1 kb DNA Ladder, NEB
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New England Biolabs
This 1 kb DNA ladder is available in Quick-Load Purple format – enjoy the convenience of a ready-to-load format.
1 kb DNA ladder from New England Biolabs (NEB) is suitable for use with molecular weight standard for agarose gel electrophoresis. A number of proprietary plasmids are digested to completion with appropriate restriction enzymes to yield 10 bands, ranging from 0.5 to 10 kilobases. NEB set 3.0 kb fragment as a reference band by increasing the intensity of that band.
- Size range: 500 bp to 10 kb
- Value pricing
- Supplied with a free vial of Gel Loading Dye, Purple (6X), no SDS (NEB #B7025).
- Easy to identify reference bands
- Small size suitable for 200 gel lanes; large size suitable for 1000 gel lanes
1 kb Plus DNA Ladder, NEB
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New England Biolabs
This 1kb plus DNA ladder, NEB was previously named the 2-Log DNA Ladder. There have been no changes to product formulation or specifications. This product is available in Quick-Load Purple format – enjoy the convenience of a ready-to-load format.
A single convenient ladder for 100 bp up to 10 kb (100 bp and 1 kb ladder in one!)
- Value pricing
- Supplied with a free vial of Gel Loading Dye, Purple (6X), no SDS (NEB #B7025)
- Easy to identify reference bands
- Small size suitable for 200 gel lanes; large size suitable for 1000 gel lanes
100 bp DNA Ladder, NEB
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New England Biolabs
This product is available in Quick-Load Purple format – enjoy the convenience of a ready-to-load format.
- Size range: 100 bp – 1,517 bp
- Supplied with free vial of Gel Loading Dye, Purple (6X), no SDS (NEB #B7025).
- Easy-to-identify reference bands
- Value pricing
- Small size suitable for 100 gel lanes; large size suitable for 500 gel lanes
1X and 10X TBE buffer (Tris-Borate-EDTA), Biobasic
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Bio Basic
Description
This product is currently not available. Please contact us for more information.
TBE buffer (Tris-Borate-EDTA, Biobasic) is commonly used in all DNA electrophoresis applications (both for acrylamide and for agarose gels). In general, TBE buffer offers better resolution of 0.1 to 3 kb fragments; whereas, TAE (Tris-Acetate-EDTA) buffer provides better resolution of fragments greater than 4 kb. Furthermore, TBE is better suited for high-voltage (>150V) electrophoresis because of its higher buffering capacity and lower conductivity than TAE. BIO BASIC INC. offers TBE in a number of different formats. 10X TBE liquid concentrate can be used to easily prepare a 1X TBE working solution by diluting with distilled, deionized water. 10X TBE Powder allows for the stable storage of the pre-mixed powder form that can be dissolved in distilled, deionized water to yield 1 liter each of 10X TBE buffer solution.
How to prepare a 10X TBE Buffer?
10X TBE buffer preparation is easy. Simply mix 10X TBE powder (SKU: A00269-500ml/A0026-4L) with an appropriate volume of distilled/deionized water. The 10X TBE buffer is ready to use now.
50 bp DNA Ladder, NEB
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New England Biolabs
This product is available in our Quick-Load Purple format – enjoy the convenience of a ready-to-load format.
- Size range: 50 bp – 1350 bp
- Supplied with a free vial of Gel Loading Dye, Purple (6X), no SDS (NEB #B7025)
- Easy-to-identify reference bands
- Small size suitable for 200 gel lanes; large size suitable for 1000 gel lanes
7-deaza dGTP
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New England Biolabs
- 7-deaza-dGTP (7-deaza-2′-deoxyguanosine 5′-triphosphate) is supplied as a 5 mM solution in Milli-Q® water as a lithium salt
- The nucleotide concentration is determined by measurements of absorbance at 257 nm
abTES COVID-19 qPCR I Kit (Novel Corona Virus)
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AITbiotech
Features:
- A qualitative real-time polymerase chain reaction (RT-PCR) kit which enables simultaneous detection of two COVID-19 specific regions from its non-structural polypeptide in a single reaction.
- Contains all the necessary PCR reagents for rapid, sensitive and specific detection using target-specific primers and double-labelled hydrolysis probes.
- Enables detection of the human housekeeping gene, GAPDH, as an Internal Control (IC) to identify possible PCR inhibitors from sample processing.
Product Performance:
- Validated Sample Types (as recommended by CDC)
- Nasopharyngeal wash/ aspirate or nasal aspirate
- Nasopharyngeal & oropharyngeal swabs
- Bronchoalveolar lavage
- Tracheal aspirate
- Sputum
- Serum
- Compatible Platforms
- AITbiotech anCyclerQ
- Bio-Rad CFX96
- Applied Biosystems 7500 Fast
- Regulatory Status
- CE-IVD
- Fluorescence
- Non-structural polypeptide NS1 (FAM)
- Non-structural polypeptide NS2 (Cy5)
- GAPDH (HEX)
** You may also interest in RNA Purification from Buccal Swabs, Nasopharyngeal Samples (swab or aspirate) and Saliva using the Monarch Total RNA Miniprep Kit.
Blue Prestained Protein Standard, Broad Range (11-250kDa)
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New England Biolabs
This product is a direct replacement of NEB# P7706 (Blue Prestained Protein Standard, Broad Range 11-190 kDa).
The Blue Prestained Protein Standard, Broad Range is a mixture of highly pure, recombinant, prestained proteins, covalently coupled with a blue chromophore, that resolves into 11 sharp bands when electrophoresed.
– Allows approximate molecular weight determination when performing SDS-PAGE analysis.
– Applications include verification of Western transfer efficiency on membranes and fluorescent imaging of SDS-PAGE.
– Direct loading, additional loading buffer and heat incubation not required
– Recombinant proteins with no detectable protease contaminating activities
– Optimal stability for up to 24 months
Blunt/TA Ligase Master Mix
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New England Biolabs
Blunt/TA Ligase Master Mix is a ready-to-use solution of T4 DNA Ligase, proprietary ligation enhancer, and optimized reaction buffer. This master mix is specifically formulated to improve ligation and transformation of both blunt-end and single-base overhang substrates.
- Simplifies reaction set-up, ensures an optimized ratio of enzyme and buffer components
- No thawing is necessary as it remains liquid during storage at -20°C
- Ligations for subcloning can be carried out in small volumes with low DNA concentrations, allowing users to conserve precious DNA samples and directly transform many strains of chemically competent E. coli without dilution
- Not sure which ligase to choose? Refer to our DNA and RNA Ligase Properties Chart
Breast Cancer Genetics – Electrophoresis Education kit
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Cleaver Scientific
Breastcancer is the most common type of cancer. About 5-10% of all breast cancers areinherited. Mutations in the breast cancer 1 (BRCA1) gene greatly increase therisk of developing breast and ovarian cancer. This gene is found on chromosome17 and is normally responsible for DNA repair. It is therefore called a tumoursuppressor gene. Mutations in BRCA1 are dominant and so the presence of onecopy of a mutated form can cause an increased risk of cancer.
This experiment will test DNA of members of a family where the grandmother (generation 2) and her daughter(generation 3) both died of breast cancer. The deceased women had a mutation intheir BRCA1 gene.
Each kit included enough DNA, agarose gel, electrophoesis buffer and DNA stain for up to 8 groups of students (8 gels).
Key Features
- Whole experiment takes less that 1 hour.
- Great for introducing genetic diseases and genetic screening to students
- Works perfectly with Cleaver Scientific electrophoresis equipment
Bst 2.0 DNA Polymerase
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New England Biolabs
Bst 2.0 DNA Polymerase displays improved amplification speed, yield and salt tolerance
- Optimized for Loop-Mediated Isothermal DNA Amplification (LAMP)
- Improved amplification reaction properties compared to Bst DNA Polymerase
- Fast polymerization
- Flexible reaction conditions including a higher salt tolerance and thermostability, as compared to wild-type Bst DNA Polymerase, Large Fragment (NEB #M0275)
Bst 2.0 WarmStart DNA Polymerase
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New England Biolabs
Optimized for Loop-Mediated Isothermal Amplification (LAMP)
- Consistent amplification performance with the convenience of room temperature setup
- WarmStart® technology eliminates off-target amplifilcation and offers increased reaction efficiency
- Optimal reaction performance from 60-72 °C
Bst 3.0 DNA Polymerase
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New England Biolabs
Bst 3.0 DNA Polymerase demonstrates robust performance even in high concentrations of amplification inhibitors and features significantly increased reverse transcriptase activity compared to Bst DNA Polymerase.
Bst DNA Polymerase Lg Frag
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New England Biolabs
The original polymerase for Loop-Mediated Isothermal Amplification (LAMP)
- Modified to retain 5´ → 3´ polymerase activity while lacking 5´ →3´ exonuclease activity
- Suitable for applications requiring thermophilic strand displacement
- Supplied with ThermoPol® Reaction Buffer for high product yields in demanding conditions
Cas9 Nuclease, S. pyogenes
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New England Biolabs
- Ideal for in vitro digestion of dsDNA
- Compatible with EnGen® sgRNA Synthesis Kit, S. pyogenes (NEB #E3322S) and the EnGen Mutation Detection Kit (NEB #E3321S)
- For help with oligo design, try EnGen sgRNA Template Oligo Designer – now included in the NEBioCalculator® Tool
Color Prestained Protein Standard, Broad Range (10-250 kDa)
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New England Biolabs
The Color Prestained Protein Standard, Broad Range is a mixture of highly pure, recombinant, prestained proteins, covalently coupled with a blue chromophore, and two reference bands (one orange and one green at 72 kDa and 26 kDa, respectively), that resolves into 11 sharp bands when electrophoresed.
- Allows approximate molecular weight determination when performing SDS-PAGE analysis
- Applications include verification of Western transfer efficiency on membranes and fluorescent imaging of SDS-PAGE
- Direct loading, additional loading buffer and heat incubation not required
- Recombinant proteins with no detectable protease contaminating activities
- Optimal stability for up to 24 months
Cystic Fibrosis – Electrophoresis Education kit
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Cleaver Scientific
Cystic Fibrosis is one of the most common genetic conditions in the UK. In this educational kit, students will learn about the basis of the disease, and run an agarose gel to seperate DNA samples from healthy patients, carriers of the disease and CF patients.
Each kit included enough DNA, agarose gel, electrophoesis buffer and DNA stain for up to 8 groups of students (8 gels).
Key Features
- Whole experiment takes less that 1 hour
- Great for introducing genetic diseases and genetic screening to students
- Works perfectly with Cleaver Scientific electrophoresis equipment
dATP Solution – 25 umol
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New England Biolabs
Enhance your PCR Reaction with our ultrapure dATP solution
- Compatible with all NEB DNA polymerases in a variety of applications
- ≥ 99% purity as determined by HPLC analysis
- Also available in solution mix (NEB #N0447) or set (NEB #N0446) format
Deionized Water
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MP Biomedicals
WATER, DNase, RNase-Free, is designed for those critical applications where DNase and RNase contamination can be serious problems. Research use only. Key application include reconstitution of lyophilized products and washing solution.
Deoxynucleotide Solution Mix (dNTP)
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New England Biolabs
Enhance your PCR Reaction with our ultrapure dNTPs
- Compatible with all NEB DNA polymerases in a variety of applications
- ≥ 99% pure as determined by HPLC analysis
- Exceptional stability
Deoxynucleotide Solution Set – 25 umol of each
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New England Biolabs
- Enhance your PCR reaction with our ultrapure dNTPs
- Compatible with all NEB DNA polymerases in a variety of applications
- Ultrapure: ≥ 99% as determined by HPLC analysis
- Exceptional stability
DNA Fingerprinting – Electrophoresis Education kit
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Cleaver Scientific
Our DNA fingerprinting kit is the perfect tool to introduce students to the world of electrophoresis and forensic science. DNA fingerprinting is a laboratory technique used to establish a link between biological evidence and a suspect in a criminal investigation. A DNA sample taken from a crime scene is compared with a DNA sample from a suspect. If the two DNA profiles are a match, then the evidence came from that suspect. Conversely, if the two DNA profiles do not match, then the evidence cannot have come from the suspect.
Each kit contains DNA, agarose gel, electrophoresis buffer and DNA stain enough for 8 groups of students to cast and run the experiment and visualise the DNA separation. The gel takes approximately 5 minutes to load, 30 minutes to run and 15 minutes to stain, meaning the whole experiment can be completed in a one hour lesson, with convenient incubation periods for scientific discussion and teaching. Students will solve a crime using an unknown DNA sample from the crime scene and a series of suspects!
Key Features
- Perfect for 1 hour lessons
- Introduce students to DNA and forensic science
- Works perfectly with Cleaver Scientific equipment
DNA Kit
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Cleaver Scientific
This simple kit will enable you to teach the principles behind gel electrophoresis. The kit contains five DNA samples labelled A to E, two of which are identical, with enough reagents to run two sets of six gels. The students will be able to run their own agarose gel and view the DNA after the samples have migrated through the gel. The DNA samples can be viewed on a standard UV transilluminator or using the totally sage blue light LED devices.
DNase I (RNase-free)
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New England Biolabs
- Endonuclease that nonspecifically cleaves DNA to release di-, tri- and oligonucleotide products with 5′-phosphorylated and 3′-hydroxylated ends
- Degrades DNA template in transcription reactions
- Removes contaminating genomic DNA from RNA samples
- Isolated from a recombinant source
