| Product Code | Description | Product Size | Quantity |
| CM0983B | Bolton Broth 500g | – | 500g |
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Bolton Broth 500g
RM517.00
Brand:
Thermo ScientificTM OxoidTM
Selectively pre-enrich Campylobacter in food samples with Thermo Scientific™ Oxoid™ Bolton Broth Base (Dehydrated). Avoid false-negative results due to sub-lethally injured cells with Bolton Broth Base as it increases the number of cells available for culture, first by resuscitating organisms and then by encouraging them to multiply.
Bolton Broth Base, Oxoid Composition
| Typical Formula* |
gm/litre |
| Meat peptone |
10.0 |
| Lactalbumin hydrolysate |
5.0 |
| Yeast Extract |
5.0 |
| Sodium chloride |
5.0 |
| Alpha-ketoglutaric acid |
1.0 |
| Sodium pyruvate |
0.5 |
| Sodium metabisulphite |
0.5 |
| Sodium carbonate |
0.6 |
| Haemin |
0.01 |
| pH 7.4 ± 0.2 @ 25°C |
Bolton Broth, Oxoid Preparation:
Add 13.8g of Bolton Broth to 500ml of distilled water. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C. Aseptically add 25ml Laked Horse Blood SR0048 and 1 vial of Bolton Broth Selective Supplement SR0183, reconstituted as directed. Mix well and distribute into sterile screw top containers.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C for up to 2 weeks.
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Description
Additional information
| Dimensions | 10 cm |
|---|---|
| Brand | Oxoid |
| Estimated Delivery Time | 1-2 weeks |
