Bst DNA Polymerase Lg Frag
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New England Biolabs
The original polymerase for Loop-Mediated Isothermal Amplification (LAMP)
- Modified to retain 5´ → 3´ polymerase activity while lacking 5´ →3´ exonuclease activity
- Suitable for applications requiring thermophilic strand displacement
- Supplied with ThermoPol® Reaction Buffer for high product yields in demanding conditions
dATP Solution – 25 umol
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New England Biolabs
Enhance your PCR Reaction with our ultrapure dATP solution
- Compatible with all NEB DNA polymerases in a variety of applications
- ≥ 99% purity as determined by HPLC analysis
- Also available in solution mix (NEB #N0447) or set (NEB #N0446) format
Immobilized T4 DNA Ligase
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New England Biolabs
Characteristics of Immobilized T4 DNA Ligase:
- Immobilized T4 DNA Ligase (IM T4 DNA Ligase) consists of T4 DNA Ligase covalently bound to magnetic beads.
- Easily remove enzyme from reaction using a magnet
- Avoid heat inactivation step, which may interfere with downstream applications
- Re-use immobilized enzymes
- Enable new workflows and applications
Instant Sticky-end Ligase Master Mix
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New England Biolabs
Specifically formulated to rapidly ligate cohesive-end (2–4 bp) substrates and improve transformation, Instant Sticky-end Ligase Master Mix is a ready-to-use 2X solution of T4 DNA Ligase and a proprietary ligation enhancer in an optimized reaction buffer.
- No incubation time necessary to achieve ligation
- Efficiencies sufficient for successful sub-cloning of sticky-end substrates
- No thawing of the master mix is required as liquid state is maintained when stored at -20°C
- Simplified reaction setup with optimized ratio of enzyme and buffer components
- Ligations for subcloning can be carried out in small volumes with low DNA concentrations, allowing users to conserve precious DNA samples and directly transform many strains of chemically competent E. coli without dilution
- Not sure which ligase to choose? Refer to our DNA and RNA Ligase Properties Chart
LunaScript®RT SuperMix Kit
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New England Biolabs
The LunaScript® RT SuperMix Kit is optimized for cDNA synthesis in a two-step RT-qPCR workflow.
Make a simpler choice
- Single-tube SuperMix contains random hexamer and oligo-dT primers, dNTPs, Murine RNase Inhibitor, and Luna® Reverse Transcriptase
- Non-interfering, visible tracking dye helps to eliminate pipetting errors
- Combine with Luna qPCR master mixes for robust RT-qPCR results
Experience best-in-class performance
Illuminate your two-step RT-qPCR
Q5® High-Fidelity DNA Polymerase, NEB
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New England Biolabs
Q5® High-Fidelity DNA Polymerase, NEB sets a new standard for both fidelity and robust performance. With the highest-fidelity amplification available (~280 times higher than Taq), Q5 DNA Polymerase results in ultra-low error rates. Q5 DNA Polymerase is composed of a novel polymerase that is fused to the processivity-enhancing Sso7d DNA binding domain, improving speed, fidelity and reliability of performance.
Features of Q5® High-Fidelity DNA Polymerase, NEB:
- Highest fidelity amplification (~280X higher than Taq)
- Ultra-low error rates
- Superior performance for a broad range of amplicons (from high AT to high GC)
- Hot start and master mix formats available
Salt-T4® DNA Ligase
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New England Biolabs
Characteristics of Salt-T4 DNA Ligase:
- Salt-T4 DNA Ligase is an engineered variant of T4 DNA Ligase with improved salt tolerance
- Capable of joining blunt end and cohesive end termini, as well as repairing single stranded nicks in duplex DNA and some DNA/RNA hybrids.
- Supplied with both T4 DNA Ligase Buffer and StickTogether™ DNA Ligase Buffer.
- Other T4 DNA Ligase products include Quick Ligation Kit, Hi-T4, Instant Sticky-end Ligase Master Mix and Blunt/TA Ligase Master Mix
- Not sure which ligase to choose? Refer to our DNA and RNA Ligase Properties Chart or DNA Ligase Selection Chart
