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FEATURED PRODUCTS
Monarch® Total RNA Miniprep Kit
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New England Biolabs
Quickly and easily purify up to 100 µg of high quality total RNA from multiple sample types – all with Monarch Total RNA Miniprep kit!
- For use with blood, cells and tissues
- Also works with tough to lyse samples (bacteria, yeast, plant)
- Effectively purifies total RNA of all sizes, including small RNAs >20 nt
- Efficient genomic DNA removal (column and DNase I-based)
- Contains Proteinase K for processing of tissues and blood samples
- Includes RNA Protection Reagent for sample preservation
- Excellent value
- Kit components available separately
** You may also interest in RNA Purification from Buccal Swabs, Nasopharyngeal Samples (swab or aspirate) and Saliva using the Monarch Total RNA Miniprep Kit.
Amicon® Ultra Filter
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Merck
Description
Applications Include:
Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms.
Purification of macromolecular components found in tissue culture extracts or cell lysates.
Primer removal, PCR cleanup, removal of linkers or macromolecular labels from a reaction mix, and protein removal prior to HPLC.
Desalting, buffer exchange and protein dialysis.
Schaedler Agar 500g
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Thermo ScientificTM OxoidTM
Grow and isolate aerobic and anaerobic organisms of the intestinal tract, which may require selective conditions to inhibit the growth of background organisms like Escherichia coli, with Thermo Scientific™ Oxoid™ Schaedler Anaerobe Agar. The presence of cysteine in the medium provides selectivity against Escherichia coli as cysteine has shown to have inhibitory effects on several enzymatic reactions of Escherichia coli in vitro.1
Schaedler Anaerobe Agar, Oxoid Composition
| Typical Formula* | gm/litre |
| Tryptone Soya Broth (Oxoid CM129) | 10.0 |
| Special peptone | 5.0 |
| Yeast extract | 5.0 |
| Glucose | 5.0 |
| Cysteine HCl | 0.4 |
| Haemin | 0.01 |
| Tris Buffer | 0.75 |
| Agar | 13.5 |
| pH 7.6 ± 0.2 @ 25°C |
Schaedler Anaerobe Agar, Oxoid Preparation:
Suspend 40g in 1 litre of distilled water and boil to dissolve the medium completely. Sterilise by autoclaving at 121°C for 15 minutes. Mix well before pouring.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Prepared plates may be stored at 2-8°C if suitably protected.
Cytochrome C from Bovine Heart (Sigma-Aldrich)
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Sigma-Aldrich
Description
CAS number: 9007-43-6
Application
Cytochrome c has been identified as an important mediator in apoptotic pathways. The release of mitochondrial cytochrome c into the cytoplasm stimulates apoptosis and is commonly used as an indicator of the apoptotic process in the cell. Investigation on the effect of Paris Saponin I (PS I) on human gastric carcinoma cell growth (SGC7901 cells) have shown an elevated level cytoplasmic cytochrome c. Results are inhibition of proliferation in SGC7901 cells by inducing mitochondria-dependent apoptosis through cytochrome c.
Biochem/physiol Actions
The ready interconversion of cytochrome c between ferrous and ferric states makes it an efficient biological electron carrier. It plays a vital role in cellular oxidations in both plants and animals. Generally regarded as a universal link in the respiratory chain, it forms the essential electron-bridge between the respirable substrates and oxygen.
V.C.N. Selective Supplement 1 x 10 Vials
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Thermo ScientificTM OxoidTM
This product has been discontinued. Please contact us for more information.
Oxoid VCN Selective Supplement is used for the preparation of Thayer Martin Medium for the isolation of pathogenic Neisseria spp.
- Add to GC Agar Base, Part No. CM0367B
- Each vial supplements 500mL of medium
VCN Selective Supplement, Oxoid Composition
| Vial contents (each vial is sufficient for 500ml of medium) | |
| Vancomycin | 1.5 mg |
| Colistin sulphate | 3.75 mg |
| Nystatin | 6,250 IU |
VCN Selective Medium Preparation:
Reconstitute one vial as directed, aseptically add the contents to 500 ml of sterile Thayer Martin Medium, cooled to 50°C, prepared from GC Agar Base CM0367, 1% (w/v) Soluble Haemoglobin Powder LP0053 plus the rehydrated contents of one vial of Vitox SR0090. Mix gently and pour into sterile Petri dishes.
Cfp30 Cefoperazone
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Thermo ScientificTM OxoidTM
McFarland Turb Standard 0.5
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Thermo Scientific™ Remel™
Thermo Scientific™ Remel McFarland Equivalence Turbidity Standard is packaged in plastic case with a visual comparison card.
- Tube size is 15 x 103mm and fits in most spectrophotometers
- Polystyrene technology allows longer shelf life
Complete Mini Wide Vertical Electrophoresis Modular System
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Cleaver Scientific
The omniPAGE range of Modular Vertical Gel Systems allow multiple electrophoresis techniques to be performed in the same unit. These systems include all modules and accessories required for Slab Gel Electrophoresis, 2-D Electrophoresis and Electroblotting. The central component is the omniPAGE Mini Vertical unit, Mini Wide Vertical Unit or omniPAGE Maxi Vertical unit.
Package includes:
- 1x omniPAGE Mini Wide Vertical Unit which includes: 2x4mm thick notched glass plates, 2x4mm thick plain glass plates with 1mm thick bonded spacers, 1x dummy plate, 2x combs (1mm thick, 24 samples), 1x casting base, silicone mat, cooling pack
- 1x Capillary Electrophoresis Module which includes: internal running module for tube gels, capillary tubes, blanking plugs, 2-D combs and spacers
- 1x Electroblotting Module comprising: internal electroblotting module, 4x compression cassettes for gel sizes up to 20x10cm and 6x fibre pads.
TBX Medium 500g
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Thermo ScientificTM OxoidTM
Detect and enumerate Escherichia coli in food and animal feed samples with Thermo Scientific™ Oxoid™ Typtone Bile X-Glucuronide (TBX) Medium (Dehydrated). The medium meets the formulation and performance criterion laid down in ISO 16649 and ISO 11133:2014.
TBX Medium builds on the speed, reliability and recovery from frozen samples and detection of poor lactose fermenters offered by the original Tryptone Bile Agar, with the addition of a chromogenic agent, X-glucuronide, which detects glucuronidase activity, an enzyme shown to be highly specific for Escherichia coli.
Typtone Bile X-Glucuronide (TBX) Medium (Dehydrated), Oxoid Composition
| Typical Formula* | gm/litre |
| Tryptone | 20.0 |
| Bile Salts No. 3 | 1.5 |
| Agar | 15.0 |
| X-glucuronide | 0.075 |
| pH 7.2 ± 0.2 @ 25°C |
Typtone Bile X-Glucuronide (TBX) Medium (Dehydrated), Oxoid Preparation:
Suspend 36.6g of TBX Medium in 1 litre of distilled water. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C and pour the medium into sterile Petri dishes.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates of medium at 2-8°C.
Disk Dispenser 6 Place 90mm Skirt
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Thermo ScientificTM OxoidTM
Accurately place antimicrobial susceptibility disks onto your media of choice when carrying out manual Antimicrobial Susceptibility Testing methods using a self-tamping Thermo Scientific™ Oxoid™ Antimicrobial Susceptibility Disk Dispenser.
Midi Horizontal Electrophoresis Packages
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Cleaver Scientific
Cleaver Scientific now offers a comprehensive range of Combination Packages to suit any budget or horizontal electrophoresis application.
Co2gen Compact 20/Pck
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Thermo ScientificTM OxoidTM
Oxoid CO2 Gen Compact Sachet is for use in Compact Plastic Pouch.
- No water or catalyst required
- No hydrogen gas produced
- For use with Compact Plastic Pouch, Part No. AG0020C and AnaeroGen Compact Sealing Clip, Part No. AN0005C
Minimum Essential Medium Eagle (EMEM) with Alpha Modification, powder form
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Sigma-Aldrich
This is the most enriched variation of the EMEM formulation offered that suitable for cell culuture. It contains all 21 normal amino acids, some at increased concentrations. In addition, it contains 5 additional vitamins. Refer to the table below and choose your preferecd EMEM!
OEM SeaKem® LE Agarose
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Lonza
Product Overview
SeaKem® LE Agarose was the first and is still the world’s most trusted agarose for nucleic acid electrophoresis. Ideal for routine analysis of nucleic acids by gel electrophoresis and blotting, each SeaKem® LE Gel sharply resolves DNA and provides consistent resolution from lot-to-lot. This molecular biology grade agarose has no detectable DNase or RNase activity and forms strong gels with low background upon ethidium bromide, SYBR® Green, or GelStarTM Staining. Due to its low EEO, DNA will have a high electrophoretic mobility. SeaKem® LE Agarose is also recommended for protein gel electrophoresis in applications such as Ouchterlony and radial immunodiffusion (RID). Information about bulk and custom packaging is available through Lonza’s Customer Service Team.
Benefits
Applications
Content & Storage
1 kb DNA Ladder, NEB
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New England Biolabs
This 1 kb DNA ladder is available in Quick-Load Purple format – enjoy the convenience of a ready-to-load format.
1 kb DNA ladder from New England Biolabs (NEB) is suitable for use with molecular weight standard for agarose gel electrophoresis. A number of proprietary plasmids are digested to completion with appropriate restriction enzymes to yield 10 bands, ranging from 0.5 to 10 kilobases. NEB set 3.0 kb fragment as a reference band by increasing the intensity of that band.
- Size range: 500 bp to 10 kb
- Value pricing
- Supplied with a free vial of Gel Loading Dye, Purple (6X), no SDS (NEB #B7025).
- Easy to identify reference bands
- Small size suitable for 200 gel lanes; large size suitable for 1000 gel lanes
Q5 Hot Start High-Fidelity 2X Master Mix
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New England Biolabs
Fidelity at its finest
Q5® High-Fidelity DNA Polymerase sets a new standard for both fidelity and robust performance. With the highest fidelity amplification available (~280 times higher than Taq), Q5 DNA Polymerase results in ultra-low error rates. Q5 DNA Polymerase is composed of a novel polymerase that is fused to the processivity-enhancing Sso7d DNA binding domain, improving speed, fidelity and reliability of performance.
- Highest fidelity amplification (~280X higher than Taq)
- Ultra-low error rates
- Superior performance for a broad range of amplicons (from high AT to high GC)
- Hot start and master mix formats available
FEATURED PRODUCTS
PYR/Esculin Disk 25Disk/VL
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Thermo Scientific™ Remel™
- Thermo Scientific™ Remel PYR/Esculin Disc is used for presumptive identification of group A streptococci and enterococci.
- Requires PYR Reagent, Part No. R21544 or R21258.
F100 Nitrofurantoin
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Thermo ScientificTM OxoidTM
FastPrep-24™ 5G Grinder
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MP Biomedicals
Description
The FastPrep-24 5G is a versatile sample disruption instrument designed to quickly and efficiently homogenize, grind, or lyse biological samples. A completely self-contained system, the FastPrep-24 5G eliminates the risk of cross-contamination and time-consuming clean-up associated with manual lysis methods. Samples are added to individual lysing matrix tubes and placed into sample holder adapters within the instrument. The unique, optimized motion of the FastPrep-24 5G shakes the samples in a tridimensional motion resulting in the collision of the sample material with the lysing matrix particles.
Intuitive software makes programming the FastPrep-24 5G fast and simple. The programmable touchscreen display allows for the use of pre-determined or custom homogenization speeds and duration times with a finger touch. Increased power features result in faster processing times and complete lysis of even the toughest samples.
Key application include:
-Suitable for all sample types
-Lysis of yeast, fungi, and bacteria, soft tissues, cells
-Isolation of PCR-Ready Genomic DNA from fecal or soil samples
-Isolation of PCR-Ready Genomic DNA from fungi and bacteria samples
-DNA & RNA Purification
-Breaks hard/brittle samples
-Isolate DNA from virtually any source.
-Lysis of highly contagious samples
-Grinding of teeth, bones, hair and pulverization of building materials
Please contact us for pricing and availability.
Schaedler Broth 500g
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Thermo ScientificTM OxoidTM
- Oxoid Schaedler Anaerobe Broth is a thioglycollate-free broth for the general growth of anaerobes.
- For use in blood cultures and antibiotic MIC studies.
Schaedler Anaerobe Broth, Oxoid Composition
| Typical Formula* | gm/litre |
| Tryptone Soya Broth (Oxoid CM129) | 10.0 |
| Special peptone | 5.0 |
| Yeast extract | 5.0 |
| Glucose | 5.0 |
| Cysteine HCl | 0.4 |
| Haemin | 0.01 |
| Tris Buffer | 0.75 |
| pH 7.6 ± 0.2 @ 25°C |
Schaedler Anaerobe Broth, Oxoid Preparation:
Add 26.5g to 1 litre of distilled water and mix to dissolve completely. Dispense into final containers and sterilise by autoclaving at 121°C for 15 minutes.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store prepared broth in the dark at <15°C.
Precautions
As with all anaerobic broth media, it is important to avoid chemo-oxidation (overheating) and photo-oxidation (storage in the light) because such oxidative effects cause the inhibition of growth.
Anti-p62 (Sequestosome-1), clone 11C9.2 (mouse monoclonal)
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Merck
Description
p62 is also known as sequestosome-1, and is a 62 kDa ras-GAP associated protein. p62 is thought to bind to src-family tyrosine kinases and may also provide a linker action for activated src-family kinases with their downstream effectors. p62 has an RNA-binding region and may have RNA-binding ability. p62 is associated with activation of transcription factor NF-κB, involved in cell differentiation, immune response and potassium channel regulation. p62 has recently been linked to extrinsic apoptosis and may be a key player in tumorigenesis. Research focus area: Cancer.
Key Applications:
-Western Blotting
-Flow Cytometry
-Immunocytochemistry
Purified Agar 500g
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Thermo ScientificTM OxoidTM
Oxoid Purified Agar is an exceptionally bright and clear agar recommended for electrophoresis studies but also suitable for many bacteriological purposes.
Quick-Load 1 kb DNA Ladder
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New England Biolabs
This product is available in Quick-Load Purple format – enjoy sharper bands, no UV shadow, and a lower price.
- Size range: 500 bp to 10 kb
- Convenient, ready-to-load format
- Formulated with bromophenol blue dye
- Easy to identify reference bands
Eppendorf Xplorer® Multi-Channel Electronic Micropipettes
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Eppendorf
Description
The Eppendorf Xplorer multichannel electronic pipettes are designed to aspirate and dispense liquid from 0.5µl to 1250µl. The use of these electronic pipettes helps to reduce the human error risk, which greatly enhances the reproducibility and consistency of your research results. Moreover, the Xplorer multichannel digital pipettes are the ideal micropipette if you need precise and accurate results. At the same time, the operating concept and ergonomic design of Eppendorf pipette are supported by Eppendorf PhysioCare Concept®, which help you to experience fatigue-free pipetting and always maintaining full control over pipetting procedures.
Key Features:
- Everything under control – All information on the pipette display is clearly readable, regardless of your hand position or work position.
- Simple logic – Logical pipette operation with the unique multi-function rocker
- Ergonomics means to never feel the effects of hard work – The perfect balance and hand rest of the Eppendorf Xplorer electronic pipettes guarantee a low-impact position and efficient means for relaxation periods.
- Spring-loaded tip cone for reliable tip fit and low tip attachment forces – The spring limits the attachment forces and at the same time guarantees a reproducible and safe tip fit. It provides increased user to user reproducibility for more uniform results among members of the lab.
- SOFTeject reduces tip ejection forces by 50% – During a single tip ejection stroke, the outside tips eject before the inside tips. You just slightly press the ejection button once, and automatically the tips are ejected successively.
TWEEN® 20 BioXtra (Sigma-Aldrich)
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Sigma-Aldrich
Description
CAS number: 9005-64-5
Suitability: Suitable for blocking, cell culture, hybridization, protein purification, transformation
Synonym: Polyethylene glycol sorbitan monolaurate, Polyoxyethylenesorbitan monolaurate
General description
Tween®20 is a polyoxyethylene sorbitol ester that belongs to the polysorbate family. It is a nonionic detergent having a molecular weight of 1,225 daltons, assuming 20 ethylene oxide units, 1 sorbitol, and 1 lauric acid as the primary fatty acid. The ethylene oxide subunits are responsible for the hydrophilic nature of the surfactant, while the hydrocarbon chains provide the hydrophobic environment. Sorbitol forms the backbone ring to which the ethylene oxide polymers are attached.
Application
TWEEN 20 is a non-ionic detergent that has been used in a variety of applications, including:
- Post-hybridization wash buffer after in situ hybridization.
- Used along with PBS (phosphate-buffered saline) as the washing buffer for ELISA and immunostaining.
- Acted as an emulsifying agent for the preparation of stable oil-in-water emulsions
- Used in pre-extraction of membranes to remove peripheral proteins (used at 2% for extraction of membrane-bound proteins).
- Acted as a blocking agent for membrane-based immunoassays at a typical concentration of 0.05%.
- Used for lysing mammalian cells at a concentration of 0.005 to 0.5%.
CR-SMAC 500g
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Thermo ScientificTM OxoidTM
- Oxoid Cefixime Rhamnose Sorbitol MacConkey Agar Base (CR-SMAC) is a selective and differential medium containing rhamnose and cefixime for the isolation of E. coli O157.
- Also available Cefixime Supplement, Part No. SR0191E.
CR-SMAC Agar Base, Oxoid Composition
| Typical Formula* | gm/litre |
| Peptone | 20.0 |
| Sorbitol | 10.0 |
| Bile Salts No. 3 | 1.5 |
| Sodium chloride | 5.0 |
| Rhamnose | 5.0 |
| Neutral red | 0.03 |
| Crystal violet | 0.001 |
| Agar | 15.0 |
| pH 7.1 ± 0.2 @ 25°C |
CR-SMAC Agar, Oxoid Preparation:
Suspend 28.25g in 500ml of distilled water. Mix well and sterilise by autoclaving at 121°C for 15 minutes. Cool to approximately 50°C and aseptically add the contents of one vial of Cefixime Supplement SR0191, reconstituted as directed. Mix well and pour into sterile Petri dishes.
To reconstitute Cefixime Supplement, aseptically add 2ml sterile distilled water to 1 vial of supplement. Mix gently to dissolve.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium for up to 2 weeks at 2-8°C.
TWEEN® 20 for Molecular Biology (Sigma-Aldrich)
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Sigma-Aldrich
Description
CAS number: 9005-64-5
Suitability: Suitable for molecular biology
Synonym: Polyethylene glycol sorbitan monolaurate, Polyoxyethylenesorbitan monolaurate
General description
Tween®20 is a polyoxyethylene sorbitol ester that belongs to the polysorbate family. It is a nonionic detergent having a molecular weight of 1,225 daltons, assuming 20 ethylene oxide units, 1 sorbitol, and 1 lauric acid as the primary fatty acid. The ethylene oxide subunits are responsible for the hydrophilic nature of the surfactant, while the hydrocarbon chains provide the hydrophobic environment. Sorbitol forms the backbone ring to which the ethylene oxide polymers are attached.
Application
TWEEN 20 is a non-ionic detergent that widely used in biochemical applications. It has been used:
• As an emulsifying agent for the preparation of stable oil-in-water emulsions
• In pre-extraction of membranes to remove peripheral proteins (used at 2% for extraction of membrane-bound proteins)
• As a blocking agent for membrane-based immunoassays at a typical concentration of 0.05%
• For lysing mammalian cells at a concentration of 0.005 to 0.5%
• Along with PBS in the dilution of antibodies in immunohistochemistry technique
• For washing cells in FISH (Fluorescence in situ hybridization) technique
Basic Fuchsin – Technical Dye (10G)
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Thermo ScientificTM OxoidTM
Oxoid Basic Fuchsin Indicator is a dye for use with Endo Agar Base, Part No. CM0479B and m Endo (LES) Agar Base, Part No. MM0551B.
Basic Fuchsin Preparation:
BR0050 should be dissolved to create a 10% w/v solution using 95% ethyl alcohol.
For each litre of culture medium use the amount described in the product reconstitution details. This should be in a 10% w/v solution of the dye dissolved in 50:50 ethanol/distilled water. E.g. For CM0479 add 6ml of a 10% w/v Basic Fuchsin solution per litre of medium.
The magenta dyes are closely related to known carcinogenic substances; therefore basic fuchsin should be handled with care, avoiding inhaling the powder or staining the skin with dye.
Minimum Essential Medium Eagle (EMEM) with Alpha Modification, powder form
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Sigma-Aldrich
This is the most enriched variation of the EMEM formulation offered that suitable for cell culuture. It contains all 21 normal amino acids, some at increased concentrations. In addition, it contains 5 additional vitamins. Refer to the table below and choose your preferecd EMEM!
OEM SeaKem® LE Agarose
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Lonza
Product Overview
SeaKem® LE Agarose was the first and is still the world’s most trusted agarose for nucleic acid electrophoresis. Ideal for routine analysis of nucleic acids by gel electrophoresis and blotting, each SeaKem® LE Gel sharply resolves DNA and provides consistent resolution from lot-to-lot. This molecular biology grade agarose has no detectable DNase or RNase activity and forms strong gels with low background upon ethidium bromide, SYBR® Green, or GelStarTM Staining. Due to its low EEO, DNA will have a high electrophoretic mobility. SeaKem® LE Agarose is also recommended for protein gel electrophoresis in applications such as Ouchterlony and radial immunodiffusion (RID). Information about bulk and custom packaging is available through Lonza’s Customer Service Team.
Benefits
Applications
Content & Storage
1 kb DNA Ladder, NEB
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New England Biolabs
This 1 kb DNA ladder is available in Quick-Load Purple format – enjoy the convenience of a ready-to-load format.
1 kb DNA ladder from New England Biolabs (NEB) is suitable for use with molecular weight standard for agarose gel electrophoresis. A number of proprietary plasmids are digested to completion with appropriate restriction enzymes to yield 10 bands, ranging from 0.5 to 10 kilobases. NEB set 3.0 kb fragment as a reference band by increasing the intensity of that band.
- Size range: 500 bp to 10 kb
- Value pricing
- Supplied with a free vial of Gel Loading Dye, Purple (6X), no SDS (NEB #B7025).
- Easy to identify reference bands
- Small size suitable for 200 gel lanes; large size suitable for 1000 gel lanes
Q5 Hot Start High-Fidelity 2X Master Mix
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New England Biolabs
Fidelity at its finest
Q5® High-Fidelity DNA Polymerase sets a new standard for both fidelity and robust performance. With the highest fidelity amplification available (~280 times higher than Taq), Q5 DNA Polymerase results in ultra-low error rates. Q5 DNA Polymerase is composed of a novel polymerase that is fused to the processivity-enhancing Sso7d DNA binding domain, improving speed, fidelity and reliability of performance.
- Highest fidelity amplification (~280X higher than Taq)
- Ultra-low error rates
- Superior performance for a broad range of amplicons (from high AT to high GC)
- Hot start and master mix formats available
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