Product Code | Description | Product Size | Quantity |
CM1134B | E.Sak Isolation Agar Base 500g | – | 500g |
E.Sak Isolation Agar Base 500g
Brand:
Thermo ScientificTM OxoidTM
Oxoid Cronobacter Sakazakii Isolation Agar (ISO) is a chromogenic medium for enrichment and isolation of Cronobacter spp. from milk and milk products in compliance with ISO/TS 22964: 2006.
E.Sak Isolation Agar Base, Oxoid Composition
Typical Formula* | gm/litre |
Pancreatic digest of casein† | 7.0 |
Yeast extract | 3.0 |
Sodium chloride | 5.0 |
Sodium deoxycholate†† | 0.6 |
5-Bromo-4-chloro-3-indolyl α-D-glucopyranoside | 0.15 |
Crystal violet | 0.002 |
Agar | 12.0 |
pH 7.0 ± 0.2 @ 25°C |
E.Sak Isolation Agar, Oxoid Preparation:
Suspend 27.75g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilize by autoclaving at 121°C for 15 minutes. Cool to approximately 50°C. Mix well and pour into sterile Petri dishes.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
The prepared medium is best used freshly prepared, but may be suitable for longer storage following in-house validation.
Dimensions | 10 cm |
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Brand | Oxoid |
Estimated Delivery Time | 1-2 weeks |