| Product Code | Description | Product Size | Quantity | Format |
| CM0992B | (M-CP) Agar Base 500g | – | 500g | Powder |
(M-CP) Agar Base 500g
Brand:
Thermo ScientificTM OxoidTM
- Oxoid Membrane Clostridium Perfringens Agar Base (m-CP Agar Base) is a chromogenic medium for rapid isolation and presumptive identification of Clostridium perfringens from water samples.
- Also available: Membrane Clostridium Perfringens Selective Supplement, Part No. SR0188E.
Membrane Clostridium Perfringens (M-CP) Agar Base, Oxoid Composition
| Typical Formula * | gm/litre |
| Tryptose | 30.0 |
| Yeast extract | 20.0 |
| Sucrose | 5.0 |
| L-cysteine hydrochloride | 1.0 |
| Magnesium sulphate.7H2O | 0.1 |
| Agar | 15.0 |
| Bromocresol purple | 0.04 |
| pH 7.6 ± 0.2 @ 25°C |
(M-CP) Agar Base, Oxoid Preparation:
Suspend 35.55g of m-CP Agar Base in 500ml of distilled water Mix well and sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C and aseptically add the contents of one vial of m-CP Selective Supplement reconstituted as directed. Aseptically add the following sterile solutions dissolved in distilled water:
| Component | Solution Strength | Volume (ml) |
| Phenolphthalein biphosphate tetrasodium salt | 0.5% | 10.0 |
| Ferric chloride hexahydrate | 4.5% | 1.0 |
| Indoxyl b-D-glucoside | 0.75% | 4.0 |
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Prepared medium may be stored for up to 5 days at 2-8°C in the dark.
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