Mueller Hinton Broth 500g, Oxoid

Mueller Hinton Broth, Oxoid conforms to international standards for antimicrobial susceptibility testing media.

Mueller Hinton Broth, Oxoid Preparation
Place 21.0g of Mueller Hinton Broth, Oxoid in 1 litre of distilled water mix to dissolve completely. Sterilize by autoclaving at 121°C for 15 minutes. Chill and adjust cation levels if necessary.

Storage conditions and Shelf life
Store the dehydrated Mueller Hinton broth at 10-30°C and use before the expiry date on the label.
Store the prepared Mueller Hinton broth at 2-8°C.

Precautions
Monitor the performance of the broth routinely using the standard QC organisms. If the broth does not yield the expected MIC values, modify the volumes of Mg++ and Ca++ solutions.

If the thymidine content is lowered, after the addition of lysed horse blood or thymidine phosphorylase, the MIC values may be lower.