Brand:
Thermo ScientificTM OxoidTM
Isolate, enumerate and differentiate urinary pathogens with Thermo Scientific⢠Oxoid⢠C.L.E.D. Medium (Dehydrated). Cystine-Lactose-Electrolyte-Deficient (CLED) Medium which provides good colonial differentiation, clear diagnostic characteristics and prevents swarming of Proteus spp. due to the electrolyte deficiency in the formulation.
C.L.E.D. Medium, Oxoid Composition
| Typical Formula* | gm/litre |
| Peptone | 4.0 |
| `Lab-Lemcoâ powder | 3.0 |
| Tryptone | 4.0 |
| Lactose | 10.0 |
| L-cystine | 0.128 |
| Bromothymol blue | 0.02 |
| Agar | 15.0 |
| pH 7.3 ± 0.2 @ 25°C | |
C.L.E.D. Medium, Oxoid Preparation:
Suspend 36.2g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Mix well before pouring.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°Cand use before the expiry date on the label.
Store the prepared medium at 2-8°C.