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HomeCell Biology Minimum Essential Medium Eagle (EMEM) with Earle’s Salt, powder form
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Minimum Essential Medium Eagle (EMEM) with Earle’s Salt, powder form

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Sigma-Aldrich

Modification of original MEM formulation, which includes higher concentration of amino acids so the medium is more closely approximates the protein composition of cultured mammalian cells. EMEM has been used for wide variety of monolayer cells culture.

EMEM Modified with HEPES contains 25 mM HEPES to provide additional buffering capacity to the medium. A zwitterionic buffer, HEPES has a pKa of 7.3 at 37 °C, which is more compatible with most culture systems than that of sodium bicarbonate, which is usually 6.2 under similar conditions. HEPES will reduce sudden, drastic pH shifts, but as with other buffers, it will not prevent pH shifts entirely.

EMEM, which incorporates these modifications, includes 19 amino acids. The essential amino acids and the non-essential amino acids; L-ala; L-asn; L-asp; L-glu; L-gly; L-pro and L-ser. May be preferred for growth of adherent cells in the presence of reduced serum or as a base for development of a serum free medium.

Refer to the table below and choose your prefered EMEM!

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Description
Product CodeProduct NameNon-essential amino acidSodium PyruvatePhenol RedL-glutamineHEPESNaHCO3Storage Temperature
M0769-10X1LMinimum Essential Medium Eagle AutoMod™, with Earle’s salts, without L-glutamine and sodium bicarbonate, may autoclaveNoNoYesNoNoNo2-8°C
M2645-10LMinimum Essential Medium Eagle HEPES Modification, with Earle’s salts, L-glutamine and 25 mM HEPES, without sodium bicarbonateNoNoYesYes25mMNo2-8°C
M3024-10LMinimum Essential Medium Eagle with Earle’s salts and non-essential amino acids, without L-glutamine, phenol red and sodium bicarbonateYesNoNoNoNoNo2-8°C
M3024-10x1LMinimum Essential Medium Eagle with Earle’s salts and non-essential amino acids, without L-glutamine, phenol red and sodium bicarbonateYesNoNoNoNoNo2-8°C
M0643-10LMinimum Essential Medium Eagle With Earle’s salts, L-glutamine, and non-essential amino acids, without sodium bicarbonateYesNoYesYesNoNo2-8°C
M0643-10x1LMinimum Essential Medium Eagle With Earle’s salts, L-glutamine, and non-essential amino acids, without sodium bicarbonateYesNoYesYesNoNo2-8°C
M0268-10LMinimum Essential Medium Eagle With Earle’s salts and L-glutamine, without sodium bicarbonateNoNoYesYesNoNo2-8°C
M0268-10x1LMinimum Essential Medium Eagle With Earle’s salts and L-glutamine, without sodium bicarbonateNoNoYesYesNoNo2-8°C
Additional information
Weight40 kg
Dimensions20 × 40 × 100 cm
Brand

Sigma

Estimated Delivery Time

3 – 4 weeks

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Minimum Essential Medium Eagle (EMEM) with Earle's Salt, powder form

Minimum Essential Medium Eagle (EMEM) with Earle’s Salt, powder form

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