Brand:
Thermo ScientificTM OxoidTM
- Oxoid Membrane Clostridium Perfringens Agar Base (m-CP Agar Base) is a chromogenic medium for rapid isolation and presumptive identification of Clostridium perfringens from water samples.
- Also available: Membrane Clostridium Perfringens Selective Supplement, Part No. SR0188E.
Membrane Clostridium Perfringens (M-CP) Agar Base, Oxoid Composition
| Typical Formula * |
gm/litre
|
| Tryptose |
30.0
|
| Yeast extract |
20.0
|
| Sucrose |
5.0
|
| L-cysteine hydrochloride |
1.0
|
| Magnesium sulphate.7H2O |
0.1
|
| Agar |
15.0
|
| Bromocresol purple |
0.04
|
| pH 7.6 ± 0.2 @ 25°C |
|
(M-CP) Agar Base, Oxoid Preparation:
Suspend 35.55g of m-CP Agar Base in 500ml of distilled water Mix well and sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C and aseptically add the contents of one vial of m-CP Selective Supplement reconstituted as directed. Aseptically add the following sterile solutions dissolved in distilled water:
| Component |
Solution Strength |
Volume (ml) |
| Phenolphthalein biphosphate tetrasodium salt |
0.5%
|
10.0
|
| Ferric chloride hexahydrate |
4.5%
|
1.0
|
| Indoxyl b-D-glucoside |
0.75%
|
4.0
|
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Prepared medium may be stored for up to 5 days at 2-8°C in the dark.