Dulbecco’s Modified Eagle’s Medium (DMEM), powder form
RM182.00 – RM381.00Brand:
Sigma-Aldrich
Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains four-fold concentrations of the amino acids and vitamins which suitable for cell culture. The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. Since then, it has been modified in several ways to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells. Each of these media offers a different combination of L-glutamine and sodium pyruvate. Refer to the table below and choose your prefered DMEM!
Minimum Essental Medium Eagle (EMEM) with Joklik modification, powder form
RM171.00 – RM212.00Brand:
Sigma-Aldrich
EMEM, which incorporates these modifications, includes higher concentrations of amino acids so the medium more closely approximates the protein composition of cultured mammalian cells. The formulation has been further modified by optional elimination of calcium to permit growth of cells in suspension culture.
Minimum Essential Medium Eagle (EMEM) with Alpha Modification, powder form
RM308.00 – RM491.00Brand:
Sigma-Aldrich
This is the most enriched variation of the EMEM formulation offered that suitable for cell culuture. It contains all 21 normal amino acids, some at increased concentrations. In addition, it contains 5 additional vitamins. Refer to the table below and choose your preferecd EMEM!
Minimum Essential Medium Eagle (EMEM) with Earle’s Salt, powder form
RM203.00 – RM372.00Brand:
Sigma-Aldrich
Modification of original MEM formulation, which includes higher concentration of amino acids so the medium is more closely approximates the protein composition of cultured mammalian cells. EMEM has been used for wide variety of monolayer cells culture.
EMEM Modified with HEPES contains 25 mM HEPES to provide additional buffering capacity to the medium. A zwitterionic buffer, HEPES has a pKa of 7.3 at 37 °C, which is more compatible with most culture systems than that of sodium bicarbonate, which is usually 6.2 under similar conditions. HEPES will reduce sudden, drastic pH shifts, but as with other buffers, it will not prevent pH shifts entirely.
EMEM, which incorporates these modifications, includes 19 amino acids. The essential amino acids and the non-essential amino acids; L-ala; L-asn; L-asp; L-glu; L-gly; L-pro and L-ser. May be preferred for growth of adherent cells in the presence of reduced serum or as a base for development of a serum free medium.
Refer to the table below and choose your prefered EMEM!
Minimum Essential Medium Eagle (EMEM) with Hanks’ salts
RM188.00Brand:
Sigma-Aldrich
Modification of the original MEM formulation. Earle’s salts have been replaced with Hanks’ salts. Since the bicarbonate concentration is substantially lower, this medium has a substantially reduced buffering capacity.
RPMI-1640 Medium
RM258.00 – RM438.00Brand:
Sigma-Aldrich
RPMI 1640 Medium, a modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells. Refer to the table below to choose the right RPMI-1640 for your cell culture!
