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HomeMicrobiologyCulture Media Products Raka-Ray Medium 500g
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oxoid

Raka-Ray Medium 500g

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Brand:

Thermo ScientificTM OxoidTM

  • Oxoid Raka-Ray Agar Base is a medium for the isolation of lactic acid bacteria in beer and brewing processes.
  • Requires supplementation with phenylethanol, sorbitan mono-oleate, and cycloheximide Part No. SR0222C.

Raka-Ray Agar Base, Oxoid Composition

Typical Formula*

gm/litre

Yeast extract

5.0

Tryptone

20.0

Liver concentrate

1.0

Maltose

10.0

Fructose

5.0

Glucose

5.0

Betaine HCL

2.0

Diammonium hydrogen-citrate

2.0

L-Aspartic acid†

2.5

L-Glutamic acid††

2.5

Magnesium sulphate. 7H2O

2.0

Manganese (II) sulphate. 4H2O

0.66

Potassium phosphate

2.0

N-Acetyl glucosamine

0.5

Agar

17.0

pH 5.4 ± 0.2 @ 25°C

 

Raka-Ray Agar, Oxoid Preparation:
Suspend 77.1g in 1 litre of distilled water. Add 10ml of sorbitan mono-oleate and 7ml of 0.1% Cycloheximide Solution (SR0222). Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50-55°C and aseptically add 3g of phenylethanol. Pour into sterile Petri dishes or distribute into 4ml volumes held at 55°C if the overlay technique is to be used.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
Although the concentration of cycloheximide in the medium is below toxic levels, precautions should be observed as detailed under HAZARDS section.

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SKU: CM0777B Categories: Culture Media Products, Microbiology
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  • Description
  • Additional information
Description
Product CodeDescriptionProduct SizeQuantity
CM0777BRaka-Ray Medium 500g–500g
Additional information
Dimensions10 cm
Brand

Oxoid

Estimated Delivery Time

1-2 weeks

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Raka-Ray Medium 500g

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