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The original polymerase for Loop-Mediated Isothermal Amplification (LAMP)

• Modified to retain 5´ → 3´ polymerase activity while lacking 5´ →3´ exonuclease activity
• Suitable for applications requiring thermophilic strand displacement
• Supplied with ThermoPol^® Reaction Buffer for high product yields in demanding conditions

The LunaScript^® RT SuperMix Kit is optimized for cDNA synthesis in a two-step RT-qPCR workflow.

Make a simpler choice

• Single-tube SuperMix contains random hexamer and oligo-dT primers, dNTPs, Murine RNase Inhibitor, and Luna^® Reverse Transcriptase
• Non-interfering, visible tracking dye helps to eliminate pipetting errors
• Combine with Luna qPCR master mixes for robust RT-qPCR results

Experience best-in-class performance

• Less than 15 minute first-strand cDNA synthesis protocol
• All Luna products have undergone rigorous testing to optimize specificity, sensitivity, accuracy and reproducibility
• A comprehensive evaluation of commercially-available qPCR and RT-qPCR reagents demonstrates superior performance of Luna products

Illuminate your two-step RT-qPCR

• Novel, thermostable Luna Reverse Transcriptase improves performance
• Consistent linearity, sensitivity, and capacity for reliable RNA quantification

Q5^® High-Fidelity DNA Polymerase, NEB sets a new standard for both fidelity and robust performance. With the highest-fidelity amplification available (~280 times higher than Taq), Q5 DNA Polymerase results in ultra-low error rates. Q5 DNA Polymerase is composed of a novel polymerase that is fused to the processivity-enhancing Sso7d DNA binding domain, improving speed, fidelity and reliability of performance.

Features of Q5^® High-Fidelity DNA Polymerase, NEB:

• Highest fidelity amplification (~280X higher than Taq)
• Ultra-low error rates
• Superior performance for a broad range of amplicons (from high AT to high GC)
• Hot start and master mix formats available