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4D-Nucleofector™ System

RM0.00
Brand:

Lonza

Description
The 4D-Nucleofector™ System was developed to offer advanced performance, flexibility and convenience. The system is controlled by intuitive software on a touch screen user interface. Its modular architecture allows seamless expansion of the system for new applications. It comprises a core unit and different functional units:

The Core Unit – Controlling the 4D-Nucleofector™ System

  • 5.7’’ foldable touch screen to operate the system and controlling the functional units
  • Intuitive operation software for designing and saving individual experimental setups
  • USB port for software update and data transfer

The X unit – Supporting Nucleofection™ of various cell numbers in different formats

  • Features positions for 20µl Nucleocuvette™ strips and 100 μl single Nucleocuvette™
  • Seamless transfer of conditions between different Nucleofection™ vessels
  • Comprises HV connectivity for the 96-well Shuttle™ Device

The Y unit – Enabling adherent Nucleofection™ in 24-well culture plates

  • Nucleofection™ of neurons in adherence in any developmental stage
  • Up to 70% efficiency and maintenance of functionality
  • Suitable for confocal microscopy read-out

4D-Nucleofector™ System Brochure

Please contact us for pricing and availability.

CytoSMART™ 2 System

(1 customer review)
RM0.00
Brand:

Lonza

Description

The CytoSMART™ 2 System is designed for bright-field live cell imaging for applications, such as general cell culture monitoring, documentation and scratch/migration assays, which require a larger field of view. The field of view is 2.4 x 1.4 mm and the magnification is similar to that typically achieved with a 10X objective using a conventional microscope.

The device features an improved optical system and more powerful camera unit, advancing the image resolution. Images can be digitally enlarged two-fold, displaying more cellular details.Image capture and recording time-lapse videos of cell cultures are easily performed with the intuitive CytoSMART™ App. Monitoring of cell cultures outside of the lab can be done anytime and anywhere, via smart phone, tablet or computer with the integrated cloud functionality. Data transfer to the cloud via Wifi or LAN transmission, as well as offline operation, allow flexible data recording and storage for different network environments.

The CytoSMART™ 2 System is operated via a CytoSMART™ Tablet. The tablet can be controlled by either the on-screen keyboard or the keyboard base attached to it. It supports operating the CytoSMART™ 2 System in an environment where wearing gloves is mandatory.

The CytoSMART™ 2 System offers an extra cell counting feature that determines the number of cells in suspension in a standard hemocytometer.

CytoSMART™ 2 System Brochure

Please contact us for pricing and availability.

DMEM with High Glucose with Glutamine without Sodium Pyruvate 500ml

RM327.00
Brand:

Lonza

Product Overview

DMEM is a modification of Basal Medium Eagle (BME) that contains four-times the concentrations of the amino acids and vitamins.

The formulation first contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. DMEM media is suitable for most types of cells, including human, monkey, hamster, rat, mouse, chicken and fish cells.The high glucose version is well suited to high density suspension culture.

DMEM will work with different cell lines, it can also be used with primary cells such as fibroblasts.

Shipping: Ambient

Storage: 2-8° Celsius

Shelf life: 2 years from date of manufacture

All BioWhittakerTM Cell Culture Media products are for Research Use Only (RUO) and are not approved for human or veterinary use or for use in clinical or in vitro diagnostic procedures. If you require GMP grade media, contact us for more details.

DMEM with High Glucose with Glutamine without Sodium Pyruvate Brochure

Content

  • 500ml bottle

DMEM with High Glucose with L-Glutamine 1L

RM367.00
Brand:

Lonza

Product Overview

DMEM is a modification of Basal Medium Eagle (BME) that contains four-times the concentrations of the amino acids and vitamins.

The formulation first contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. DMEM media is suitable for most types of cells, including human, monkey, hamster, rat, mouse, chicken and fish cells. The high glucose version is well suited to high density suspension culture.

DMEM will work with different cell lines, it can also be used with primary cells such as fibroblasts.

Shipping: Ambient Storage: 2-8° Celsius

Shelf life: 2 years from date of manufacture

All BioWhittakerTM Cell Culture Media products are for Research Use Only (RUO) and are not approved for human or veterinary use or for use in clinical or in vitro diagnostic procedures. If you require GMP grade media, contact us for more details.

DMEM with High Glucose with L-Glutamine Brochure

Content

  • 1L bottle
  • with L-glutamine
  • with Sodium Pyrovate
  • with Phenol Red
  • without HEPES
  • with 4.5 g/L glucose

DMEM with High Glucose with L-Glutamine 500ml

RM331.00
Brand:

Lonza

Product Overview

DMEM is a modification of Basal Medium Eagle (BME) that contains four-times the concentrations of the amino acids and vitamins. The formulation first contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. DMEM media is suitable for most types of cells, including human, monkey, hamster, rat, mouse, chicken and fish cells. The high glucose version is well suited to high density suspension culture.

DMEM will work with different cell lines, it can also be used with primary cells such as fibroblasts.

Shipping: Ambient Storage: 2-8° Celsius

Shelf life: 2 years from date of manufacture

All BioWhittakerTM Cell Culture Media products are for Research Use Only (RUO) and are not approved for human or veterinary use or for use in clinical or in vitro diagnostic procedures. If you require GMP grade media, contact us for more details.

DMEM with High Glucose with L-Glutamine Brochure

Content

  • 500 ml bottle
  • with L-glutamine
  • with Sodium Pyrovate
  • with Phenol Red
  • without HEPES
  • with 4.5 g/L glucose

DMEM with High Glucose with UltraGlutamine 500ml

RM333.00
Brand:

Lonza

Product Overview
DMEM is a modification of Basal Medium Eagle (BME) that contains four-times the concentrations of the amino acids and vitamins.

The formulation first contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. DMEM media is suitable for most types of cells, including human, monkey, hamster, rat, mouse, chicken and fish cells. The high glucose version is well suited to high density suspension culture.

DMEM will work with different cell lines, it can also be used with primary cells such as fibroblasts.

Shipping: Ambient Storage: 2-8° Celsius

Shelf life: 2 years from date of manufacture

All BioWhittakerTM Cell Culture Media products are for Research Use Only (RUO) and are not approved for human or veterinary use or for use in clinical or in vitro diagnostic procedures. If you require GMP grade media, contact us for more details.

DMEM with High Glucose with UltraGlutamine Brochure

Content

  • 500 ml bottle

DMEM with High Glucose without L-Glutamine without Phenol Red 500ml

RM386.00
Brand:

Lonza

Product Overview

DMEM is a modification of Basal Medium Eagle (BME) that contains four-times the concentrations of the amino acids and vitamins.

The formulation first contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. DMEM media is suitable for most types of cells, including human, monkey, hamster, rat, mouse, chicken and fish cells.The high glucose version is well suited to high density suspension culture.

DMEM will work with different cell lines, it can also be used with primary cells such as fibroblasts.

All BioWhittakerTM Cell Culture Media products are for Research Use Only (RUO) and are not approved for human or veterinary use or for use in clinical or in vitro diagnostic procedures. If you require GMP grade media, contact us for more details.

DMEM with High Glucose with Glutamine without Sodium Pyruvate Brochure

Content

  • 500 ml Bottle
  • without L-glutamine
  • with Sodium pyruvate
  • without Phenol Red
  • With 4.5 g/L Glucose

DMEM with Low Glucose without L-Glutamine 500ml

RM345.00
Brand:

Lonza

Product Overview

DMEM is a modification of Basal Medium Eagle (BME) that contains four-times the concentrations of the amino acids and vitamins. The formulation first contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. DMEM media is suitable for most types of cells, including human, monkey, hamster, rat, mouse, chicken and fish cells.The low glucose formula is used for adherent dependent cells.

DMEM will work with different cell lines, it can also be used with primary cells such as fibroblasts.

Shipping: Ambient

Storage: 2-8° Celsius

Shelf life: 2 years from date of manufacture

All BioWhittakerTM Cell Culture Media products are for Research Use Only (RUO) and are not approved for human or veterinary use or for use in clinical or in vitro diagnostic procedures. If you require GMP grade media, contact us for more details.

DMEM with Low Glucose without L-Glutamine Brochure

Content

  • 500ml bottle

FlashGel™ DNA System

(1 customer review)
RM0.00
Brand:

Lonza

Description
5 minute DNA analysis

The FlashGel™ System is the fastest way to separate DNA and the only way to watch DNA migration as it happens. This revolutionary new tool separates DNA in 2 – 7 minutes. Monitor gel runs right at the bench, without UV light. The highly sensitive system allows a 5-20X reduction in DNA levels – saving both time and money.

  • Get results in 5 minutes or less.
  • Watch DNA migrate at your bench, in real-time without UV light.
  • Enjoy outstanding resolution and exquisite sensitivity.

The FlashGel™ System consists of enclosed, disposable, precast agarose gel cassettes and a combination electrophoresis and transilluminator unit.

  • FlashGel™ Cassettes contain precast, prestained agarose gels and buffer – no need for gel preparation, buffer addition or gel staining.
  • The FlashGel™ Dock is an electrophoresis apparatus with a built-in transilluminator that provides both separation and detection.

FlashGel™ DNA SystemBrochure

Please contact us for pricing and availability.

GelStarTM Nucleic Acid Gel Stain, 10,000X (2 x 250 uL)

RM1,293.00
Brand:

Lonza

GelStarTM Nucleic Acid Gel Stain is a highly sensitive fluorescent stain for detecting both DNA and RNA. You can choose to either add GelStarTM Stain to your agarose solution prior to casting or to post stain your gels. GelStarTM Nucleic Acid Gel Stain exhibits exceptional signal-to-noise ratio with minimal background. Maximum sensitivity, detect as little as 20 pg of dsDNA or 3 ng of RNA.

Benefits

  • Maximum sensitivity – detect as little as 20 pg of dsDNA or 3 ng of RNA,
  • Versatile – use for agarose or polyacrylamide gel electrophoresis,
  • Ideal alternative to silver staining

Applications

  • Nucleic Acid Gel Staining

MEM Eagle EBSS with L-Glutamine 1L

RM367.00
Brand:

Lonza

Product Overview

MEM eagle (EMEM) was developed in 1959 for cultivation of HeLa and L cells.

Amino acid concentrations conform closely to the protein composition of human cells. Higher concentrations of nutrients permit longer periods between feedings.

EMEM has vitamin concentrations 2-5X greater than BME and higher amino acid concentrations than BME. EMEM is suitable for culturing a broad spectrum of mammalian cells.

Shipping: Ambient

Storage: 2-8° Celsius

All BioWhittakerTM Cell Culture Media products are for Research Use Only (RUO) and are not approved for human or veterinary use or for use in clinical or in vitro diagnostic procedures. If you require GMP grade media, contact us for more details.

MEM Eagle EBSS with L-Glutamine Brochure

Content

  • 1L bottle with L-glutamine and with EBSS

MEM Eagle EBSS with L-Glutamine 500ml

RM327.00
Brand:

Lonza

Product Overview
MEM eagle (EMEM) was developed in 1959 for cultivation of HeLa and L cells.

Amino acid concentrations conform closely to the protein composition of human cells.

Higher concentrations of nutrients permit longer periods between feedings. EMEM has vitamin concentrations 2-5X greater than BME and higher amino acid concentrations than BME. EMEM is suitable for culturing a broad spectrum of mammalian cells.

Shipping: Ambient

Storage: 2-8° Celsius

Shelf life: 2 years from date of manufacture

All BioWhittakerTM Cell Culture Media products are for Research Use Only (RUO) and are not approved for human or veterinary use or for use in clinical or in vitro diagnostic procedures. If you require GMP grade media, contact us for more details.

MEM Eagle EBSS with L-Glutamine Brochure

Content

  • 500ml bottle

MEM Eagle EBSS without L-Glutamine 1L

RM380.00
Brand:

Lonza

Product Overview

MEM eagle (EMEM) was developed in 1959 for cultivation of HeLa and L cells.

Amino acid concentrations conform closely to the protein composition of human cells. Higher concentrations of nutrients permit longer periods between feedings. EMEM has vitamin concentrations 2-5X greater than BME and higher amino acid concentrations than BME. EMEM is suitable for culturing a broad spectrum of mammalian cells.

Shipping: Ambient

Storage: 15-30° Celsius

Shelf life: 2 years from date of manufacture

All BioWhittakerTM Cell Culture Media products are for Research Use Only (RUO) and are not approved for human or veterinary use or for use in clinical or in vitro diagnostic procedures. If you require GMP grade media, contact us for more details.

MEM Eagle EBSS without L-Glutamine Brochure

Content

  • 1L bottle

MEM Eagle EBSS without L-Glutamine 500ml

RM327.00
Brand:

Lonza

Product Overview

MEM eagle (EMEM) was developed in 1959 for cultivation of HeLa and L cells.

Amino acid concentrations conform closely to the protein composition of human cells. Higher concentrations of nutrients permit longer periods between feedings. EMEM has vitamin concentrations 2-5X greater than BME and higher amino acid concentrations than BME. EMEM is suitable for culturing a broad spectrum of mammalian cells.

Shipping: Ambient

Storage: 15-30° Celsius

Shelf life: 2 years from date of manufacture

All BioWhittakerTM Cell Culture Media products are for Research Use Only (RUO) and are not approved for human or veterinary use or for use in clinical or in vitro diagnostic procedures. If you require GMP grade media, contact us for more details.

MEM Eagle EBSS without L-Glutamine Brochure

Content

  • 500ml bottle

MetaPhorTM Agarose, Lonza

RM1,196.00RM16,169.00
Brand:

Lonza

MetaPhorTM Agarose, Lonza is a high resolution agarose that challenges polyacrylamide. MetaPhorTM Agarose is an intermediate melting temperature (75°C) agarose with twice the resolution capabilities of the finest-sieving agarose products. For example, a 200 bp DNA fragment can be separated from a 204 bp fragment. Using fast running protocols DNA differing in size by 1% can be resolved in as little as 1.5 hours in a 20 cm long horizontal or vertical gel format. MetaPhorTM Agarose gels (2% to 4%) approximate the resolution of polyacrylamide gels (4% to 8%). These agarose gels are ideal for resolving AMPFLPs, STRs, and tri- and tetranucleotide repeats. Ideal for small PCR fragments, MetaPhorTM Agarose is the choice agarose for resolving hard to see bands.

Benefits

  • All Lonza Agarose is quality tested to certify performance
  • Very fine resolution
  • High gel strength
  • Low EEO

Applications

  • Small Fragments
  • PCR

OEM SeaKem® LE Agarose

RM999.00
Brand:

Lonza

Buy OEM SeaKem® LE Agarose at RM999 ONLY! (Offer valid while stocks last)

Product Overview
SeaKem® LE Agarose was the first and is still the world’s most trusted agarose for nucleic acid electrophoresis. Ideal for routine analysis of nucleic acids by gel electrophoresis and blotting, each SeaKem® LE Gel sharply resolves DNA and provides consistent resolution from lot-to-lot. This molecular biology grade agarose has no detectable DNase or RNase activity and forms strong gels with low background upon ethidium bromide, SYBR® Green, or GelStarTM Staining. Due to its low EEO, DNA will have a high electrophoretic mobility. SeaKem® LE Agarose is also recommended for protein gel electrophoresis in applications such as Ouchterlony and radial immunodiffusion (RID). Information about bulk and custom packaging is available through Lonza’s Customer Service Team.

Benefits

  • All Lonza Agarose is quality tested to certify performance
  • High gel strength
  • Low EEO
  • Standard melting temperature

Applications

  • Standard DNA/RNA Electrophoreisis

Content & Storage

  • SeaKem® LE Agarose 500 g

RPMI 1640 with L-Glutamine 500ml

RM322.00
Brand:

Lonza

Product Overview

RPMI 1640 Medium was developed for culturing normal human leukocytes. It can be used for suspension and monolayer mammalian cultures.

RPMI 1640 Medium will work with different cell lines, it can also be used with primary cells such as blood/hematopoietc cells.

Shipping: Ambient

Storage: 2-8 degrees Celsius

Shelf life: 2 years from date of manufacture

All BioWhittakerTM Cell Culture Media products are for Research Use Only (RUO) and are not approved for human or veterinary use or for use in clinical or in vitro diagnostic procedures. If you require GMP grade media, contact us for more details.

Content

  • 500ml bottle
  • with L-Glutamine

SeaKem® LE Agarose

RM554.00RM6,301.00
Brand:

Lonza

SeaKem® LE Agarose was the first and is still the world’s most trusted agarose for nucleic acid electrophoresis. Ideal for routine analysis of nucleic acids by gel electrophoresis and blotting, each SeaKem® LE Gel sharply resolves DNA and provides consistent resolution from lot-to-lot. This molecular biology grade agarose has no detectable DNase or RNase activity and forms strong gels with low background upon ethidium bromide, SYBR® Green, or GelStarTM Staining. Due to its low EEO, DNA will have a high electrophoretic mobility. SeaKem® LE Agarose is also recommended for protein gel electrophoresis in applications such as Ouchterlony and radial immunodiffusion (RID).

Benefits

  • All Lonza Agarose is quality tested to certify performance
  • High gel strength
  • Low EEO
  • Standard melting temperature

Applications

  • Standard DNA/RNA Electrophoreisis

TheraPEAK™ MSCGM™ CD Serum-free Mesenchymal Stem Cell Growth Medium BulletKit™

RM678.00
Brand:

Lonza

Description

Stem cells possess the unique ability to proliferate as an undifferentiated cell type, then, when properly stimulated, differentiate into multiple lineages of cells. Lonza’s TheraPEAK™ MSCGM-CD™ Chemically Defined Mesenchymal Stem Cell Medium is a chemically defined, serum-free, xeno-free media designed to proliferate human bone marrow derived mesenchymal cells in an undifferentiated state. The medium has been specifically designed and tested to ensure that the medium neither promotes spontaneous differentiation of cells nor inhibits cell differentiation to osteogenic, chondrogenic, or adipogenic lineages (when properly stimulated with an appropriate differentiation media). Serum-free media, such as TheraPEAK™ MSCGM-CD™ Chemically Defined Mesenchymal Stem Cell Medium, are intended to support cell growth without serum supplementation. This medium is chemically defined indicating that it contain only constituents of known molecular structure. Lonza's TheraPEAK™ MSCGM-CD™ Chemically Defined Mesenchymal Stem Cell Medium is of primary interest to those seeking maximal control over research and manufacturing variables. TheraPEAK™ MSCGM-CD™ Chemically Defined Mesenchymal Stem Cell Medium's formulation is complete and contains human albumin, recombinant human insulin, pasteurized human transferrin, HEPES, and L-glutamine. TheraPEAK™ MSCGM-CD™ Chemically Defined Mesenchymal Stem Cell Medium does not contain phenol red or antibiotics. The basal medium (MSCBM-CD Mesenchymal Basal Medium, Chemically defined; catalog no.00190620) and the necessary supplements (MSCGM-CD™ SingleQuotsTMKit ; catalog no. 00192125) are included in this BulletKit.

TheraPEAK™ MSCGM™ CD Serum-free Mesenchymal Stem Cell Growth Medium BulletKit™ Brochure

X-VIVO™ 10 Serum-free Hematopoietic Cell Medium, xenofree

RM678.00RM719.00
Brand:

Lonza

Description

The X-VIVO™ 10 Media formulations are designed to support the generation of LAK cells in a serum-free environment. The original protocols involved the incubation of patient or normal donor peripheral blood lymphocytes (PBL) at 1.0 – 3.0 × 106 cells/ml for a period of 3 days in the presence of 1,000 Cetus units of rIL-2/ml. Optimal LAK cell generation is achieved when peripheral blood lymphocytes are incubated for 3 – 10 days at a density of 1.0 – 6.0 × 106 cells/ml in the presence of 100 – 1,000 Cetus units of rIL-2. X-VIVO™ 10 Media is available as a 1X liquid in two convenient formulations.

Key Applications:
-Proliferation of peripheral blood lymphocytes
-Proliferation of tumor infiltrating lymphocytes
-Cryopreservation of human tissue
-Cultivation of human monocytes and macrophages
-Cultivation of stem cells
-Cultivation of dendritic cells

X-VIVO™ 15, Serum free hematopoietic cell medium, xenofree

RM740.00RM798.00
Brand:

Merck

Description

X-VIVO™ 15 Media are similar in composition to X-VIVO™ 10 Media and have been optimized for the proliferation of tumor infiltrating lymphocytes (TIL) under serum-free conditions. X-VIVO™ 15 Media support the proliferation of purified CD3+ cells isolated from peripheral blood and human tumors. X-VIVO™ 15 Media can also be used to support the growth of human monocytes, macrophage cells and cell lines, PBL, granulocytes, and natural killer (NK) cells. In addition, X-VIVO™ 15 Media provide a serum-free environment for the expansion of HUT-78 and related human lymphocytic cell lines.

Applications:
-Proliferation of peripheral blood lymphocytes
-Proliferation of tumor infiltrating lymphocytes
-Cryopreservation of human tissue
-Cultivation of human monocytes and macrophages
-Cultivation of stem cells
-Cultivation of dendritic cells
-Cultiviation of CAR-T cells

X-VIVO™ 20 Serum-free Hematopoietic Cell Medium

RM678.00
Brand:

Lonza

Description

X-VIVO™ 20 Medium is optimized to support the generation of lymphokine activated killer (LAK) cells from monocytedepleted peripheral blood lymphocytes (PBL) at high density. Initial cell densities between 2.0 – 3.0 × 107 cells/ ml can be used to successfully generate LAK cells. X-VIVO™ 20 Medium may also be used as a growth medium for PBL and tumor infiltrating lymphocytes (TIL).

All BioWhittaker™ Cell Culture Media products are for Research Use Only (RUO) and are not approved for human or veterinary use or for use in clinical or in vitro diagnostic procedures.