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dATP Solution – 25 umol

Brand:

New England Biolabs

Enhance your PCR Reaction with our ultrapure dATP solution

  • Compatible with all NEB DNA polymerases in a variety of applications
  • ≥ 99% purity as determined by HPLC analysis
  • Also available in solution mix (NEB #N0447) or set (NEB #N0446) format

Immobilized T4 DNA Ligase

Brand:

New England Biolabs

Characteristics of Immobilized T4 DNA Ligase:

  • Immobilized T4 DNA Ligase (IM T4 DNA Ligase) consists of T4 DNA Ligase covalently bound to magnetic beads.
  • Easily remove enzyme from reaction using a magnet
  • Avoid heat inactivation step, which may interfere with downstream applications
  • Re-use immobilized enzymes
  • Enable new workflows and applications

Instant Sticky-end Ligase Master Mix

Brand:

New England Biolabs

Specifically formulated to rapidly ligate cohesive-end (2–4 bp) substrates and improve transformation, Instant Sticky-end Ligase Master Mix is a ready-to-use 2X solution of T4 DNA Ligase and a proprietary ligation enhancer in an optimized reaction buffer.

  • No incubation time necessary to achieve ligation
  • Efficiencies sufficient for successful sub-cloning of sticky-end substrates
  • No thawing of the master mix is required as liquid state is maintained when stored at -20°C
  • Simplified reaction setup with optimized ratio of enzyme and buffer components
  • Ligations for subcloning can be carried out in small volumes with low DNA concentrations, allowing users to conserve precious DNA samples and directly transform many strains of chemically competent E. coli without dilution
  • Not sure which ligase to choose? Refer to our DNA and RNA Ligase Properties Chart

Salt-T4® DNA Ligase

Brand:

New England Biolabs

Characteristics of Salt-T4 DNA Ligase: