Baird Parker Agar Base 500g, Oxoid

Baird Parker Agar Base, Oxoid is suitable for the isolation and enumeration of Staphylococcus aureus, with high selectivity and sensitivity. It contains carefully balanced selective inhibitory agents, glycine, lithium, and tellurite, to suppress the growth of most of the bacteria present in foods without inhibiting coagulase-positive staphylococci. The presence of egg yolk emulsion and sodium pyruvate aid the recovery of damaged cells for presumptive identification of Staphylococcus aureus in food specimens.

Baird Parker Agar Base, Oxoid Preparation:

Suspend 6.3g of Baird-Parker Agar Base (RPF) in 90ml of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 121ºC for 15 minutes. Cool to 48ºC and add 1 vial of RPF Supplement (SR0122A), reconstituted as directed. Mix well and pour plates.

Storage Conditions and Shelf Life
Store the dehydrated medium at 10-30°C and RPF Supplement below 0°C. Use before the expiry date on the label.
Prepared plates of the medium are best used freshly prepared.

Precautions
Regard all suspicious colonies as Staphylococcus aureus regardless of negative reactions in the medium and carry out further tests.
Colonies of some contaminating organisms growing in close proximity to the coagulase positive colonies may partially digest the coagulase halo reaction.