Brand:
Thermo ScientificTM OxoidTM
Isolate, enumerate and presumptively identify Listeria species and L. monocytogenes from food and environmental samples with the Thermo Scientific⢠Oxoidâ¢Â Brillianceâ¢Â Listeria Agar (ISO) (Dehydrated culture medium and supplements) following broth enrichment. The medium identifies Listeria species based on their utilization of X-glucoside giving rise to blue-green colonies. Listeria monocytogenes are further differentiated by their ability to highly produce lecithinase, producing an opaque, white halo around the colony.
Brilliance Listeria Agar (ISO), Oxoid Composition
| Typical Formula * |
gm/litre
|
| Enzymatic digest of animal tissues |
18.0
|
| Enzymatic digest of casein |
6.0
|
| Yeast extract |
10.0
|
| Sodium pyruvate |
2.0
|
| Glucose |
2.0
|
| Magnesium glycerophosphate |
1.0
|
| Magnesium sulphate (anhydrous) |
0.5
|
| Sodium chloride |
5.0
|
| Lithium chloride |
10.0
|
| Di-sodium hydrogen phosphate (anhydrous) |
2.5
|
| 5-Bromo-4-chloro-3-indolyl-β-D-glucopyranoside |
0.05
|
| Agar |
12.0
|
| pH 7.4 ± 0.2 @ 25°C |
|
Brilliance Listeria Agar (ISO), Oxoid Preparation:
Suspend 34.5g in 480ml of distilled water. Mix well and sterilize by autoclaving at 121°C for 15 minutes. Cool to 48°C. Aseptically add the contents of 1 vial of Brilliance⢠Listeria Selective Supplement (ISO) (SR0257E) reconstituted as directed, and 1 vial of Brilliance⢠Listeria Differential Supplement (ISO) (SR0258E) warmed to 48°C. Mix well and pour into sterile Petri dishes.
Storage conditions and Shelf life
Store the dehydrated medium (CM1212B) at 10-30°C away from light and ensure bottles are correctly sealed after use.
Store prepared medium for up to 2 weeks at 2-8°C, away from light. Ensure all media is used before the expiry date on the label