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TWEEN® 20 (Sigma-Aldrich)

RM179.00RM210.00
Brand:

Sigma-Aldrich

Description

CAS number: 9005-64-5
Suitability: Suitable for DNA extraction, RNA extraction, cell culture and protein purification
Synonym: Polyethylene glycol sorbitan monolaurate, Polyoxyethylenesorbitan monolaurate

General description

Tween®20 is a polyoxyethylene sorbitol ester that belongs to the polysorbate family. It is a nonionic detergent having a molecular weight of 1,225 daltons, assuming 20 ethylene oxide units, 1 sorbitol, and 1 lauric acid as the primary fatty acid. The ethylene oxide subunits are responsible for the hydrophilic nature of the surfactant, while the hydrocarbon chains provide the hydrophobic environment. Sorbitol forms the backbone ring to which the ethylene oxide polymers are attached.

Application

TWEEN 20 has been used as a component of:
• washing buffer and blocking buffer in Western blotting
• blocking buffer in immunohistochemistry
• washing buffer in ELISA (enzyme-linked immunosorbent assay)
• reaction mixture in PCR (polymerase chain reaction)
• emulsifying agent for the preparation of stable oil-in-water emulsions.
• pre-extraction of membranes to remove peripheral proteins (used at 2% for extraction of membrane-bound proteins)
• blocking agent for membrane-based immunoassays at a typical concentration of 0.05%
• lysing mammalian cells at a concentration of 0.005 to 0.5%

C30 Chloramphenicol

RM60.00
Brand:

Thermo ScientificTM OxoidTM

Manually determine the antibiotic susceptibility of microorganisms using Antimicrobial Susceptibility Testing (AST) methods in conjunction with reliable, easy-to-use Thermo Scientific™ Oxoid™ Chloramphenicol Antimicrobial Susceptibility Disks.

EagI

RM395.00RM1,430.00
Brand:

New England Biolabs

Having supplied restriction enzymes to the research community for over 40 years, NEB has earned the reputation of being the leader in enzyme technologies.  Working continuously to be worth of that distinction, NEB strives to develop enzyme of the highest purity and unparalleled quality.

BsrGI

RM410.00RM1,495.00
Brand:

New England Biolabs

Having supplied restriction enzymes to the research community for over 40 years, NEB has earned the reputation of being the leader in enzyme technologies. Working continuously to be worthy of that distinction, NEB strives to develop enzymes of the highest purity and unparalleled quality.

FastDNA 50ml Spin Kit for Soil

RM1,114.00
Brand:

MP Biomedicals

Up to 5 g of soil are processed by the FastPrep®-24 or FastPrep®-96 instrument with the Lysing Matrix E tubes designed to efficiently lyse all microorganisms including difficult sources such as eubacterial spores and endospores, gram positive bacteria and yeast. The released DNA is purified by a silica-based spin filter columns and removes humic acids/polyphenols that can inhibit PCR. The resulting pure DNA is suitable for PCR analysis and other downstream applications. Research use only. Key applications include isolation of bacterial, fungi, plant and genomic DNA from soil and evnironmental samples.

Gentamycin 1024 M.I.C.E. (50 Pack)

RM0.00
Brand:

Thermo ScientificTM OxoidTM

**This product is not available at the moment.

Effortlessly establish accurate Minimum Inhibitory Concentration (MIC) values manually using Thermo Scientific™ Oxoid™ Gentamycin M.I.C.Evaluator Strips (M.I.C.E.™).

Sorbitol MacConkey Agar 500g

RM437.00
Brand:

Thermo ScientificTM OxoidTM

  • Oxoid Sorbitol MacConkey Agar is a selective differential medium for the detection of E. coli O157.
  • Also available C-T Supplement, Part No. SR0172E and SR0172H.

Clindamycin 256 M.I.C.E. (10 pack)

RM0.00
Brand:

Thermo ScientificTM OxoidTM

**This product is not available at the moment.

Effortlessly establish accurate Minimum Inhibitory Concentration (MIC) values manually using Thermo Scientific™ Oxoid™ Clindamycin M.I.C.Evaluator Strips (M.I.C.E.™).

Rappaport Vassiliadis Soy Peptone 500g

RM282.00
Brand:

Thermo ScientificTM OxoidTM

Selectively enrich Salmonella spp. from food and environmental specimens with Thermo Scientific™ Oxoid™ Rappaport-Vassiliadis Soya Peptone Broth (Dehydrated). Rappaport- Vassiliadis Soya Peptone Broth selects for Salmonella species by exploiting their characteristics when compared with other Enterobacteriaceae, such as the ability to survive at relatively high osmotic pressure, multiply at relatively low pH values, be relatively more resistant to malachite green, and have relatively less demanding nutritional requirements.

MRS Broth 500g

RM253.00
Brand:

Thermo ScientificTM OxoidTM

Isolate and enumerate lactic acid bacteria from food with Thermo Scientific™ Oxoid™ MRS Broth (DeMan, Rogosa and Sharpe) (Dehydrated). This non-selective medium is designed to support profuse growth of lactic acid bacteria.

Chloramphenicol Supplement 500ml

RM230.00
Brand:

Thermo ScientificTM OxoidTM

For the isolation of yeast and molds associated with spoilage, add Oxoid Chloramphenicol Selective Supplement to DRBC Agar Base, Part No. CM0727B; for the enumeration of yeast and molds in foods, add to Rose Bengal Chloramphenicol Agar Base, Part No. CM0549B.

Water Standard 1%, CRM for Karl Fischer Titration (Merck)

RM367.00
Brand:

Merck

CAS no.: 1589-47-5, 107-98-2
Chemical Formula: –
Molecular Weight: –
Synonym: Water standard 1% Certified Reference Material for Karl Fischer Titration 1 g ≙ 10 mg H₂O Aquastar®
Quality level: MQ300
Product specification for Water standard 1% CRM for Karl Fischer Titration

OmniPur Water, WFI Quality, Sterile Purified Water, Cell Culture Tested – CAS7732-18-5

RM100.00 RM69.99
Brand:

Merck

OmniPur Water is sterile water that is tested to be DNase free, Protease free, RNase free and bacterial free.

It is suitable to be used in:

  • Cell Biology
    • Cell culture media preparation, extraction buffer, Molecular work, reconstitute of compound
    • Diluent for live cells staining
  • Sterile solution preparation Microbiology Sample Preparation
    • Diluent and rinsing reagent in Microbial Membrane Filtration

Key Specification:

  1. Sterile by USP Standard
  2. DNase, RNase, Protease-Free
  3. < 500 ppb Total Organic Carbon
  4. < 0.025 EU/ml Bacterial Endotoxin

COA for Omnipur Sterile Water

Minimum Essential Medium Eagle (EMEM) with Alpha Modification, powder form

RM347.00RM553.00
Brand:

Sigma-Aldrich

This is the most enriched variation of the EMEM formulation offered that suitable for cell culuture. It contains all 21 normal amino acids, some at increased concentrations. In addition, it contains 5 additional vitamins. Refer to the table below and choose your preferecd EMEM!

Minimum Essential Medium Eagle (EMEM) with Alpha Modification, powder form Brochure

OEM SeaKem® LE Agarose

RM1,250.00 RM999.00
Brand:

Lonza

Product Overview
SeaKem® LE Agarose was the first and is still the world’s most trusted agarose for nucleic acid electrophoresis. Ideal for routine analysis of nucleic acids by gel electrophoresis and blotting, each SeaKem® LE Gel sharply resolves DNA and provides consistent resolution from lot-to-lot. This molecular biology grade agarose has no detectable DNase or RNase activity and forms strong gels with low background upon ethidium bromide, SYBR® Green, or GelStarTM Staining. Due to its low EEO, DNA will have a high electrophoretic mobility. SeaKem® LE Agarose is also recommended for protein gel electrophoresis in applications such as Ouchterlony and radial immunodiffusion (RID). Information about bulk and custom packaging is available through Lonza’s Customer Service Team.

Benefits

  • All Lonza Agarose is quality tested to certify performance
  • High gel strength
  • Low EEO
  • Standard melting temperature

Applications

  • Standard DNA/RNA Electrophoreisis

Content & Storage

  • SeaKem® LE Agarose 500 g

BamHI-HF, NEB

RM370.00RM1,330.00
Brand:

New England Biolabs

BamHI-HF, NEB has the same specificity as BamHI, but have been engineered for significantly reduced star activity and performance in a single buffer (CutSmart® Buffer).  All HF-restriction enzymes come with Gel Loading Dye, Purple (6X).  Enjoy the enhanced performance and added value of our engineered enzymes at the same price as the native enzyme:

  • Engineered for improved performance.
  • 100% activity in CutSmart Buffer.
  • Time-Saver™ qualified for digestion in 5-15 minutes.
  • Reduced star activity.
  • Supplied with 1 vial of Gel Loading Dye, Purple (6X).

1 kb DNA Ladder, NEB

RM335.00RM1,140.00
Brand:

New England Biolabs

This 1 kb DNA ladder is available in Quick-Load Purple format – enjoy the convenience of a ready-to-load format.

1 kb DNA ladder from New England Biolabs (NEB) is suitable for use with molecular weight standard for agarose gel electrophoresis. A number of proprietary plasmids are digested to completion with appropriate restriction enzymes to yield 10 bands, ranging from 0.5 to 10 kilobases. NEB set 3.0 kb fragment as a reference band by increasing the intensity of that band.

  • Size range: 500 bp to 10 kb
  • Value pricing
  • Supplied with a free vial of Gel Loading Dye, Purple (6X), no SDS (NEB #B7025).
  • Easy to identify reference bands
  • Small size suitable for 200 gel lanes; large size suitable for 1000 gel lanes

Q5 Hot Start High-Fidelity 2X Master Mix

RM1,145.00RM4,370.00
Brand:

New England Biolabs

Fidelity at its finest

Q5® High-Fidelity DNA Polymerase sets a new standard for both fidelity and robust performance. With the highest fidelity amplification available (~280 times higher than Taq), Q5 DNA Polymerase results in ultra-low error rates. Q5 DNA Polymerase is composed of a novel polymerase that is fused to the processivity-enhancing Sso7d DNA binding domain, improving speed, fidelity and reliability of performance.

  • Highest fidelity amplification (~280X higher than Taq)
  • Ultra-low error rates
  • Superior performance for a broad range of amplicons (from high AT to high GC)
  • Hot start and master mix formats available

FEATURED PRODUCTS

Visit our shop to see amazing products.

100 bp DNA Ladder, NEB

RM355.00RM1,235.00
Brand:

New England Biolabs

 This product is available in Quick-Load Purple format – enjoy the convenience of a ready-to-load format.

  • Size range: 100  bp – 1,517 bp
  • Supplied with free vial of Gel Loading Dye, Purple (6X), no SDS (NEB #B7025).
  • Easy-to-identify reference bands
  • Value pricing
  • Small size suitable for 100 gel lanes; large size suitable for 500 gel lanes

BactiDrop VP-B (40% KOH) PK/50

RM400.00
Brand:

Thermo Scientific™ Remel™

Remel BactiDrop Voges-Proskauer B is used for detection of acetylmethylcarbinol from glucose fermentation.

  • Used in conjunction with VP-A, Part No. R21560
  • For use with MRVP Broth

Ordering Alerts:

BactiDrop reagents are supplied in crushable ampules encased in a protective plastic sheath with a convenient dropper dispenser tip.

FokI

RM410.00RM1,495.00
Brand:

New England Biolabs

Having supplied restriction enzymes to the research community for over 40 years, NEB has earned the reputation of being the leader in enzyme technologies.  Working continuously to be worth of that distinction, NEB strives to develop enzyme of the highest purity and unparalleled quality.

  • 100% activity in CutSmart® Buffer (over 210 enzymes are available in the same buffer) allowing for easier double digests
  • Type IIS restriction enzymes recognize asymmetric DNA sequences and cleave outside of their recognition sequence
  • Requires two or more sites for cleavage. Please review the enzyme list and recommendations in the following table as well as the background information article.

E5 Erythromycin

RM0.00
Brand:

Thermo ScientificTM OxoidTM

** This item is not available at the moment. Please contact us for more information.
Manually determine the antibiotic susceptibility of microorganisms using Antimicrobial Susceptibility Testing (AST) methods in conjunction with reliable, easy-to-use Thermo Scientific™ Oxoid™ Erythromycin Antimicrobial Susceptibility Disks.

C10 Chloramphenicol

RM0.00
Brand:

Thermo ScientificTM OxoidTM

** This item is not available at the moment. Please contact us for more information.
Manually determine the antibiotic susceptibility of microorganisms using Antimicrobial Susceptibility Testing (AST) methods in conjunction with reliable, easy-to-use Thermo Scientific™ Oxoid™ Chloramphenicol Antimicrobial Susceptibility Disks.

Crossley Milk Medium 500g

RM312.00
Brand:

Thermo ScientificTM OxoidTM

A simple medium originally described by Crossley for the routine examination of canned food samples for anaerobic bacteria, this medium has evolved as the result of comparative trials carried out by Crossley with several standard media. It is capable of giving rapid growth without the use of special anaerobic apparatus, yet the bacteria detected may be provisionally identified by their reactions upon the medium.

TB Decolorizer (3% acid alc) BTLx5/250ml

RM459.00
Brand:

Thermo Scientific™ Remel™

Thermo Scientific™ Remel TB Decolorizer is for TB Kinyoun and Ziehl-Neelsen stains.

Cec30 Cefaclor

RM60.00
Brand:

Thermo ScientificTM OxoidTM

Manually determine the antibiotic susceptibility of microorganisms using Antimicrobial Susceptibility Testing (AST) methods in conjunction with reliable, easy-to-use Thermo Scientific™ Oxoid™ Cefaclor Antimicrobial Susceptibility Disks.

Legionella CYE Agar Base 500g

RM305.00
Brand:

Thermo ScientificTM OxoidTM

Isolate Legionella from environmental samples and clinical specimens using Thermo Scientific™ Oxoid™ Legionella CYE Agar Base when used with Legionella Growth Supplement SR01110. Legionella CYE Agar Base is a non-selective medium that exhibits optimal recovery of legionellaceae in short incubation periods from both environmental samples and clinical specimens.

TE Buffer Solution pH 8.0 (Merck)

RM210.00
Brand:

Merck

Description

1X TE Solution, pH 8.0 (10mM TRIS-HCl, 1mM EDTA). Molecular Biology grade.

Specifications:
Conductivity at 25°C: 775-975 µmho
DNase: None detected
pH of solution at 25°C: 7.90-8.10
Protease: None detected
RNase: None detected

MSDS for TE Buffer pH8.0 (Merck).

Maximum Recovery Diluent 500g

RM171.00
Brand:

Thermo ScientificTM OxoidTM

Achieve maximum recovery of microorganisms during dilution with this protective, isotonic Thermo Scientific™ Oxoid™ Maximum Recovery Diluent (Dehydrated).

Maximum Recovery Diluent combines the protective effect of peptone in the diluting solution1 with the osmotic support of physiological saline. The low peptone concentration in the diluent reduces organism multiplication in the sample for at least 45 minutes during the dilution stage.

NsiI

RM395.00RM1,430.00
Brand:

New England Biolabs

Having supplied restriction enzymes to the research community for over 40 years, NEB has earned the reputation of being the leader in enzyme technologies.  Working continuously to be worth of that distinction, NEB strives to develop enzyme of the highest purity and unparalleled quality.

OmniPur Water, WFI Quality, Sterile Purified Water, Cell Culture Tested – CAS7732-18-5

RM100.00 RM69.99
Brand:

Merck

OmniPur Water is sterile water that is tested to be DNase free, Protease free, RNase free and bacterial free.

It is suitable to be used in:

  • Cell Biology
    • Cell culture media preparation, extraction buffer, Molecular work, reconstitute of compound
    • Diluent for live cells staining
  • Sterile solution preparation Microbiology Sample Preparation
    • Diluent and rinsing reagent in Microbial Membrane Filtration

Key Specification:

  1. Sterile by USP Standard
  2. DNase, RNase, Protease-Free
  3. < 500 ppb Total Organic Carbon
  4. < 0.025 EU/ml Bacterial Endotoxin

COA for Omnipur Sterile Water

Minimum Essential Medium Eagle (EMEM) with Alpha Modification, powder form

RM347.00RM553.00
Brand:

Sigma-Aldrich

This is the most enriched variation of the EMEM formulation offered that suitable for cell culuture. It contains all 21 normal amino acids, some at increased concentrations. In addition, it contains 5 additional vitamins. Refer to the table below and choose your preferecd EMEM!

Minimum Essential Medium Eagle (EMEM) with Alpha Modification, powder form Brochure

OEM SeaKem® LE Agarose

RM1,250.00 RM999.00
Brand:

Lonza

Product Overview
SeaKem® LE Agarose was the first and is still the world’s most trusted agarose for nucleic acid electrophoresis. Ideal for routine analysis of nucleic acids by gel electrophoresis and blotting, each SeaKem® LE Gel sharply resolves DNA and provides consistent resolution from lot-to-lot. This molecular biology grade agarose has no detectable DNase or RNase activity and forms strong gels with low background upon ethidium bromide, SYBR® Green, or GelStarTM Staining. Due to its low EEO, DNA will have a high electrophoretic mobility. SeaKem® LE Agarose is also recommended for protein gel electrophoresis in applications such as Ouchterlony and radial immunodiffusion (RID). Information about bulk and custom packaging is available through Lonza’s Customer Service Team.

Benefits

  • All Lonza Agarose is quality tested to certify performance
  • High gel strength
  • Low EEO
  • Standard melting temperature

Applications

  • Standard DNA/RNA Electrophoreisis

Content & Storage

  • SeaKem® LE Agarose 500 g

1 kb DNA Ladder, NEB

RM335.00RM1,140.00
Brand:

New England Biolabs

This 1 kb DNA ladder is available in Quick-Load Purple format – enjoy the convenience of a ready-to-load format.

1 kb DNA ladder from New England Biolabs (NEB) is suitable for use with molecular weight standard for agarose gel electrophoresis. A number of proprietary plasmids are digested to completion with appropriate restriction enzymes to yield 10 bands, ranging from 0.5 to 10 kilobases. NEB set 3.0 kb fragment as a reference band by increasing the intensity of that band.

  • Size range: 500 bp to 10 kb
  • Value pricing
  • Supplied with a free vial of Gel Loading Dye, Purple (6X), no SDS (NEB #B7025).
  • Easy to identify reference bands
  • Small size suitable for 200 gel lanes; large size suitable for 1000 gel lanes

Q5 Hot Start High-Fidelity 2X Master Mix

RM1,145.00RM4,370.00
Brand:

New England Biolabs

Fidelity at its finest

Q5® High-Fidelity DNA Polymerase sets a new standard for both fidelity and robust performance. With the highest fidelity amplification available (~280 times higher than Taq), Q5 DNA Polymerase results in ultra-low error rates. Q5 DNA Polymerase is composed of a novel polymerase that is fused to the processivity-enhancing Sso7d DNA binding domain, improving speed, fidelity and reliability of performance.

  • Highest fidelity amplification (~280X higher than Taq)
  • Ultra-low error rates
  • Superior performance for a broad range of amplicons (from high AT to high GC)
  • Hot start and master mix formats available

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