|Fraser Broth 500g
Fraser Broth 500g
Thermo ScientificTM OxoidTM
Isolate Listeria spp. from food and environmental specimens using Thermo Scientific™ Oxoid™ Fraser Broth Base (Dehydrated). The medium is intended for the isolation of Listeria spp. when used as the secondary enrichment medium in the USDA-FSIS methodology for Listeria isolation. Blackening of the medium is presumptive evidence of the presence of Listeria spp. All Fraser Broth enrichment cultures should be sub-cultured to plating medium.
Fraser Broth Base, Oxoid Composition
|Di-sodium hydrogen phosphate
|Potassium dihydrogen phosphate
|pH 7.2 ± 0.2 @ 25°C
Fraser Broth, Oxoid Preparation:
With Fraser Supplement:
Suspend 28.7g in 500ml of distilled water and mix well to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Cool to below 50°C, and aseptically add the contents of one vial of Fraser Selective Supplement (SR0156) reconstituted as directed in the product insert. Mix well and distribute into sterile containers.
With Half Fraser Supplement:
Suspend 12.9g of Fraser Broth in 225ml of distilled water., and mix well to dissolve completely Sterilise by autoclaving at 121°C for 15 minutes. Cool to below 50°C, and aseptically add the contents of one vial of Half Fraser Selective Supplement SR0166E reconstituted as directed in the product insert. Mix well and distribute into sterile containers. Alternatively, to make 2.25 litres, use 129.0g of CM0895 and 1 vial of SR0166G.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
The prepared medium may be stored for up to 2 weeks at 2-8°C.