Product Code | Description | Product Size | Packaging |
CM1212B | BrillianceTM Listeria Agar (ISO) Base | – | 500g |
Thermo ScientificTM OxoidTM BrillianceTM Listeria Agar (ISO) Base
Brand:
Thermo ScientificTM OxoidTM
Isolate, enumerate and presumptively identify Listeria species and L. monocytogenes from food and environmental samples with the Thermo Scientific™ Oxoid™ Brilliance™ Listeria Agar (ISO) (Dehydrated culture medium and supplements) following broth enrichment. The medium identifies Listeria species based on their utilization of X-glucoside giving rise to blue-green colonies. Listeria monocytogenes are further differentiated by their ability to highly produce lecithinase, producing an opaque, white halo around the colony.
Brilliance Listeria Agar (ISO), Oxoid Composition
Typical Formula * | gm/litre |
Enzymatic digest of animal tissues | 18.0 |
Enzymatic digest of casein | 6.0 |
Yeast extract | 10.0 |
Sodium pyruvate | 2.0 |
Glucose | 2.0 |
Magnesium glycerophosphate | 1.0 |
Magnesium sulphate (anhydrous) | 0.5 |
Sodium chloride | 5.0 |
Lithium chloride | 10.0 |
Di-sodium hydrogen phosphate (anhydrous) | 2.5 |
5-Bromo-4-chloro-3-indolyl-β-D-glucopyranoside | 0.05 |
Agar | 12.0 |
pH 7.4 ± 0.2 @ 25°C |
Brilliance Listeria Agar (ISO), Oxoid Preparation:
Suspend 34.5g in 480ml of distilled water. Mix well and sterilize by autoclaving at 121°C for 15 minutes. Cool to 48°C. Aseptically add the contents of 1 vial of Brilliance™ Listeria Selective Supplement (ISO) (SR0257E) reconstituted as directed, and 1 vial of Brilliance™ Listeria Differential Supplement (ISO) (SR0258E) warmed to 48°C. Mix well and pour into sterile Petri dishes.
Storage conditions and Shelf life
Store the dehydrated medium (CM1212B) at 10-30°C away from light and ensure bottles are correctly sealed after use.
Store prepared medium for up to 2 weeks at 2-8°C, away from light. Ensure all media is used before the expiry date on the label
Dimensions | 10 cm |
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Brand | Oxoid |
Estimated Delivery Time | 1-2 weeks |