Question 1

What are restriction enzymes?

Accepted Answer

Restriction Enzymes (aka 'molecular scissors') are enzymes produced by bacteria to cut the specific site of DNA, which also known as the recognition site or restriction site. Each restriction enzyme can recognize these unique recognition sites of DNA and cut the DNA into fragments.

Question 2

What are blunt ends?

Accepted Answer

Blunt ends refer to the base pair on both strands of a DNA end at the same base pair. We also name blunt ends as non-cohesive ends, since there is no unpaired DNA strand fleeting at the end of DNA.

Question 3

What are sticky ends?

Accepted Answer

Sticky ends are known as cohesive/non-blunt ends. The REs (EcoRI-hf and BamHI-hf) cut the DNA strands a few base pair from each other. This cutting results in the formation of 5'- or 3'- of unpaired DNA strands. We name them as 5'- (5 prime) or 3' (3 prime) overhang.

Question 4

What are the example of blunt ends restriction enzymes?

Accepted Answer

Example of the REs that create the blunt ends are AfeI and AluI.

Restriction Enzymes

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