Product Code | Description | Product Size | Quantity | Format |
CM0995B | Burkholderia Cepacia Agar Base 500g | – | 500g | Powder |
Burkholderia Cepacia Agar Base 500g
Brand:
Thermo ScientificTM OxoidTM
Isolate Burkholderia cepacia from respiratory secretions of patients with cystic fibrosis with Thermo Scientific™ Oxoid™ Burkholderia Cepacia Agar Base, which is also used for routine testing of non-sterile inorganic salt solutions in hospital environments. The slower growing B. cepacia can be missed on conventional media, such as blood or MacConkey Agar, due to overgrowth caused by other faster growing organisms found in the respiratory tract of CF patients, such as mucoid Klebsiella species,
Pseudomonas aeruginosa and Staphylococcus species.This may lead to the infection being missed or wrongly diagnosed.
Burkholderia Cepacia Agar Base, Oxoid Composition
Typical Formula* | gm/litre |
Peptone | 5.0 |
Yeast Extract | 4.0 |
Sodium pyruvate | 7.0 |
Potassium dihydrogen phosphate | 4.4 |
Disodium hydrogen phosphate | 1.4 |
Bile salts | 1.5 |
Ammonium sulphate | 1.0 |
Magnesium sulphate | 0.2 |
Ammonium ferrous sulphate | 0.01 |
Phenol red | 0.02 |
Crystal violet | 0.001 |
Agar | 12.0 |
pH 6.2 ± 0.2 @ 25°C |
Burkholderia Cepacia Agar, Oxoid Preparation:
Suspend 18.25g of Burkholderia cepacia Agar Base in 500ml of distilled water, mix well and sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C and aseptically add the contents of one vial of Burkholderia cepacia Selective Supplement SR0189, reconstituted as directed. Mix well and distribute into sterile Petri dishes.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates of medium at 2-8°C.
Dimensions | 10 cm |
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Brand | Oxoid |
Estimated Delivery Time | 1-2 weeks |