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Lactose Broth 500g

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Brand:

Thermo ScientificTM OxoidTM

Presumptively identify coliform organisms in milk, water and foods as specified by the American Public Health Association with Thermo Scientific™ Oxoid™ Lactose Broth (Dehydrated). Beef extracts and peptone in this liquid medium supply essential nutrients while lactose is a fermentable carbohydrate for coliforms. Coliforms ferment lactose in the medium resulting in gas formation which is indicative of positive results.

Lactose Broth, Oxoid Composition

Typical Formula

gm/litre

`Lab-Lemco’ powder

3.0

Peptone

5.0

Lactose

5.0

pH 6.9 ± 0.2 @ 25°C

 

Lactose Broth, Oxoid Preparation:
Dissolve 13g in 1 litre of distilled water and distribute into containers with fermentation tubes (Durham). Sterilise by autoclaving at 121°C for 15 minutes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at room temperature (18-22°C).

Precautions
Ensure that the fermentation tubes are free from air bubbles before inoculation.
Large water samples may require double-strength lactose broth to reduce the final volumes. Do not overheat double- strength broth or inhibitory products will be produced.

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SKU: CM0137B Categories: Culture Media Products, Microbiology
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  • Description
  • Additional information
Description
Product CodeDescriptionProduct SizeQuantity
CM0137BLACTOSE BROTH 500g–500g
Additional information
Dimensions10 cm
Brand

Oxoid

Estimated Delivery Time

1-2 weeks

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Lactose Broth 500g

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