High-Performance Liquid Chromatography (HPLC) is a method used nowadays to separate the mixture under high pressure. Usually, the sample with a suitable solvent (mobile phase) injected into an HPLC column filled with packed particles (stationary phase). When the mobile phase passes through the column, each component in the sample starts to separate and elute at different time.

What are the factors affecting HPLC column suitability?

Many factors are affecting the HPLC column suitability, including Types of HPLC Columns, Length and Internal Diameter, Bead Size and Pore Size

Types of HPLC Column.

most commonly used types are Normal-Phase HPLC Columns, Reverse-Phase HPLC Columns, Ion-Exchange HPLC Columns, Size Exclusion HPLC Columns, Affinity HPLC Columns, etc.