Brand:
Thermo ScientificTM OxoidTM
Presumptively detect, isolate and enumerate pathogenic staphylococci in milk, food, fish and other specimens with Thermo Scientific⢠Oxoid⢠Mannitol Salt Agar (Dehydrated). Due to high concentrations of salt in the medium, most unwanted bacteria are inhibited with the exception of some halophilic marine organisms. Presumptive coagulase-positive staphylococci produce colonies surrounded by bright yellow zones while non-pathogenic staphylococci produce colonies with reddish purple zones.
Mannitol Salt Agar, Oxoid Composition
| Typical Formula* | gm/litre |
| `Lab-Lemcoâ powder | 1.0 |
| Peptone | 10.0 |
| Mannitol | 10.0 |
| Sodium chloride | 75.0 |
| Phenol red | 0.025 |
| Agar | 15.0 |
| pH 7.5 ± 0.2 @ 25°C | |
Mannitol Salt Agar, Oxoid Preparation:
Suspend 111g in 1 litre of distilled water and bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates at 2-8°C.