Tinsdale Medium 500g

Oxoid Tinsdale Agar Base is used for the isolation and identification of Corynebacterium diphtheriae.

Tinsdale Agar Base, Oxoid Composition

Tinsdale Agar, Oxoid Preparation:
Suspend 9g of agar base in 200ml of distilled water. Bring to the boil and dissolve completely. DO NOT AUTOCLAVE. Allow to cool to approximately 50°C and add the contents of one vial of Oxoid Tinsdale Supplement SR0065 reconstiuted as directed. Mix throughly and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C for not more than 4 days.

Precautions
Further tests must be carried out on colonies suspected as Corynebacterium diphtheriae, including cell morphology after sub-culture to Loeffler’s Medium and examination for toxin production.
Do not incubate Tinsdale’s Agar plates in enhanced CO2 atmosphere (5-10% v/v).