Brand:
Thermo ScientificTM OxoidTM
Isolate and enumerate lactic acid bacteria from food with Thermo Scientific⢠Oxoid⢠MRS Broth (DeMan, Rogosa and Sharpe) (Dehydrated). This non-selective medium is designed to support profuse growth of lactic acid bacteria.
MRS Broth (DeMan, Rogosa and Sharpe), Oxoid Composition
| Typical Formula* |
gm/litre
|
| Peptone |
10.0
|
| `Lab-Lemcoâ powder |
8.0
|
| Yeast extract |
4.0
|
| Glucose |
20.0
|
| Sorbitan mono-oleate |
1 ml
|
| Dipotassium hydrogen phosphate |
2.0
|
| Sodium acetate 3H2O |
5.0
|
| Triammonium citrate |
2.0
|
| Magnesium sulphate 7H2O |
0.2
|
| Manganese sulphate 4H2O |
0.05
|
| pH 6.2 ± 0.2 @ 25°C |
|
MRS Broth (DeMan, Rogosa and Sharpe), Oxoid Preparation:
Add 52g to 1 litre of distilled water at approximately 60°C. Mix until completely dissolved. Dispense into final containers and sterilise by autoclaving at 121°C for 15 minutes.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.