RPMI-1640 Medium with 20 mM HEPES and L-glutamine, Sigma – Aldrich
Synonyms: Roswell Park Memorial Institute 1640 medium
Techniques: Cell culture | mammalian: suitable
Impurities: Endotoxin Tested
HEPES: 20 mM
sodium pyruvate: no
phenol red: yes
RPMI 1640 Medium is a modification of McCoy’s 5A Medium designed to support lymphoblastoid cells in suspension culture. Although it was originally formulated to be used with a serum supplement, it has since been demonstrated to be capable of supporting a wide range of anchorage-dependent cells. RPMI 1640 Medium has also been extensively used in hybrid cell growth and fusion protocols. It has been shown to be suitable for the culturing of human normal and neoplastic leukocytes. This medium is based on the RPMI-1630 series of media and uses a bicarbonate buffering system with adjustments to amino acid and vitamin levels. It is also used for the growth of various cell cultures, such as fresh human lymphocytes in the 72-hour phytohemagglutinin (PHA) stimulation assay.