|Brilliance Cronobacter Sakazakii Agar (DFI) 500g
Brilliance Cronobacter Sakazakii Agar (DFI) 500g
Thermo ScientificTM OxoidTM
Differentiate and enumerate Cronobacter sakazakii from infant formula and other food samples with Thermo Scientific™ Brilliance™ Cronobacter Sakazakii Agar (Dehydrated). A chromogenic substrate present in the medium detects a-glucosidase reaction in Cronobacter sakazakii. The enzyme a-glucosidase, present in Cronobacter sakazakii, hydrolyses the substrate producing blue-green colonies on this pale yellow medium.
Brilliance Cronobacter Sakazakii Agar, Oxoid Composition
|Typical Formula *
|Ferric ammonium citrate
|Final pH 7.3 ± 0.2 @ 25°C
Brilliance Cronobacter Sakazakii Agar, Oxoid Preparation:
Suspend 43.1g of Brilliance Cronobacter sakazakii Agar in 1 litre of distilled water. Mix well and bring to the boil to dissolve completely. Sterilize by autoclaving at 121°C for 15 minutes. Cool the medium to 50°C. Mix well and pour into sterile Petri dishes.
Storage conditions and Shelf life
The dehydrated medium should be stored at 10-30°C and used before the expiry date on the label.
Prepared medium may be stored for up to 2 weeks at 2-8°C.