Q5U® Hot Start High-Fidelity DNA Polymerase, NEB

Q5U^® Hot Start High-Fidelity DNA Polymerase is a modified version of Q5^® High-Fidelity DNA Polymerase, a novel thermostable DNA polymerase that possesses 3′ to 5′ exonuclease activity, and is fused to a processivity-enhancing Sso7d domain. Q5U^® contains a mutation in the uracil-binding pocket that enables the ability to read and amplify templates containing uracil and inosine bases.

• Achieve superior amplification of bisulfite-converted, deaminated, or damaged DNA (e.g., FFPE)
• Prevent carryover contamination when combined with dUTP and Uracil DNA Glycosylase (UDG) treatment
• Generate higher assembly efficiency and improve accuracy in USER® cloning
• Enable room temperature setup with aptamer-based hot start formulation
• Utilize optimized protocols for recommended applications