Product Code | Description | Quantity |
CM0671B | Tetrathionate Broth (USA) 500g | 500g |
Tetrathionate Broth (USA) 500g
Brand:
Thermo ScientificTM OxoidTM
Oxoid Tetrathionate Broth is used for the selective enrichment of Salmonella spp.
Tetrathionate Broth, Oxoid Composition
Typical Formula* | gm/litre |
Casein peptone | 2.5 |
Meat peptone | 2.5 |
Bile salts | 1.0 |
Calcium carbonate | 10.0 |
Sodium thiosulphate | 30.0 |
Tetrathionate Broth, Oxoid Preparation:
Suspend 46g in 1 litre of distilled water and bring to the boil. Cool to below 45°C and add 20ml of iodine-iodide solution immediately before use. Mix continuously whilst dispensing 10ml volumes into sterile tubes. Use the complete medium (with added iodine) on the day of preparation.
Iodine-Iodide Solution | |
Iodine | 6g |
Potassium iodide | 5g |
Distilled water | 20ml |
The base may be prepared beforehand and kept for several weeks at 4°C. Iodine-iodide solution can then be added at the time of use to the quantity of medium needed.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the base broth at 2-8°C. Use the medium immediately after adding the iodine solution.
Dimensions | 10 cm |
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Brand | Oxoid |
Estimated Delivery Time | 1-2 weeks |