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HomeMicrobiologyCulture Media Products Muller Kauffmann Tetrathionate Broth 500g
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Muller Kauffmann Tetrathionate Broth 500g

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Brand:

Thermo ScientificTM OxoidTM

Oxoid Muller-Kauffmann Tetrathionate Broth Base is selective enrichment medium for isolation of Salmonella spp. and suppression of Proteus species.

Muller-Kauffmann Tetrathionate Broth Base, Oxoid Composition

Typical Formula*

gm/litre

Tryptone

7.0

Soya peptone

2.3

Sodium chloride

2.3

Calcium carbonate

25.0

Sodium thiosulphate

40.7

Ox bile
4.75
pH 8.0 ± 0.2 @ 25°C

 

Muller-Kauffmann Tetrathionate Broth, Oxoid Preparation:
Suspend 82g in 1 litre of distilled water and bring to the boil. Cool below 45°C and add, just prior to use, 19ml of iodine solution and 9.5ml of a 0.1% brilliant green solution. Mix well and fill out into sterile tubes or flasks.

Iodine Solution
Iodine

20g

Potassium iodide

25g

Distilled water to

100ml

Dissolve the potassium iodide in approximately 5ml of distilled water, add the iodide and gently warm the solution to completely dissolve it. Make up the volume to 100ml with distilled water.

Brilliant Green Solution
Brilliant Green (BDH or Chroma)

0.1g

Distilled water

100ml

Add the brilliant green to the distilled water and shake to dissolve the dye. Heat the solution to 100°C for 30 minutes and shake from time to time whilst cooling, to ensure that the dye has completely dissolved. Store in a brown glass bottle or away from light.

Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.

Precautions
Do not autoclave the base broth.
Add the iodine solution and brilliant green just prior to use.
The medium is not suitable for the growth of Salmonella typhi, Salmonella sendai, Salmonella pullorum and Salmonella gallinarum.

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SKU: CM0343B Categories: Culture Media Products, Microbiology
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Description
Product CodeDescriptionProduct SizeQuantity
CM0343BMULLER KAUFFMANN TETRATH BROTH 500g–500g
Additional information
Dimensions10 cm
Brand

Oxoid

Estimated Delivery Time

1-2 weeks

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Muller Kauffmann Tetrathionate Broth 500g

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