|MLCB Agar 500g
MLCB Agar 500g
Thermo ScientificTM OxoidTM
Oxoid MLCB Agar is used for the selective isolation of Salmonella spp., not S.Typhi or S. Paratyphi A.
MLCB Agar, Oxoid Composition
|Ammnium iron (III) citrate
|pH 6.8 ± 0.2 @ 25°C
MLCB Agar, Oxoid Preparation:
Suspend 49.0g in 1 litre of distilled water. Mix and bring gently to the boil with frequent agitation to dissolve the medium completely. Cool to 50°C and pour approximately 20ml into sterile Petri dishes.
DO NOT AUTOCLAVE OR OVERHEAT.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates of medium at 2-8°C.
The identity of colonies presumed from their appearance to be Salmonella spp. must be confirmed by biochemical and serological testing. In common with other enteric media care must be taken to ensure the purity of colonies taken for further testing as organisms that are inhibited from developing into colonies remain viable and may accidentally be picked on sub-culture.