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Gellan Gum, Plant Cell Technology

Brand:

Plant Cell Technology

Gellan gum also known as Phytagel, Gelzan, Gelrite, or Applied gel is a water soluble anionic polysaccharide produced by fermentation of a carbohydrate by pseudomonas Elodea, purified, dried, and powdered.
Gellan gum is ideal for preparation of nutrient media for in plant tissue culture work and is a substitute for agar.Gels produced by gellan gum are clearer in comparison to agar however the gel is more rigid and brittle than agar gel. Gellan gum requires roughly half the amount of agar that is needed. Gellan gum melts at 80°C– 100°C and gels between 10°C-50°C.Gellan gum also known as Phytagel, Gelzan, Gelrite, or Applied gel is a water soluble anionic polysaccharide produced by fermentation of a carbohydrate by pseudomonas Elodea, purified, dried, and powdered.
Gellan gum is ideal for preparation of nutrient media for in plant tissue culture work and is a substitute for agar.Gels produced by gellan gum are clearer in comparison to agar however the gel is more rigid and brittle than agar gel. Gellan gum requires roughly half the amount of agar that is needed. Gellan gum melts at 80°C– 100°C and gels between 10°C-50°C.

Gel Strength: >900 GM/CM2

Application:

Gellan Gum is used as microbial solid culturing media. The substance is often used in doses ranging between 0.2% to 0.4% (w/v) to solidify plant tissue culture media. PPM can be mixed in when creating Gellan Gum media

Plant Preservative Mixture (PPM), Plant Cell Technology

Brand:

Plant Cell Technology

Plant Preservative Mixture (PPM), is a broad-spectrum biocide/ for plant tissue culture. PPM™ is the ultimate solution to the never-ending struggle against microbial airborne, waterborne and endogenous contamination.

PPM™ is a heat-stable preservative/biocide which can be used to effectively prevent or reduces microbial contamination in plant tissue culture.

At optimum doses, PPM™, which stands for Plant Preservative Mixture™, is an extremely effective Preservative/Biocide, yet does not impair in vitro seed germination, callus proliferation and callus regeneration.

PPM™ prevents the germination of both bacteria and fungi spores. It is heat stable and therefore can be autoclaved with the media.
PPM™ can be, and should be used as a standard ingredient in plant tissue culture media, and is also substantially less expensive than commonly used antibiotics.

While PPM™ was principally designed to inhibit airborne contamination, waterborne contamination and contamination introduced from human contact, it can also — in many cases — be used to reduce endogenous contamination.

Despite the most stringent use of sterile techniques, the contamination of plant cell and plant tissue cultures remains a persistent problem that can result in losses ranging from small number of cultures to the loss of whole batches.

Supreme TC Grade Agar, Plant Cell Technology

Brand:

Plant Cell Technology

A polysaccharide complex obtained through bleaching and hot water extraction of agarocytes from the red alga Rhodophyceae. Typical usage rate of 6 – 12 g/L medium. Supreme agar offers greater clarity of plant culture media.
Gel Strength: 1050 GM/CM2

Application:

Agar is used as microbial solid culturing media. The substance is often used in doses ranging between 0.5 to 1.0% (w/v) to solidify plant tissue culture media. Agar is an essential ingredient used for in vitro root development.