|SLANETZ AND BARTLEY MEDIUM 500g
Slanetz And Bartley Medium 500g
Thermo ScientificTM OxoidTM
Detect and enumerate enterococci from water and food samples with Thermo Scientific™ Slanetz and Bartley Medium (Dehydrated). Slanetz and Bartley originally devised this medium to detect and enumerate enterococci by the technique of membrane filtration, but it has also proved useful as a direct plating medium. The medium is very selective for enterococci and, when it is incubated at elevated temperatures (44-45°C), all red or maroon colonies may be accepted as presumptive enterococci.
Slanetz and Bartley Medium (Dehydrated), Oxoid Composition
|Di-potassium hydrogen phosphate
|pH 7.2 ± 0.2 @ 25°C
Slanetz and Bartley Medium Preparation:
Suspend 42g in 1 litre of distilled water and bring to the boil to dissolve the agar completely.
EXCESSIVE HEATING MUST BE AVOIDED. Dispense into Petri dishes and allow to solidify. It should not be remelted. The medium may be used with membrane filters or by spreading dilutions of the sample over the surface of the agar with a glass rod.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C away from light.
Count all red, maroon or pink colonies as presumptive enterococci. Not all species reduce TTC therefore pale colonies should not be ignored.
Although incubation at 35°C yields a higher count, it allows the growth of organisms which do not conform to the definition of enterococci. Incubation at 44-45°C has a selective effect and produces fewer false-positives. However, the preliminary incubation at 35°C encourages the recovery of stressed organisms.
Although the selective properties of this medium are very good it is advisable to regard the colony count as a presumptive or unconfirmed count. Further identification may be required depending on the scope of the examination.