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2,4,6-Tris(2-pyridyl)-s-triazine, For Spectrophotometric Det. of Fe (Sigma-Aldrich)

RM624.00
Brand:

Sigma-Aldrich

Description:

CAS number: 3682-35-7
Chemical Formula: C18H12N6
Molar Mass: 312.33 g/mol
Synonyms: 2,4,6-Tri(2-pyridyl)-s-triazine, TPTZ

General description

2,4,6-Tris(2-pyridyl)-s-triazine is a Case and Koft compound generally used in the determination of iron in a colourimetric analysis. This reagent is for the spectrophotometric determination of Fe(II) and of total Fe.

3-Chloroperbenzoic acid, ≤77% (Sigma-Aldrich)

RM567.00
Brand:

Sigma-Aldrich

Description:

CAS number: 937-14-4
Chemical Formula: ClC6H4CO3H
Molar Mass: 172.57 g/mol
Synonyms: 3-Chloroperoxybenzoic acid, meta-Chloroperbenzoic acid, MCPBA

General description

3-Chloroperbenzoic acid is a strong oxidizing agent mainly used for the epoxidation of alkenes and also in the Baeyer-Villiger oxidation of ketones to esters.

Application

3-Chloroperbenzoic acid has been used in the oxidation of Np-tolylsulfinyl-(E)-1-phenylethylideneimine to Np-tolylsulfonyl (E)-1-phenylethylideneimine using dichloromethane as a solvent. It has also been used in the preparation of mono- and di-epoxy-functionalized poly(3-hydroxybutyrate)-based reactive polymers. MCPBA is also an effective oxidizing agent for the conversion of α-amide substituted polyesters to their corresponding polysulfones with tunable thermal properties.

Effective oxidant for epoxidizing di-, tri-, and tetra-substituted olefins.

4-Nitrophenyl butyrate, ≥98% (Sigma-Aldrich)

RM344.00
Brand:

Sigma-Aldrich

Description

CAS number: 2635-84-9
Chemical Formula: C10H11NO4
Molar Mass: 209.20 g/mol
Synonyms: p-Nitrophenyl butyrate, Butyric acid 4-nitrophenyl ester

General description

4-Nitrophenyl butyrate is a substrate for esterase, and lipase enzyme. Hydrolysis of 4-nitrophenyl butyrate by these enzymes releases the chromophore, 4-nitrophenolate which is spectrophotometrically analysed at 415 nm.

Application

4-nitrophenyl butyrate has been used:
• as a substrate for esterase activity in monocyte-derived macrophage cell line in response to polyethylene
• as a substrate for mycobacterial phospholipase A and mycobacterial cutinase activity
• as a substrate to determine the effect of buffers and solvents on human carboxylesterase

Benzyltriethylammonium Chloride, 99% (Sigma-Aldrich)

RM341.00RM827.00
Brand:

Sigma-Aldrich

General description

These durable TrueNorth® Cool Container with Lid are for chilling temperature-sensitive samples. They have heavy insulation properties for the ultracold and are ideal for ice as well as ice-salt slurries.

Features and Benefits

• made from single-moulded polyurethane
• close-fit lid keeps evaporation to a minimum and features a comfortable recessed knob
• lid nests under the bucket to save space
• for low-temperature use to -196ºC at 1 bar
• stackable
• pour spout and large handles

Butadiene sulfone, 98% (Sigma-Aldrich)

RM308.00
Brand:

Sigma-Aldrich

Description

CAS number: 77-79-2
Chemical Formula: C4H6O2S
Molar Mass: 118.15 g/mol
Synonyms: 2,5-Dihydrothiophene-1,1-dioxide, 3-Sulfolene

Chitosan, low molecular weight (Sigma-Aldrich)

RM1,265.00
Brand:

Sigma-Aldrich

Description:

CAS number: 9012-76-4
Synonyms: Deacetylated chitin, Poly(D-glucosamine)

General description

Chitosan may be used as a flocculant, in protein precipitation, encapsulating agent and aqueous thickener.

Forms gels with multivalent anions. Gives clear solutions that dry to strong, clear films.

Features and Benefits

Biocompatible, antibacterial and environmentally friendly polyelectrolyte with a variety of applications including water treatment, chromatography, additives for cosmetics, textile treatment for antimicrobial activity, novel fibres for textiles, photographic papers, biodegradable films, biomedical devices, and microcapsule implants for controlled release in drug delivery.

Chlorpyrifos-methyl, PESTANAL®, analytical standard (Sigma-Aldrich)

RM186.00
Brand:

Sigma-Aldrich

Application

HPLC: suitable
Gas Chromatography (GC): suitable

Cholesterol Quantitation Kit (Sigma-Aldrich)

RM2,705.00
Brand:

Sigma-Aldrich

Description

Synonym: Cholesterol Assay Kit

General description

Cholesterol is an important component of mammalian cell membranes where it functions in intracellular transport, cell signalling, and maintaining membrane fluidity. Within the blood, cholesterol circulates as both the free acid and as cholesterol esters. Controlling serum cholesterol has an important therapeutic role as elevated cholesterol levels are associated with the development of atherosclerosis and cardiovascular pathologies. Recent evidence suggests a disturbance of cholesterol homeostasis contributes to the development of a chronic inflammatory state.

Cholesterol is predominantly produced in the liver. Abnormal cholesterol levels are implicated in coronary heart disease. Cholesterol is associated with breast cancer progression or inhibition. Tumour cells are capable of synthesizing their own cholesterol, which aids in cell proliferation. Increased cholesterol level is observed in cancers, especially in prostate cancer. Elevated serum cholesterol is observed in obesity.

Application

Cholesterol Quantitation Kit has been used to measure the total serum cholesterol level.

Suitability

Suitable for use with cell and tissue culture samples, urine, plasma, serum, and other biological samples.

Principle

The Cholesterol Quantitation Kit can also be used to determine the concentration of free cholesterol, cholesteryl esters, or both (total) present in a sample. In this kit, total cholesterol concentration is determined by a coupled enzyme assay, which results in a colorimetric (570 nm)/fluorometric (λex = 535/λem = 587 nm) product, proportional to the cholesterol present.

Cycloheximide from microbial, ≥94% (TLC) (Sigma-Aldrich)

RM348.00
Brand:

Sigma-Aldrich

Description

CAS number: 66-81-9
Chemical Formula: C15H23NO4
Molar Mass: 281.35 g/mol
Synonyms: 3-[2-(3,5-Dimethyl-2-oxocyclohexyl)-2-hydroxyethyl]glutarimide, Actidione, Naramycin A

Application

In yeast strains, cycloheximide has been used as a protein synthesis inhibitor in the cycloheximide chase experiment. It has been used to inhibit translation in mammalian cells and suppress fungal growth.

Biochem/physiol Actions

Cycloheximide (CHX) is an antibiotic produced by S. griseus. Its main biological activity is translation inhibition in eukaryotes resulting in cell growth arrest and cell death. CHX is widely used for selection of CHX-resistant strains of yeast and fungi, controlled inhibition of protein synthesis for detection of short-lived proteins and super-induction of protein expression, and apoptosis induction or facilitation of apoptosis induction by death receptors.

Cytochrome C from Bovine Heart (Sigma-Aldrich)

RM721.00
Brand:

Sigma-Aldrich

Description

CAS number: 9007-43-6

Application

Cytochrome c has been identified as an important mediator in apoptotic pathways. The release of mitochondrial cytochrome c into the cytoplasm stimulates apoptosis and is commonly used as an indicator of the apoptotic process in the cell. Investigation on the effect of Paris Saponin I (PS I) on human gastric carcinoma cell growth (SGC7901 cells) have shown an elevated level cytoplasmic cytochrome c. Results are inhibition of proliferation in SGC7901 cells by inducing mitochondria-dependent apoptosis through cytochrome c.

Biochem/physiol Actions

The ready interconversion of cytochrome c between ferrous and ferric states makes it an efficient biological electron carrier. It plays a vital role in cellular oxidations in both plants and animals. Generally regarded as a universal link in the respiratory chain, it forms the essential electron-bridge between the respirable substrates and oxygen.

Dichloromethane, HPLC Plus (Sigma-Aldrich)

RM526.00
Brand:

Sigma-Aldrich

Description

CAS number: 75-09-2
Chemical Formula: CH2Cl2
Molar Mass: 84.93 g/mol
Synonyms: Methylene chloride, methylene chloride

Application

Suitable for HPLC, GC, spectrophotometry, environmental testing and residue analysis.

Dimethyl Sulfoxide, ≥99.7% (Sigma-Aldrich)

RM398.00
Brand:

Sigma-Aldrich

Description

CAS number: 67-68-5
Chemical Formula: (CH3)2SO
Molar Mass: 78.13 g/mol
Sterility: Sterile-filtered
Synonyms: DMSO

Application

DMSO is a polar aprotic solvent used in chemical reactions, in polymerase chain reactions (PCR) and as a cryoprotectant vitrification agent for the preservation of cells, tissues and organs. DMSO is used in cell freezing media to protect cells from ice crystal-induced mechanical injury. It is used for frozen storage of primary, sub-cultured, and recombinant heteroploid and hybridoma cell lines; embryonic stem cells (ESC), and hematopoietic stem cells. DMSO is frequently used in the combinations with BSA or fetal bovine serum (FBS).

Other Notes

This product is a Hybri-Max product. It is hybridoma tested and is assessed for suitability in cell freezing. This product is sterile filtered (0.2 micron) and tested for endotoxin levels.

Dimethyl Sulfoxide, Anhydrous, ≥99.9% (Sigma-Aldrich)

RM689.00RM804.00
Brand:

Sigma-Aldrich

Description

CAS number: 67-68-5
Linear Formula: (CH3)2SO
Molecular Weight 78.13
Synonym: DMSO

General description

Dimethyl Sulfoxide (DMSO) is an apolar protic solvent that is generally used as a reaction medium and reagent in organic reactions.

Application

Dimethyl Sulfoxide may be used as an oxidant for the conversion of isonitriles into isocyanates. DMSO activated by oxalyl chloride can be used in the oxidation of long-chain alcohols to carbonyls.

Dimidium bromide, BioReagent, suitable for fluorescence, ~95% (AT), Sigma

RM700.00
Brand:

Sigma-Aldrich

CAS number: 518-67-2
Chemical Formula: C20H18BrN3
Molar Mass: 380.28 g/mol
Synonyms: 3,8-Diamino-5-methyl-6-phenylphenanthridinium bromide, Trypadine

Application

Intercalating probe for nucleic acids. Suitable for fluorescence.

Dulbecco’s Modified Eagle’s Medium (DMEM), powder form

RM182.00RM381.00
Brand:

Sigma-Aldrich

Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains four-fold concentrations of the amino acids and vitamins which suitable for cell culture. The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. Since then, it has been modified in several ways to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells. Each of these media offers a different combination of L-glutamine and sodium pyruvate. Refer to the table below and choose your prefered DMEM!

Dulbecco’s Modified Eagle’s Medium (DMEM), powder form Brochure

Glutathione, Certified Reference Material (Sigma-Aldrich)

RM413.00
Brand:

Sigma-Aldrich

Description

CAS number: 70-18-8
Chemical Formula: H2NCH(CO2H)CH2CH2CONHCH(CH2SH)CONHCH2CO2H
Molar Mass: 307.32g/mol
Synonyms: γ-L-Glutamyl-L-cysteinyl-glycine, L-Glutathione reduced, GSH

Amino Acid Sequence

γ-Glu-Cys-Gly

General description

Glutathione (GSH) is the most important nonprotein thiol widely distributed in animal tissues, plants, and microorganisms. GSH is also a key determinant of redox signalling and protection against oxidative stress.
Pharmaceutical secondary standards for application in quality control, provide pharma laboratories and manufacturers with a convenient and cost-effective alternative to the preparation of in-house working standards.

Application

HPLC: suitable
Gas Chromatography (GC): suitable

These Secondary Standards are qualified as Certified Reference Materials. These are suitable for use in several analytical applications including but not limited to pharma release testing, pharma method development for qualitative and quantitative analyses, food and beverage quality control testing, and other calibration requirements.

May be used at 5-10 mM to elute glutathione S-transferase (GST) from glutathione agarose.

Biochem/physiol Actions

Endogenous antioxidant that plays a major role in reducing reactive oxygen species formed during cellular metabolism and the respiratory burst. Glutathione-S-transferase catalyzes the formation of glutathione thioethers with xenobiotics, leukotrienes, and other molecules that have an electrophilic center. Glutathione also forms disulfide bonds with cysteine residues in proteins. Via these mechanisms, it can have the paradoxical effect of reducing the efficacy of anti-cancer agents.

 

Glycerol, For Molecular Biology, ≥99.0% (Sigma-Aldrich)

RM460.00
Brand:

Sigma-Aldrich

Description

CAS number: 56-81-5
Chemical Formula: HOCH2CH(OH)CH2OH
Molar Mass: 92.09 g/mol
Synonyms: 1,2,3-Propanetriol, Glycerin

General description

Glycerol is odourless, colourless, viscous in nature, and exists as a sweet-tasting liquid. It can be derived naturally as well as from petrochemical feedstock. Glycerol has a wide variety of applications and is one of the most valuable and versatile chemical substances in nature. It can be used as an emollient, solvent, sweetening agent, in pharmaceutical formulations, cosmetics, foodstuffs and toiletries. It is very stable and can be easily stored under normal temperature; also it is non-irritating and has no adverse impact on the environment.

Application

Glycerol has been used as:
• A supplement during cell culture of Mycobacterium tuberculosis and Mycobacterium avium.
• A fuel during designing enzymatic biofuel cell.
• A liquid composite matrix with 4-HCCA and 3-amino­quinoline for analysis of neutral and acidic glycans.
• A matrix for fast atom bombardment MS.
• May be employed as liquid matrix for the quantification studies by MALDI (Matrix-assisted laser desorption/ionization mass spectrometry) analysis.

Glycerol is used both in sample preparation and gel formation for polyacrylamide gel electrophoresis. Glycerol (5-10%) increases the density of a sample so that the sample will layer at the bottom of a gel′s sample well. Glycerol is also used to aid in casting gradient gels and as a protein stabilizer and storage buffer component.

Glycerol is used both in sample preparation and gel formation for polyacrylamide gel electrophoresis. Glycerol (5-10%) increases the density of a sample so that the sample will layer at the bottom of a gel’s sample well. Glycerol is also used to aid in casting gradient gels and as a protein stabilizer and storage buffer component.

Histopaque®-1077 (Sigma-Aldrich)

RM286.00
Brand:

Sigma-Aldrich

General description

Histopaque-1077 is a sterile, endotoxin tested solution of polysucrose and sodium diatrizoate, adjusted to a density of 1.077g/mL. This ready-to-use medium facilitates rapid recovery of viable lymphocytes and other mononuclear cells from small volumes of whole blood.

Application

Haematology: suitable
Histology: suitable

Histopaque-1077 has been used for-
• the isolation of peripheral blood mononuclear cells (PBMCs) and bone marrow mononuclear cells (BMMCs)
• purification of mononuclear cells through centrifugation
• isolation of human lymphocytes
• isolation of breast cancer circulating tumour cells (CTCs) from whole blood

Litmus Milk, for Microbiology, NutriSelect™ Plus, Sigma

RM365.00
Brand:

Sigma-Aldrich

Application

For maintenance of Lactobacilli and for determining the action of bacteria on milk.

Typical Formula *
gm/litre
Litmus

0.5

Skim Milk Powder

100

Sodium Sulfite

0.5

Final pH: 6.8±0.2 (25 °C)

 

Methyl cellulose, Viscosity: 400 cP (Sigma-Aldrich)

RM280.00
Brand:

Sigma-Aldrich

Description

CAS number: 9004-67-5

General description

Methyl-cellulose powder helps to prevent and manage nasal symptoms.

Application

Methyl cellulose has been used:
• in total gastrointestinal transit
• in in vitro motility assay
•  in the measurement of gastric emptying by phenol red method

Minimum Essental Medium Eagle (EMEM) with Joklik modification, powder form

RM171.00RM212.00
Brand:

Sigma-Aldrich

EMEM, which incorporates these modifications, includes higher concentrations of amino acids so the medium more closely approximates the protein composition of cultured mammalian cells. The formulation has been further modified by optional elimination of calcium to permit growth of cells in suspension culture.

Minimum Essental Medium Eagle (EMEM) with Joklik modification, powder form Brochure

Minimum Essential Medium Eagle (EMEM) with Alpha Modification, powder form

RM308.00RM491.00
Brand:

Sigma-Aldrich

This is the most enriched variation of the EMEM formulation offered that suitable for cell culuture. It contains all 21 normal amino acids, some at increased concentrations. In addition, it contains 5 additional vitamins. Refer to the table below and choose your preferecd EMEM!

Minimum Essential Medium Eagle (EMEM) with Alpha Modification, powder form Brochure

Minimum Essential Medium Eagle (EMEM) with Earle’s Salt, powder form

RM203.00RM372.00
Brand:

Sigma-Aldrich

Modification of original MEM formulation, which includes higher concentration of amino acids so the medium is more closely approximates the protein composition of cultured mammalian cells. EMEM has been used for wide variety of monolayer cells culture.

EMEM Modified with HEPES contains 25 mM HEPES to provide additional buffering capacity to the medium. A zwitterionic buffer, HEPES has a pKa of 7.3 at 37 °C, which is more compatible with most culture systems than that of sodium bicarbonate, which is usually 6.2 under similar conditions. HEPES will reduce sudden, drastic pH shifts, but as with other buffers, it will not prevent pH shifts entirely.

EMEM, which incorporates these modifications, includes 19 amino acids. The essential amino acids and the non-essential amino acids; L-ala; L-asn; L-asp; L-glu; L-gly; L-pro and L-ser. May be preferred for growth of adherent cells in the presence of reduced serum or as a base for development of a serum free medium.

Refer to the table below and choose your prefered EMEM!

Minimum Essential Medium Eagle (EMEM) with Hanks’ salts

RM188.00
Brand:

Sigma-Aldrich

Modification of the original MEM formulation. Earle’s salts have been replaced with Hanks’ salts. Since the bicarbonate concentration is substantially lower, this medium has a substantially reduced buffering capacity.

Minimum Essential Medium Eagle (EMEM) with Hanks’ salts Brochure