|Product Code||Description||Product Size||Quantity|
|CM0479B||ENDO AGAR BASE 500g||–||500g|
Endo Agar Base 500g
Thermo ScientificTM OxoidTM
A modified medium requiring the addition of basic fuchsin to form Endo Agar.
- Oxoid Endo Agar is an APHA recommended medium for the confirmation of coliforms.
- Also available Basic Fuchsin Indicator, Part No. BR0050A.
Endo Agar Base, Oxoid Composition
|pH 7.5 ± 0.2 @ 25°C|
Endo Agar, Oxoid Preparation:
Suspend 36g in 1 litre of distilled water. Add 6ml (or as directed by the supplier) of a 10% w/v solution of basic fuchsin BR0050 (50:50 ethanol/distilled water). Bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Mix well before pouring. Plates should be stored in the dark to preserve their pale pink colour.
BASIC FUCHSIN IS A POTENTIAL CARCINOGEN AND CARE SHOULD BE TAKEN TO AVOID INHALATION OF THE POWDERED DYE AND CONTAMINATION OF THE SKIN.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates at 2-8°C away from light.
Weigh out the basic fuchsin (BR0050) in a fume cupboard and avoid inhalation of the powder or contamination of the skin.
Keep the prepared medium away from light to avoid photo-oxidation.
Endo Agar is quoted by the American Public Health Association as a `Standard Methods’ medium for use in water1 and dairy products. Windle Taylor recommended the medium for the isolation and differentiation of coli-aerogenes bacteria from water.