4-Nitrophenyl butyrate, ≥98% (Sigma-Aldrich)

Description

CAS number: 2635-84-9
Chemical Formula: C10H11NO4
Molar Mass: 209.20 g/mol
Synonyms: p-Nitrophenyl butyrate, Butyric acid 4-nitrophenyl ester

General description

4-Nitrophenyl butyrate is a substrate for esterase, and lipase enzyme. Hydrolysis of 4-nitrophenyl butyrate by these enzymes releases the chromophore, 4-nitrophenolate which is spectrophotometrically analysed at 415 nm.

Application

4-nitrophenyl butyrate has been used:
• as a substrate for esterase activity in monocyte-derived macrophage cell line in response to polyethylene
• as a substrate for mycobacterial phospholipase A and mycobacterial cutinase activity
• as a substrate to determine the effect of buffers and solvents on human carboxylesterase