulase-positive staphylococci produce colonies surrounded by bright yellow zones while non-pathogenic staphylococci produce colonies with reddish purple zones.

Mannitol Salt Agar, Oxoid Composition

Mannitol Salt Agar, Oxoid Preparation:
Suspend 111g in 1 litre of distilled water and bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates at 2-8°C.

Perform effortless Antimicrobial Susceptibility Testing (AST) using Thermo Scientific™ Oxoid™ Mueller Hinton Agar (Dehydrated), a medium designed and manufactured according to the European standard, EUCAST and the American standard, CLSI.

Composition of Mueller Hinton Agar, Oxoid

Preparation of Mueller Hinton Agar, Oxoid
Add 38g of Mueller Hinton Agar to 1 litre of distilled water. Bring to the boil to dissolve the medium completely. Sterilize by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life:
Store the dehydrated Mueller Hinton Agar, Oxoid at 10-30°C and use before the expiry date on the label.
Store the prepared Mueller Hinton Agar plates at 2-8°C.

Precautions
Incubation in a carbon dioxide enriched atmosphere is not recommended because of its pH effect on the medium. If it is imperative to use CO₂ then known control organisms should be included with the test plates to measure its effect.
Carbohydrates should not be added to Mueller-Hinton Agar because they may influence the rate of growth of the organism and the resulting pH of the medium.
The addition of lysed horse blood to the medium may further reduce the levels of thymidine and prevent the growth of thymidine-dependent organisms.

The medium may not support the growth of some fastidious strains for example microorganisms that require thymine and thymidine for growth. Close adherence to the methodology is required to obtain satisfactory results.