| Product Code | Description | Product Size | Quantity |
| CM0817B | M17 Broth 500g | – | 500g |
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E.C. Broth 500g RM390.00
M17 Broth 500g
RM497.00
Brand:
Thermo ScientificTM OxoidTM
Cultivate lactic streptococci and their bacteriophages with Thermo Scientific™ Oxoid™ M17 Broth (Dehydrated). The use of M17 Broth in conjunction with M17 Agar is recommended by Terzaghi and Sandine for bacteriophage assays.
M17 Broth, Oxoid Composition
| Typical Formula* | gm/litre |
| Tryptone | 5.0 |
| Soya peptone | 5.0 |
| `Lab-Lemco’ powder | 5.0 |
| Yeast extract | 2.5 |
| Ascorbic acid | 0.5 |
| Magnesium sulphate | 0.25 |
| Di-sodium-glycerophosphate | 19.0 |
| pH 6.9 ± 0.2 @ 25°C |
M17 Broth, Oxoid Preparation:
Suspend 37.25g in 950ml of distilled water and bring gently to the boil. Sterilize by autoclaving at 121°C for 15 minutes. Cool to 50°C and aseptically add 50ml of sterile lactose solution (10% w/v).
Lactose solution 10% (w/v):
Dissolve 10g of Lactose Code LP0070 in 100ml of distilled water. Sterilise by autoclaving at 121°C for 15 minutes or by membrane filtration through a 0.2mm membrane.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.
100% Authentic Guarantee Description
Additional information
| Dimensions | 10 cm |
|---|---|
| Brand | Oxoid |
| Estimated Delivery Time | 1-2 weeks |
