Brand:
Thermo ScientificTM OxoidTM
- Oxoid STAA Agar Base is used for the isolation of Brochothrix thermosphactafrom food samples.
- Also available STAA Selective Supplement, Part No. SR0151E and STA Selective Supplement, Part No. SR0162E.
STAA Agar Base, Oxoid Composition
| Typical Formula* |
gm/litre
|
| Peptone |
20.0
|
| Yeast extract |
2.0
|
| Dipotassium hydrogen phosphate |
1.0
|
| Magnesium sulphate |
1.0
|
| Agar |
13.0
|
| pH 7.0 ± 0.2 @ 25°C |
|
STAA Agar, Oxoid Preparation:
Suspend 18.5g of STAA Agar Base in 500ml distilled water and bring gently to the boil to dissolve completely. Add 7.5g of glycerol and sterilise by autoclaving at 121° C for 15 minutes. Cool to 50°C and aseptically add:
a) for STAA Medium the contents of 1 vial of STAA Selective Supplement SR0151 reconstituted as directed.
b) for STA Medium the contents of 1 vial of STA Selective Supplement SR0162 reconstituted as directed.
Mix well and distribute into sterile Petri dishes.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
The prepared medium may be stored for up to 2 weeks at 2-8°C.