|DEHYDRATED ROGOSA AGAR 500g
Dehydrated Rogosa Agar 500g
Thermo ScientificTM OxoidTM
Oxoid Rogosa Agar is used for the selective isolation and enumeration of lactobacilli.
Rogosa Agar, Oxoid Composition
|Sorbitan mono-oleate `Tween 80’
|Potassium dihydrogen phosphate
|Sodium acetate, anhydrous
|pH 5.4 ± 0.2 @ 25°C
Rogosa Agar, Oxoid Preparation:
Suspend 82g in 1 litre of distilled water and bring to the boil to dissolve completely. Add 1.32ml glacial acetic acid and mix thoroughly. Heat to 90-100°C for 2-3 minutes with frequent agitation. Distribute into sterile tubes, Petri dishes or bottles.
DO NOT AUTOCLAVE.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.
Lactobacillus carnis does not grow on this medium.
All colonies must be tested by Gram stain and catalase test before carrying out further identification tests.