|Sorbitol MacConkey Agar 500g
Sorbitol MacConkey Agar 500g
Thermo ScientificTM OxoidTM
- Oxoid Sorbitol MacConkey Agar is a selective differential medium for the detection of E. coli O157.
- Also available C-T Supplement, Part No. SR0172E and SR0172H.
Sorbitol MacConkey Agar Base, Oxoid Composition
|Bile salts No.3
|pH 7.1 ± 0.2 @ 25°C
Sorbitol MacConkey Agar, Oxoid Preparation:
Suspend 51.5g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Allow to cool to 50°C. Pour into sterile Petri dishes.
If required, reconstitute one vial of C-T supplement per 500ml medium, according to the instructions in the product leaflet. Aseptically add this to cooled medium before pouring into sterile Petri dishes
Storage conditions and Shelf life
Dehydrated Sorbitol MaConkey Agar must be stored tightly capped in the original container at 10-30ºC.
Oxoid Sorbitol MacConkey and Cefixime Tellurite Sorbitol MacConkey plates should be stored in the original packaging, at the temperature stated on the pack or product specification, and protected from direct light. When stored as directed, the unopened product will remain stable until the expiry date on the label.
Locally prepared media can be stored for up to 2 weeks when made from CM0813 and SR0172 according to the manufacturer’s instructions and stored at 2-8ºC, out of direct sunlight and protected from dessication. A longer shelf life may be attainable, but should be validated under the relevant, local manufacturing and storage conditions.