|Product Code||Description||Product Size||Quantity|
|CM0083B||HOYLE MEDIUM BASE 500g||–||500g|
Hoyle Medium Base 500g
Thermo ScientificTM OxoidTM
- Oxoid Hoyle Medium Base is used for isolation and differentiation of Corynebacterium diphtheriae Types.
- Requires addition of Potassium Tellurite 3.5%, Part No. SR0030J
Hoyle Medium Base, Oxoid Composition
|pH 7.8 ± 0.2 @ 25°C|
Hoyle Medium, Oxoid Preparation:
Suspend 40g in 1 litre of distilled water and bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 55°C and add 50ml of Laked Horse Blood SR0048 and 10ml of 3.5% Potassium Tellurite solution SR0030, mix, and pour plates.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates at 2-8°C.
It should be noted that not all corynebacteria produce the typical colonies described above – so in all cases it is advisable to use Hoyle medium in conjunction with the additional media and tests mentioned above.