|MYP Agar 500g
MYP Agar 500g
Thermo ScientificTM OxoidTM
Selectively enumerate Bacillus cereus in food samples with Thermo Scientific™ Oxoid™ MYP Agar (Dehydrated). The medium relies upon mannitol fermentation and phospholipase C production to differentiate Bacillus cereus from other members of the species.
Mannitol-negative Bacillus cereus form pink colored colonies due to the presence of Phenol Red Indicator. Egg yolk emulsion helps in the differentiation of phospholipase C producing colonies, which are surrounded by a zone of the white precipitate. Polymixin B Sulphate acts as the inhibitor to restrict the growth of Gram-negative bacteria.
MYP Agar, Oxoid Composition
|pH 7.2 ± 0.2 @ 25°C
MYP Agar, Oxoid Preparation:
Suspend 21.5g in 450ml distilled water and bring gently to the boil to dissolve. Sterilise by autoclaving at 121°C for 15 minutes.
Cool to approximately 49°C and aseptically add 50ml Egg Yolk Emulsion SR0047 and 1 vial of Polymyxin B Supplement SR0099, reconstituted as directed. Mix well and pour into sterile petri dishes.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates at 2-8°C.