|MUELLER HINTON BROTH 500g
Mueller Hinton Broth 500g, Oxoid
Thermo ScientificTM OxoidTM
Mueller Hinton Broth, Oxoid conforms to international standards for antimicrobial susceptibility testing media.
|Dehydrated infusion from Beef
|pH 7.3 ± 0.1 @ 25°C
Mueller Hinton Broth, Oxoid Preparation
Place 21.0g of Mueller Hinton Broth, Oxoid in 1 litre of distilled water mix to dissolve completely. Sterilize by autoclaving at 121°C for 15 minutes. Chill and adjust cation levels if necessary.
Storage conditions and Shelf life
Store the dehydrated Mueller Hinton broth at 10-30°C and use before the expiry date on the label.
Store the prepared Mueller Hinton broth at 2-8°C.
Monitor the performance of the broth routinely using the standard QC organisms. If the broth does not yield the expected MIC values, modify the volumes of Mg++ and Ca++ solutions.
If the thymidine content is lowered, after the addition of lysed horse blood or thymidine phosphorylase, the MIC values may be lower.