Brand:
Thermo ScientificTM OxoidTM
- Oxoid Schaedler Anaerobe Broth is a thioglycollate-free broth for the general growth of anaerobes.
- For use in blood cultures and antibiotic MIC studies.
Schaedler Anaerobe Broth, Oxoid Composition
| Typical Formula* | gm/litre |
| Tryptone Soya Broth (Oxoid CM129) | 10.0 |
| Special peptone | 5.0 |
| Yeast extract | 5.0 |
| Glucose | 5.0 |
| Cysteine HCl | 0.4 |
| Haemin | 0.01 |
| Tris Buffer | 0.75 |
| pH 7.6 ± 0.2 @ 25°C | |
Schaedler Anaerobe Broth, Oxoid Preparation:
Add 26.5g to 1 litre of distilled water and mix to dissolve completely. Dispense into final containers and sterilise by autoclaving at 121°C for 15 minutes.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store prepared broth in the dark at <15°C.
Precautions
As with all anaerobic broth media, it is important to avoid chemo-oxidation (overheating) and photo-oxidation (storage in the light) because such oxidative effects cause the inhibition of growth.