|BIGGY AGAR 500g
Biggy Agar 500g
Thermo ScientificTM OxoidTM
Oxoid BiGGY Agar (Nickerson Medium) for the isolation and presumptive identification of Candida spp.
BiGGY Agar, Oxoid Composition
|Bismuth ammonium citrate
|pH 6.8 ± 0.2 @ 25°C
BiGGY Agar, Oxoid Preparation:
Suspend 42g in 1 litre of distilled water and bring gently to the boil to dissolve the agar. Allow to cool to 50-55°C. Mix gently to disperse the flocculant precipitate and pour into sterile Petri dishes.
DO NOT AUTOCLAVE THE MEDIUM.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Medium should be freshly prepared just prior to use.
Carry out further tests to confirm identity of isolated yeasts.
Do not use slants of medium because the reactions are unsatisfactory.
The flocculent precipitate present in the molten medium must be evenly suspended whilst dispensing the agar.