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FEATURED PRODUCTS

Visit our shop to see amazing products.

Breast Cancer Genetics – Electrophoresis Education kit

Brand:

Cleaver Scientific

Breastcancer is the most common type of cancer. About 5-10% of all breast cancers areinherited. Mutations in the breast cancer 1 (BRCA1) gene greatly increase therisk of developing breast and ovarian cancer. This gene is found on chromosome17 and is normally responsible for DNA repair. It is therefore called a tumoursuppressor gene. Mutations in BRCA1 are dominant and so the presence of onecopy of a mutated form can cause an increased risk of cancer.

This experiment will test DNA of members of a family where the grandmother (generation 2) and her daughter(generation 3) both died of breast cancer. The deceased women had a mutation intheir BRCA1 gene.

Each kit included enough DNA, agarose gel, electrophoesis buffer and DNA stain for up to 8 groups of students (8 gels).

Key Features

  • Whole experiment takes less that 1 hour.
  • Great for introducing genetic diseases and genetic screening to students
  • Works perfectly with Cleaver Scientific electrophoresis equipment

FOX10 Cefoxitin

Brand:

Thermo ScientificTM OxoidTM

** This item is not available at the moment. Please contact us for more information.

Manually determine the antibiotic susceptibility of microorganisms using Antimicrobial Susceptibility Testing (AST) methods in conjunction with reliable, easy-to-use Thermo Scientific™ Oxoid™ Cefoxitin Antimicrobial Susceptibility Disks.

Disk Dispenser 8 Place 90mm Skirt

Brand:

Thermo ScientificTM OxoidTM

Accurately place antimicrobial susceptibility disks onto your media of choice when carrying out manual Antimicrobial Susceptibility Testing methods using a self-tamping Thermo Scientific™ Oxoid™ Antimicrobial Susceptibility Disk Dispenser.

Brom Thymol Blue (1%) BTL/25ml

Brand:

Thermo Scientific™ Remel™

Thermo Scientific™ Remel Brom Thymol Blue is a pH indicator for use with microbiological culture media and for confirmation of negative reactions in C-H-O Carbohydrates.

McFarland Turb Standard 5.0

Brand:

Thermo Scientific™ Remel™

  • Thermo Scientific™ Remel McFarland Equivalence Turbidity Standard is packaged in plastic case with a visual comparison card.
  • Tube size is 15 x 103mm and fits in most spectrophotometers.
  • Polystyrene technology allows longer shelf life.

Salmonella Selective Supplement

Brand:

Thermo ScientificTM OxoidTM

Oxoid Salmonella Selective Supplement is used for the presumptive identification of Salmonella species from clinical and food samples.

  • Add to Chromogenic Salmonella Agar Base, Part No. CM1007B or Brilliance™ Salmonella Agar Base, Part No. CM1092B
  • Each vial supplements 500mL of medium

Salmonella Selective Supplement, Oxoid Composition

Vial Contents (each vial is sufficient for 500ml of medium)per vialper litre
Novobiocin

2.5mg

5mg

Cefsulodin

6mg

12mg

 

Chromogenic Salmonella Agar, Oxoid Preparation:
Suspend 25g in 500ml of distilled water and add the contents of one vial of Salmonella Selective Supplement (SR0194) reconstituted as directed. Mix well and bring to the boil with frequent agitation. DO NOT AUTOCLAVE. DO NOT HOLD AT BOILING TEMPERATURE. Cool to 50°C, mix well and pour into sterile Petri dishes.

Brilliance Salmonella Agar, Oxoid Preparation:
Suspend 27g of Brilliance Salmonella Agar Base in 500ml of distilled water. Add the contents of 1 vial of Salmonella Selective Supplement, resuspended as directed. Mix well and sterilise by bringing to the boil with frequent agitation. Cool to around 50ºC, mix well and pour into sterile Petri dishes.
NOTE: It is critical that the selective supplement is added prior to heating. When preparing volumes greater than 1 litre contact Oxoid Technical Support for directions.

Storage conditions and Shelf life
Salmonella Selective Supplement should be stored in the dark at 2-8ºC. When stored as directed, the un-opened products will remain stable until the expiry date printed on the packaging.

Dor10 Doripenem 10µg

Brand:

Thermo ScientificTM OxoidTM

Manually determine the antibiotic susceptibility of microorganisms using Antimicrobial Susceptibility Testing (AST) methods in conjunction with reliable, easy-to-use Thermo Scientific™ Oxoid™ Doripenem Antimicrobial susceptibility Disks.

Streptomycin 300 (S300)

Brand:

Thermo ScientificTM OxoidTM

Manually determine the antibiotic susceptibility of microorganisms using Antimicrobial Susceptibility Testing (AST) methods in conjunction with reliable, easy-to-use Thermo Scientific™ Oxoid™ Streptomycin Antimicrobial Susceptibility Disks.

Cary-Blair Medium 500g

Brand:

Thermo ScientificTM OxoidTM

Oxoid Cary-Blair Transport Medium is a transport medium for gram-negative and anaerobic microorganisms.

Cary-Blair Transport Medium, Oxoid Composition

Typical Formula*

gm/litre

Disodium hydrogen phosphate

1.1

Sodium thioglycollate

1.5

Sodium chloride

5.0

Calcium chloride

0.09

Agar

5.6

pH 8.4 ± 0.2 @ 25°C

 

Cary-Blair Transport Medium, Oxoid Preparation:
Suspend 13.3g in 1 litre of distilled water and bring gently to the boil to dissolve the agar. Distribute into small, screw-cap bottles and sterilise by immersing in free-steam for 15 minutes. Allow to cool and tighten the screw caps to prevent water loss.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
The prepared medium should be stored away from light at 2-8°C or at room temperature (22-25°C) up to 19 months.

Precautions
The medium should not be incubated to check sterility, prior to use. This should be carried out on separate quality control samples.
The medium can maintain the viability of fastidious organisms for transport purposes but it should not be used as a storage or enrichment medium.
The results obtained from the medium are dependent on the quality of the specimen material. Commensal anaerobic organisms may overgrow in the medium and cause misleading results.

Complete 2-D Systems – Mini to Maxi

Brand:

Cleaver Scientific

The omniPAGE 2-D Systems include both modules required for Slab Gel and First Dimension Electrophoresis and accessories, to provide a complete Mini, Mini Wide or Maxi 2-D system. The Tube Gel Module includes a rapid release gasket for easy tube extraction. Focusing can be accomplished in as little as three hours in the Mini Unit. Disposable capillary tubes are included for added convenience, plus 2-D combs and spacers which are colour coded according to thickness for easy identification.

Package includes:

  • 1x omniPAGE Mini Vertical Unit which includes: 2x2mm thick notched glass plates, 2x2mm thick plain glass plates with 1mm thick bonded spacers, 1x dummy plate, 2x combs (1mm thick 12 samples), 1x casting base, silicone mat, cooling pack
  • 1x Capillary electrophoresis module which includes: internal running module for tube gels, capillary tubes, blanking plugs, 2-D combs and spacers

Anaerojar Base, Oxoid

Brand:

Thermo ScientificTM OxoidTM

Oxoid AnaeroJar Base is a polycarbonate jar base for use with AnaeroJar 2.5L, Part No. AG0025A.

Trypsin from Porcine Pancreas (Sigma)

Brand:

Sigma

Description

Product line: BioReagent

Biological source: Porcine pancreas

Specific activity: 1,000-2,000 BAEE units/mg solid

Trypsin is applicable for tissue disaggregation, due to its effective action and tolerance towards different cell types and serum-induced neutralization.[1]

For trypsin digestion of peptides, use a ratio of about 1:100 to 1:20 for trypsin:peptide. The typical use for this product is in removing adherent cells from a culture surface. The concentration of trypsin necessary to dislodge cells from their substrate is dependent primarily on the cell type and the age of the culture. Trypsins have also been used for the re-suspension of cells during cell culture, in proteomics research for digestion of proteins and in various in-gel digestionsns†. Additional applications include assessing crystallization by membrane-based techniques and in a study to determine that protein folding rates and yields can be limited by the presence of kinetic traps.
Trypsin from porcine pancreas has been used to digest chicken bones.[2] It has also been used in the isolation of luteal endothelial cells.[3][4][5]

OmniPur Water, WFI Quality, Sterile Purified Water, Cell Culture Tested – CAS7732-18-5, 1L

Brand:

Merck

** This product has been discontinued.

OmniPur Water is sterile water that is tested to be DNase free, Protease free, RNase free and bacterial free.

It is suitable to be used in:

  • Cell Biology
    • Cell culture media preparation, extraction buffer, Molecular work, reconstitute of compound
    • Diluent for live cells staining
  • Sterile solution preparation Microbiology Sample Preparation
    • Diluent and rinsing reagent in Microbial Membrane Filtration

Key Specification:

  1. Sterile by USP Standard
  2. DNase, RNase, Protease-Free
  3. < 500 ppb Total Organic Carbon
  4. < 0.025 EU/ml Bacterial Endotoxin

COA for Omnipur Sterile Water

Minimum Essential Medium Eagle (EMEM) with Alpha Modification, powder form

Brand:

Sigma-Aldrich

This is the most enriched variation of the EMEM formulation offered that suitable for cell culuture. It contains all 21 normal amino acids, some at increased concentrations. In addition, it contains 5 additional vitamins. Refer to the table below and choose your preferecd EMEM!

Minimum Essential Medium Eagle (EMEM) with Alpha Modification, powder form Brochure

OEM SeaKem® LE Agarose

Brand:

Lonza

Product Overview
SeaKem® LE Agarose was the first and is still the world’s most trusted agarose for nucleic acid electrophoresis. Ideal for routine analysis of nucleic acids by gel electrophoresis and blotting, each SeaKem® LE Gel sharply resolves DNA and provides consistent resolution from lot-to-lot. This molecular biology grade agarose has no detectable DNase or RNase activity and forms strong gels with low background upon ethidium bromide, SYBR® Green, or GelStarTM Staining. Due to its low EEO, DNA will have a high electrophoretic mobility. SeaKem® LE Agarose is also recommended for protein gel electrophoresis in applications such as Ouchterlony and radial immunodiffusion (RID). Information about bulk and custom packaging is available through Lonza’s Customer Service Team.

Benefits

  • All Lonza Agarose is quality tested to certify performance
  • High gel strength
  • Low EEO
  • Standard melting temperature

Applications

  • Standard DNA/RNA Electrophoreisis

Content & Storage

  • SeaKem® LE Agarose 500 g

1 kb DNA Ladder, NEB

Brand:

New England Biolabs

This 1 kb DNA ladder is available in Quick-Load Purple format – enjoy the convenience of a ready-to-load format.

1 kb DNA ladder from New England Biolabs (NEB) is suitable for use with molecular weight standard for agarose gel electrophoresis. A number of proprietary plasmids are digested to completion with appropriate restriction enzymes to yield 10 bands, ranging from 0.5 to 10 kilobases. NEB set 3.0 kb fragment as a reference band by increasing the intensity of that band.

  • Size range: 500 bp to 10 kb
  • Value pricing
  • Supplied with a free vial of Gel Loading Dye, Purple (6X), no SDS (NEB #B7025).
  • Easy to identify reference bands
  • Small size suitable for 200 gel lanes; large size suitable for 1000 gel lanes

Q5 Hot Start High-Fidelity 2X Master Mix

Brand:

New England Biolabs

Fidelity at its finest

Q5® High-Fidelity DNA Polymerase sets a new standard for both fidelity and robust performance. With the highest fidelity amplification available (~280 times higher than Taq), Q5 DNA Polymerase results in ultra-low error rates. Q5 DNA Polymerase is composed of a novel polymerase that is fused to the processivity-enhancing Sso7d DNA binding domain, improving speed, fidelity and reliability of performance.

  • Highest fidelity amplification (~280X higher than Taq)
  • Ultra-low error rates
  • Superior performance for a broad range of amplicons (from high AT to high GC)
  • Hot start and master mix formats available

FEATURED PRODUCTS

Visit our shop to see amazing products.

Breast Cancer Genetics – Electrophoresis Education kit

Brand:

Cleaver Scientific

Breastcancer is the most common type of cancer. About 5-10% of all breast cancers areinherited. Mutations in the breast cancer 1 (BRCA1) gene greatly increase therisk of developing breast and ovarian cancer. This gene is found on chromosome17 and is normally responsible for DNA repair. It is therefore called a tumoursuppressor gene. Mutations in BRCA1 are dominant and so the presence of onecopy of a mutated form can cause an increased risk of cancer.

This experiment will test DNA of members of a family where the grandmother (generation 2) and her daughter(generation 3) both died of breast cancer. The deceased women had a mutation intheir BRCA1 gene.

Each kit included enough DNA, agarose gel, electrophoesis buffer and DNA stain for up to 8 groups of students (8 gels).

Key Features

  • Whole experiment takes less that 1 hour.
  • Great for introducing genetic diseases and genetic screening to students
  • Works perfectly with Cleaver Scientific electrophoresis equipment

FOX10 Cefoxitin

Brand:

Thermo ScientificTM OxoidTM

** This item is not available at the moment. Please contact us for more information.

Manually determine the antibiotic susceptibility of microorganisms using Antimicrobial Susceptibility Testing (AST) methods in conjunction with reliable, easy-to-use Thermo Scientific™ Oxoid™ Cefoxitin Antimicrobial Susceptibility Disks.

Disk Dispenser 8 Place 90mm Skirt

Brand:

Thermo ScientificTM OxoidTM

Accurately place antimicrobial susceptibility disks onto your media of choice when carrying out manual Antimicrobial Susceptibility Testing methods using a self-tamping Thermo Scientific™ Oxoid™ Antimicrobial Susceptibility Disk Dispenser.

Brom Thymol Blue (1%) BTL/25ml

Brand:

Thermo Scientific™ Remel™

Thermo Scientific™ Remel Brom Thymol Blue is a pH indicator for use with microbiological culture media and for confirmation of negative reactions in C-H-O Carbohydrates.

McFarland Turb Standard 5.0

Brand:

Thermo Scientific™ Remel™

  • Thermo Scientific™ Remel McFarland Equivalence Turbidity Standard is packaged in plastic case with a visual comparison card.
  • Tube size is 15 x 103mm and fits in most spectrophotometers.
  • Polystyrene technology allows longer shelf life.

Salmonella Selective Supplement

Brand:

Thermo ScientificTM OxoidTM

Oxoid Salmonella Selective Supplement is used for the presumptive identification of Salmonella species from clinical and food samples.

  • Add to Chromogenic Salmonella Agar Base, Part No. CM1007B or Brilliance™ Salmonella Agar Base, Part No. CM1092B
  • Each vial supplements 500mL of medium

Salmonella Selective Supplement, Oxoid Composition

Vial Contents (each vial is sufficient for 500ml of medium)per vialper litre
Novobiocin

2.5mg

5mg

Cefsulodin

6mg

12mg

 

Chromogenic Salmonella Agar, Oxoid Preparation:
Suspend 25g in 500ml of distilled water and add the contents of one vial of Salmonella Selective Supplement (SR0194) reconstituted as directed. Mix well and bring to the boil with frequent agitation. DO NOT AUTOCLAVE. DO NOT HOLD AT BOILING TEMPERATURE. Cool to 50°C, mix well and pour into sterile Petri dishes.

Brilliance Salmonella Agar, Oxoid Preparation:
Suspend 27g of Brilliance Salmonella Agar Base in 500ml of distilled water. Add the contents of 1 vial of Salmonella Selective Supplement, resuspended as directed. Mix well and sterilise by bringing to the boil with frequent agitation. Cool to around 50ºC, mix well and pour into sterile Petri dishes.
NOTE: It is critical that the selective supplement is added prior to heating. When preparing volumes greater than 1 litre contact Oxoid Technical Support for directions.

Storage conditions and Shelf life
Salmonella Selective Supplement should be stored in the dark at 2-8ºC. When stored as directed, the un-opened products will remain stable until the expiry date printed on the packaging.

Dor10 Doripenem 10µg

Brand:

Thermo ScientificTM OxoidTM

Manually determine the antibiotic susceptibility of microorganisms using Antimicrobial Susceptibility Testing (AST) methods in conjunction with reliable, easy-to-use Thermo Scientific™ Oxoid™ Doripenem Antimicrobial susceptibility Disks.

Streptomycin 300 (S300)

Brand:

Thermo ScientificTM OxoidTM

Manually determine the antibiotic susceptibility of microorganisms using Antimicrobial Susceptibility Testing (AST) methods in conjunction with reliable, easy-to-use Thermo Scientific™ Oxoid™ Streptomycin Antimicrobial Susceptibility Disks.

Cary-Blair Medium 500g

Brand:

Thermo ScientificTM OxoidTM

Oxoid Cary-Blair Transport Medium is a transport medium for gram-negative and anaerobic microorganisms.

Cary-Blair Transport Medium, Oxoid Composition

Typical Formula*

gm/litre

Disodium hydrogen phosphate

1.1

Sodium thioglycollate

1.5

Sodium chloride

5.0

Calcium chloride

0.09

Agar

5.6

pH 8.4 ± 0.2 @ 25°C

 

Cary-Blair Transport Medium, Oxoid Preparation:
Suspend 13.3g in 1 litre of distilled water and bring gently to the boil to dissolve the agar. Distribute into small, screw-cap bottles and sterilise by immersing in free-steam for 15 minutes. Allow to cool and tighten the screw caps to prevent water loss.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
The prepared medium should be stored away from light at 2-8°C or at room temperature (22-25°C) up to 19 months.

Precautions
The medium should not be incubated to check sterility, prior to use. This should be carried out on separate quality control samples.
The medium can maintain the viability of fastidious organisms for transport purposes but it should not be used as a storage or enrichment medium.
The results obtained from the medium are dependent on the quality of the specimen material. Commensal anaerobic organisms may overgrow in the medium and cause misleading results.

Complete 2-D Systems – Mini to Maxi

Brand:

Cleaver Scientific

The omniPAGE 2-D Systems include both modules required for Slab Gel and First Dimension Electrophoresis and accessories, to provide a complete Mini, Mini Wide or Maxi 2-D system. The Tube Gel Module includes a rapid release gasket for easy tube extraction. Focusing can be accomplished in as little as three hours in the Mini Unit. Disposable capillary tubes are included for added convenience, plus 2-D combs and spacers which are colour coded according to thickness for easy identification.

Package includes:

  • 1x omniPAGE Mini Vertical Unit which includes: 2x2mm thick notched glass plates, 2x2mm thick plain glass plates with 1mm thick bonded spacers, 1x dummy plate, 2x combs (1mm thick 12 samples), 1x casting base, silicone mat, cooling pack
  • 1x Capillary electrophoresis module which includes: internal running module for tube gels, capillary tubes, blanking plugs, 2-D combs and spacers

Anaerojar Base, Oxoid

Brand:

Thermo ScientificTM OxoidTM

Oxoid AnaeroJar Base is a polycarbonate jar base for use with AnaeroJar 2.5L, Part No. AG0025A.

Trypsin from Porcine Pancreas (Sigma)

Brand:

Sigma

Description

Product line: BioReagent

Biological source: Porcine pancreas

Specific activity: 1,000-2,000 BAEE units/mg solid

Trypsin is applicable for tissue disaggregation, due to its effective action and tolerance towards different cell types and serum-induced neutralization.[1]

For trypsin digestion of peptides, use a ratio of about 1:100 to 1:20 for trypsin:peptide. The typical use for this product is in removing adherent cells from a culture surface. The concentration of trypsin necessary to dislodge cells from their substrate is dependent primarily on the cell type and the age of the culture. Trypsins have also been used for the re-suspension of cells during cell culture, in proteomics research for digestion of proteins and in various in-gel digestionsns†. Additional applications include assessing crystallization by membrane-based techniques and in a study to determine that protein folding rates and yields can be limited by the presence of kinetic traps.
Trypsin from porcine pancreas has been used to digest chicken bones.[2] It has also been used in the isolation of luteal endothelial cells.[3][4][5]

OmniPur Water, WFI Quality, Sterile Purified Water, Cell Culture Tested – CAS7732-18-5, 1L

Brand:

Merck

** This product has been discontinued.

OmniPur Water is sterile water that is tested to be DNase free, Protease free, RNase free and bacterial free.

It is suitable to be used in:

  • Cell Biology
    • Cell culture media preparation, extraction buffer, Molecular work, reconstitute of compound
    • Diluent for live cells staining
  • Sterile solution preparation Microbiology Sample Preparation
    • Diluent and rinsing reagent in Microbial Membrane Filtration

Key Specification:

  1. Sterile by USP Standard
  2. DNase, RNase, Protease-Free
  3. < 500 ppb Total Organic Carbon
  4. < 0.025 EU/ml Bacterial Endotoxin

COA for Omnipur Sterile Water

Minimum Essential Medium Eagle (EMEM) with Alpha Modification, powder form

Brand:

Sigma-Aldrich

This is the most enriched variation of the EMEM formulation offered that suitable for cell culuture. It contains all 21 normal amino acids, some at increased concentrations. In addition, it contains 5 additional vitamins. Refer to the table below and choose your preferecd EMEM!

Minimum Essential Medium Eagle (EMEM) with Alpha Modification, powder form Brochure

OEM SeaKem® LE Agarose

Brand:

Lonza

Product Overview
SeaKem® LE Agarose was the first and is still the world’s most trusted agarose for nucleic acid electrophoresis. Ideal for routine analysis of nucleic acids by gel electrophoresis and blotting, each SeaKem® LE Gel sharply resolves DNA and provides consistent resolution from lot-to-lot. This molecular biology grade agarose has no detectable DNase or RNase activity and forms strong gels with low background upon ethidium bromide, SYBR® Green, or GelStarTM Staining. Due to its low EEO, DNA will have a high electrophoretic mobility. SeaKem® LE Agarose is also recommended for protein gel electrophoresis in applications such as Ouchterlony and radial immunodiffusion (RID). Information about bulk and custom packaging is available through Lonza’s Customer Service Team.

Benefits

  • All Lonza Agarose is quality tested to certify performance
  • High gel strength
  • Low EEO
  • Standard melting temperature

Applications

  • Standard DNA/RNA Electrophoreisis

Content & Storage

  • SeaKem® LE Agarose 500 g

1 kb DNA Ladder, NEB

Brand:

New England Biolabs

This 1 kb DNA ladder is available in Quick-Load Purple format – enjoy the convenience of a ready-to-load format.

1 kb DNA ladder from New England Biolabs (NEB) is suitable for use with molecular weight standard for agarose gel electrophoresis. A number of proprietary plasmids are digested to completion with appropriate restriction enzymes to yield 10 bands, ranging from 0.5 to 10 kilobases. NEB set 3.0 kb fragment as a reference band by increasing the intensity of that band.

  • Size range: 500 bp to 10 kb
  • Value pricing
  • Supplied with a free vial of Gel Loading Dye, Purple (6X), no SDS (NEB #B7025).
  • Easy to identify reference bands
  • Small size suitable for 200 gel lanes; large size suitable for 1000 gel lanes

Q5 Hot Start High-Fidelity 2X Master Mix

Brand:

New England Biolabs

Fidelity at its finest

Q5® High-Fidelity DNA Polymerase sets a new standard for both fidelity and robust performance. With the highest fidelity amplification available (~280 times higher than Taq), Q5 DNA Polymerase results in ultra-low error rates. Q5 DNA Polymerase is composed of a novel polymerase that is fused to the processivity-enhancing Sso7d DNA binding domain, improving speed, fidelity and reliability of performance.

  • Highest fidelity amplification (~280X higher than Taq)
  • Ultra-low error rates
  • Superior performance for a broad range of amplicons (from high AT to high GC)
  • Hot start and master mix formats available

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