D.C.L.S. Agar 500g

DCLS Agar is a modified form of Desoxycholate Citrate Agar which includes sucrose in its formulation. The addition of this fermentable carbohydrate increases the usefulness of the medium because non-pathogenic sucrose-fermenting organisms may be recognised by their red colonies, e.g. some Proteus, Enterobacter and Klebsiella species.

DCLS Agar, Oxoid Composition

DCLS Agar, Oxoid Preparation:
Suspend 50g in 1 litre of distilled water. Bring to the boil to dissolve the medium. Cool to 50°C and pour into sterile Petri dishes.
DO NOT AUTOCLAVE.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared agar plates at 2-8°C.

Precautions
Boil the medium for the minimal period of time to get the agar into solution. Overheating reduces the agar gel strength and increases the degree of inhibition. It is therefore important not to hold the molten medium at 50°C for more than the short time required to distribute it into dishes.