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HomeMicrobiologyCulture Media Products Thermo ScientificTM OxoidTM BrillianceTM Listeria Agar (ISO) Base
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Thermo ScientificTM OxoidTM Brilliance Listeria Agar (ISO) Selective Supplement
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oxoid

Thermo ScientificTM OxoidTM BrillianceTM Listeria Agar (ISO) Base

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Thermo ScientificTM OxoidTM

Isolate, enumerate and presumptively identify Listeria species and L. monocytogenes from food and environmental samples with the Thermo Scientific™ Oxoid™ Brilliance™ Listeria Agar (ISO) (Dehydrated culture medium and supplements) following broth enrichment. The medium identifies Listeria species based on their utilization of X-glucoside giving rise to blue-green colonies. Listeria monocytogenes are further differentiated by their ability to highly produce lecithinase, producing an opaque, white halo around the colony.

Brilliance Listeria Agar (ISO), Oxoid Composition

Typical Formula *
gm/litre
Enzymatic digest of animal tissues

18.0

Enzymatic digest of casein

6.0

Yeast extract

10.0

Sodium pyruvate

2.0

Glucose

2.0

Magnesium glycerophosphate

1.0

Magnesium sulphate (anhydrous)

0.5

Sodium chloride

5.0

Lithium chloride

10.0

Di-sodium hydrogen phosphate (anhydrous)

2.5

5-Bromo-4-chloro-3-indolyl-β-D-glucopyranoside

0.05

Agar

12.0

pH 7.4 ± 0.2 @ 25°C

Brilliance Listeria Agar (ISO), Oxoid Preparation:

Suspend 34.5g in 480ml of distilled water. Mix well and sterilize by autoclaving at 121°C for 15 minutes. Cool to 48°C. Aseptically add the contents of 1 vial of Brilliance™ Listeria Selective Supplement (ISO) (SR0257E) reconstituted as directed, and 1 vial of Brilliance™ Listeria Differential Supplement (ISO) (SR0258E) warmed to 48°C. Mix well and pour into sterile Petri dishes.

Storage conditions and Shelf life
Store the dehydrated medium (CM1212B) at 10-30°C away from light and ensure bottles are correctly sealed after use.

Store prepared medium for up to 2 weeks at 2-8°C, away from light. Ensure all media is used before the expiry date on the label

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SKU: CM1212B Categories: Culture Media Products, Microbiology
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  • Description
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Description
Product CodeDescriptionProduct SizePackaging
CM1212BBrillianceTM Listeria Agar (ISO) Base–500g
Additional information
Dimensions10 cm
Brand

Oxoid

Estimated Delivery Time

1-2 weeks

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Thermo ScientificTM OxoidTM BrillianceTM Listeria Agar (ISO) Base

Thermo ScientificTM OxoidTM BrillianceTM Listeria Agar (ISO) Base

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