|Product Code||Description||Product Size||Quantity|
|CM0343B||MULLER KAUFFMANN TETRATH BROTH 500g||–||500g|
Muller Kauffmann Tetrathionate Broth 500g
Thermo ScientificTM OxoidTM
Oxoid Muller-Kauffmann Tetrathionate Broth Base is selective enrichment medium for isolation of Salmonella spp. and suppression of Proteus species.
Muller-Kauffmann Tetrathionate Broth Base, Oxoid Composition
|pH 8.0 ± 0.2 @ 25°C|
Muller-Kauffmann Tetrathionate Broth, Oxoid Preparation:
Suspend 82g in 1 litre of distilled water and bring to the boil. Cool below 45°C and add, just prior to use, 19ml of iodine solution and 9.5ml of a 0.1% brilliant green solution. Mix well and fill out into sterile tubes or flasks.
|Distilled water to|
Dissolve the potassium iodide in approximately 5ml of distilled water, add the iodide and gently warm the solution to completely dissolve it. Make up the volume to 100ml with distilled water.
|Brilliant Green Solution|
|Brilliant Green (BDH or Chroma)|
Add the brilliant green to the distilled water and shake to dissolve the dye. Heat the solution to 100°C for 30 minutes and shake from time to time whilst cooling, to ensure that the dye has completely dissolved. Store in a brown glass bottle or away from light.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.
Do not autoclave the base broth.
Add the iodine solution and brilliant green just prior to use.
The medium is not suitable for the growth of Salmonella typhi, Salmonella sendai, Salmonella pullorum and Salmonella gallinarum.