|MYCOPLASMA BROTH BASE 500g
Mycoplasma Broth Base 500g
Thermo ScientificTM OxoidTM
- Oxoid Mycoplasma Broth Base is used for the selective isolation of Mycoplasma species.
- Also available Mycoplasma Supplement G, Part No. SR0059C.
Mycoplasma Broth Base, Oxoid Composition
|pH 7.8 ± 0.2 @ 25°C
Mycoplasma Broth, Oxoid Preparation:
Dissolve 25.5g in 1 litre of distilled water. Mix well and distribute in 80ml volumes. Sterilise by autoclaving at 121°C for 15 minutes. Cool to approximately 50°C and add one vial of Mycoplasma Supplement SR0059 reconstituted as directed.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.
Thallous acetate is toxic, observe the precautions stated under Hazards section..
Sub-culture the broth to agar as soon as any indicator added begins to change colour before the pH change destroys the organism.