Brand:
Thermo ScientificTM OxoidTM
Perform effortless testing using Thermo Scientific⢠Oxoid⢠Iso-Sensitest Broth (Dehydrated), a semi-defined medium specifically designed for antimicrobial susceptibility testing (AST). Iso-Sensitest Broth is carefully constructed to give reproducible results. It allows the growth of the majority of microorganisms without the need of further supplementation.
Iso-Sensitest Broth, Oxoid Composition
| Typical Formula* |
gm/litre
|
| Hydrolysed casein |
11.0
|
| Peptones |
3.0
|
| Glucose |
2.0
|
| Sodium chloride |
3.0
|
| Soluble starch |
1.0
|
| Disodium hydrogen phosphate |
2.0
|
| Sodium acetate |
1.0
|
| Magnesium glycerophosphate |
0.2
|
| Calcium gluconate |
0.1
|
| Cobaltous sulphate |
0.001
|
| Cupric sulphate |
0.001
|
| Zinc sulphate |
0.001
|
| Ferrous sulphate |
0.001
|
| Manganous chloride |
0.002
|
| Menadione |
0.001
|
| Cyanocobalamin |
0.001
|
| L-Cysteine hydrochloride |
0.02
|
| L-Tryptophan |
0.02
|
| Pyridoxine |
0.003
|
| Pantothenate |
0.003
|
| Nicotinamide |
0.003
|
| Biotin |
0.0003
|
| Thiamine |
0.00004
|
| Adenine |
0.01
|
| Guanine |
0.01
|
| Xanthine |
0.01
|
| Uracil |
0.01
|
| pH 7.4 ± 0.2 @ 25°C |
|
Iso-Sensitest Broth, Oxoid Preparation:
Add 23.4g to 1 litre of distilled water. Mix well and heat to dissolve. Distribute into tubes or bottles. Sterilise by autoclaving at 121°C for 15 minutes.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.